Metabolic effects and cellular volume responses induced by noradrenaline in nucleated erythrocytes

Kaloyianni, Martha; Giannisis, George; Gavriil, Panagiotis; Boukla, Anna

doi:10.1002/(sici)1097-010x(19971101)279:4<337::aid-jez3>3.0.co;2-p

Cited by 9 publications

(2 citation statements)

References 38 publications

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“…Specifically, adrenaline treatment of the digestive gland cells resulted in a significant increase of enzymatic activity as well an increase in pHi. The increases in pHi and PK activity were similar to β-adrenergic receptor activation in frog and human erythrocytes (Kaloyianni et al, 1997(Kaloyianni et al, , 2000Bourikas et al, 2003). The fact that cadmium, adrenaline and isoprenaline induce PK activation as well as increased pHi led us to investigate if cadmium also binds to β-adrenergic receptors.…”

Section: Discussionmentioning

confidence: 99%

Cadmium induces both pyruvate kinase and Na+/H+exchanger activity through protein kinase C mediated signal transduction, in isolated digestive gland cells ofMytilus galloprovincialis(L.)

Dailianis

Kaloyianni

2004

Journal of Experimental Biology

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SUMMARYThe present study investigates the transduction pathway mediated by cadmium in isolated digestive gland cells of mussel Mytilus galloprovincialis. The effects of cadmium treatment on a key glycolytic enzyme, pyruvate kinase (PK), and on Na+/H+ exchanger activity were examined. Cadmium (50 μmol l–1) caused a significant elevation of intracellular pH (pHi) and a rise (176%) of Na influx relative to control values. The amiloride analogue, EIPA (20 nmol l–1), a Na+/H+ exchanger blocker,together with cadmium, significantly reduced the effect of treatment by cadmium alone on both Na+ influx and pHi. In addition, PK activity was significantly increased after treatment with cadmium. PK activity was inhibited after treatment of cells with amiloride or EIPA together with cadmium. Moreover, phorbol-ester (PMA), a potent activator of protein kinase C(PKC), caused a significant rise in both pHi and PK activity, while staurosporine or calphostin C reversed both events. Adrenaline, isoprenaline and phenylephrine alone or together with cadmium also significantly increased the pHi and PK activity of isolated digestive gland cells. The latter effectors in combination with cadmium showed a synergistic effect on pHi and PK. These responses seem to be blocked by propranolol, metoprolol and prazosin. Our findings suggest a hormone-like effect of cadmium on digestive gland cells. The signal transduction pathway induced by cadmium involves the stimulation of PK, PKC and Na+/H+ exchanger in isolated digestive gland cells of Mytilus galloprovincialis.

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Section: Discussionmentioning

confidence: 99%

Cadmium induces both pyruvate kinase and Na+/H+exchanger activity through protein kinase C mediated signal transduction, in isolated digestive gland cells ofMytilus galloprovincialis(L.)

Dailianis

Kaloyianni

2004

Journal of Experimental Biology

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“…The influence of adrenergic stimulation on isotonic volume responses and ion fluxes has been examined in some amphibian RBCs, but responses were only significant in the presence of phosphodiesterase inhibitors; thus, the physiological significance of the responses remains unknown (Rudolph and Greengard, 1980;Tufts et al, 1987a;Tufts et al, 1987b;Kaloyianni et al, 1997).…”

Section: Introductionmentioning

confidence: 57%

Oxygen-sensitive regulatory volume increase and Na transport in red blood cells from the cane toad,Bufo marinus

Kristensen

Koldkjær

Berenbrink

et al. 2007

Journal of Experimental Biology

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SUMMARY The red blood cells (RBCs) of cane toad, Bufo marinus, are only partially saturated with oxygen in most of the circulation due to cardiac shunts that cause desaturation of arterial blood. The present study examines the oxygen dependency of RBC ouabain-insensitive unidirectional Na transport,using 22Na, in control cells and in cells exposed to hyperosmotic shrinkage or the β-adrenergic agonist isoproterenol. Deoxygenation per se induced a slow, but significant Na influx, which was paralleled by a slow increase in RBC volume. Hyperosmotic shrinkage by a calculated 25% activated a robust Na influx that in the first 30 min had a strong PO2 dependency with maximal activation at low PO2 values and a P50of ∼5.5 kPa. This activation was completely abolished by the Na/H exchanger (NHE) inhibitor EIPA (10–4 mol l-1). Hyperosmotic shrinkage is particularly interesting in B. marinus as it withstands considerable elevation in extracellular osmolarity following dehydration. Parallel studies showed that deoxygenated B. marinusRBCs had a much faster regulatory volume increase (RVI) response than air-equilibrated RBCs, reflecting the difference in magnitude of Na influxes at the two PO2 values. The extent of RVI(∼60%) after 90 min, however, was similar under the two conditions,reflecting a more prolonged elevation of the shrinkage-induced Na influx in air-equilibrated RBCs. There were no significant differences in the ability to perform RVI between whole blood cells at a PCO2of 1 and 3 kPa or washed RBCs, and 10–4 mol l-1amiloride reduced the RVI under all conditions, whereas 10–5mol l-1 bumetanide had no effect. Isoproterenol(10–5 mol l-1) induced a significant and prolonged increase in an EIPA-sensitive and bumetanide-insensitive Na influx at low PO2 under iso-osmotic conditions, whilst there was no stimulation by isoproterenol for up to 45 min in air-equilibrated RBCs. The prolonged β-adrenergic activation of the Na influx at low PO2 is distinctly different from the rapid and transient stimulation in teleost RBCs, suggesting significant differences in the signal transduction pathways leading to transporter activation between vertebrate groups.

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Regulation of cation transport pathways and glycolytic enzyme activity by alterations in red cell volume

Kaloyianni

Trachana

Boukla

1999

Cell Biochem. Funct.

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In the presence of NH4Cl and hypotