Metabolic effects of azoxystrobin and kresoxim-methyl against Fusarium kyushuense examined using the Biolog FF MicroPlate

Wang, Han-Cheng; Jin, Wang; Chen, Qingyuan; Wang, Maosheng; Hsiang, Tom; Shang, Shenghua; Yu, Zhihe

doi:10.1016/j.pestbp.2015.11.013

Cited by 24 publications

(16 citation statements)

References 23 publications

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“…The upregulation proteins in zoospores responding to common bean seed exudates participate in carbon metabolism, sulphur metabolism, amino acid synthesis and glycine-serine-threonine-methionine-cysteine metabolism. Many fungicides affect the carbon metabolism process of pathogens through the above processes (Wang et al 2016). During the response of zoospores to nonhost seed exudates, glutathione-Px and glutathione-S-transferase are downregulated, resulting in the inhibition of GSH oxidation process, the enhancement of glutathione reduction process and the increasing glutathione to mediate nonhost resistance.…”

Section: Discussionmentioning

confidence: 99%

Maize and Common Bean Seed Exudates Mediate Part of Nonhost Resistance to Phytophthora Sojae before Infection

Zhang

Zhao

et al. 2021

Preprint

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Purpose: Phytophthora sojae does not infect the nonhost maize (Zea mays L.) but could infect the nonhost common bean (Phaseolus vulgaris L.). Soybean seed exudates participate in mediating host resistance to P. sojae before infection. This study aimed to elucidate the role of nonhost seed exudates in mediating nonhost resistance to P. sojae before infection. Methods: The response behavior of P. sojae zoospores to the seed exudates was determined using an assay chamber and a concave slide, and the proteomes of P. sojae zoospores treated with the seed exudates were analysed with the tandem mass tag (TMT) method. The key proteins were quantified by parallel reaction monitoring (PRM).Results: Maize seed exudates exerted a repellent effect on zoospores, whereas common bean seed exudates did not exhibit any attraction to zoospores but could dissolve the cysts. The key proteins related to zoospores chemotaxis showed no significant changes in response to maize seed exudates, but the key proteins in arachidonic acid pathway were downregulated and controlled the repellent behavior of zoospores. Proteins protecting the cell membrane structure were significantly downregulated in zoospores responding to common bean seed exudates, which confirmed the bacteriolytic effect of common bean seed exudates on cysts. Conclusion: Maize and common bean seed exudates mediate part of the nonhost resistance via different mechanisms prior to P. sojae infection. The immune of maize to P. sojae is due to the repellent effect of maize seed exudates on zoospores. Common bean seed exudates participate in mediating nonhost resistance by dissolving cysts.

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Section: Discussionmentioning

confidence: 99%

Maize and Common Bean Seed Exudates Mediate Part of Nonhost Resistance to Phytophthora Sojae before Infection

Zhang

Zhao

et al. 2021

Preprint

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“…For the biological identification of suspect isolates, filamentous fungi inoculating fluid (containing 2.5 g L -1 Phytagel and 0.3 g L -1 Tween 40) (FF-IF, catalogue # 72106) and FF MicroPlate test panels (catalogue # 1006) were used ( Wang et al, 2016b ). All of these materials were all purchased from Biolog, Inc. (Hayward, CA, United States).…”

Section: Methodsmentioning

confidence: 99%

Metabolic Phenotype Characterization of Botrytis cinerea, the Causal Agent of Gray Mold

Wang

Cai

et al. 2018

Front. Microbiol.

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Botrytis cinerea, which causes gray mold, is an important pathogen in four important economic crops, tomato, tobacco, cucumber and strawberry, in China and worldwide. Metabolic phenomics data on B. cinerea isolates from these four crops were characterized and compared for 950 phenotypes with a BIOLOG Phenotype MicroArray (PM). The results showed that the metabolic fingerprints of the four B. cinerea isolates were similar to each other with minimal differences. B. cinerea isolates all metabolized more than 17% of the tested carbon sources, 63% of the amino acid nitrogen substrates, 80% of the peptide nitrogen substrates, 93% of the phosphorus substrates, and 97% of the sulfur substrates. Carbon substrates of organic acids and carbohydrates, and nitrogen substrates of amino acids and peptides were the significant utilization patterns for B. cinerea. Each B. cinerea isolate contained 94 biosynthetic pathways. These isolates showed a large range of adaptabilities and were still able to metabolize substrates in the presence of the osmolytes, including up to 6% potassium chloride, 10% sodium chloride, 5% sodium sulfate, 6% sodium formate, 20% ethylene glycol, and 3% urea. These isolates all showed active metabolism in environments with pH values from 3.5 to 8.5 and exhibited decarboxylase activities. These characterizations provide a theoretical basis for the study of B. cinerea in biochemistry and metabolic phenomics and provide valuable clues to finding potential new ways to manage gray mold.

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“…Many carbon compounds could be metabolized. The number of carbon substrates metabolized by B. cinerea is higher than that of other pathogens, including Fusarium kyushuense 21 . The most informative utilization patterns for carbon sources were saccharides.…”

Section: Discussionmentioning

confidence: 86%

“…The carbon substrate utilization help to elucidate the mode of action of each test chemical. There have been a few previous reports of its use for investigating mode of action of fungicides 20 21 , and none for these botryticides.…”

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confidence: 99%

Metabolic activities of five botryticides against Botrytis cinerea examined using the Biolog FF MicroPlate

Wang¹,

Jin

et al. 2016

Sci Rep

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Tobacco grey mold caused by Botrytis cinerea is an important fungal disease worldwide. Boscalid, carbendazim, iprodione, pyrimethanil and propiconazole are representative botryticides for grey mold management. This research investigated the sensitivities of B. cinerea from tobacco to these chemicals using the Biolog FF Microplate. All five chemicals showed inhibitory activity, with average EC50 values of 0.94, 0.05, 0.50, 0.61 and 0.31 μg ml−1, respectively. B. cinerea metabolized 96.8% of tested carbon sources, including 29 effectively and 33 moderately, but the metabolic fingerprints differed under pressures imposed by these botryticides. For boscalid, B. cinerea was unable to metabolize many substrates related to tricarboxylic acid cycle. For carbendazim, carbon sources related to glycolysis were not metabolized. For iprodione, use of most carbon substrates was weakly inhibited, and the metabolic profile was similar to that of the control. For propiconazole, no carbon substrates were metabolized and the physiological and biochemical functions of the pathogen were totally inhibited. These findings provide useful information on metabolic activities of these botryticides, and may lead to future applications of the Biolog FF Microplate for examining metabolic effects of other fungicides on other fungi, as well as providing a metabolic fingerprint of B. cinerea that could be useful for identification.

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Metabolic effects of azoxystrobin and kresoxim-methyl against Fusarium kyushuense examined using the Biolog FF MicroPlate

Cited by 24 publications

References 23 publications

Maize and Common Bean Seed Exudates Mediate Part of Nonhost Resistance to Phytophthora Sojae before Infection

Maize and Common Bean Seed Exudates Mediate Part of Nonhost Resistance to Phytophthora Sojae before Infection

Metabolic Phenotype Characterization of Botrytis cinerea, the Causal Agent of Gray Mold

Metabolic activities of five botryticides against Botrytis cinerea examined using the Biolog FF MicroPlate

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Metabolic effects of azoxystrobin and kresoxim-methyl against Fusarium kyushuense examined using the Biolog FF MicroPlate

Cited by 24 publications

References 23 publications

Maize and Common Bean Seed Exudates Mediate Part of Nonhost Resistance to Phytophthora Sojae&nbsp;before Infection

Maize and Common Bean Seed Exudates Mediate Part of Nonhost Resistance to Phytophthora Sojae&nbsp;before Infection

Metabolic Phenotype Characterization of Botrytis cinerea, the Causal Agent of Gray Mold

Metabolic activities of five botryticides against Botrytis cinerea examined using the Biolog FF MicroPlate

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Maize and Common Bean Seed Exudates Mediate Part of Nonhost Resistance to Phytophthora Sojae before Infection

Maize and Common Bean Seed Exudates Mediate Part of Nonhost Resistance to Phytophthora Sojae before Infection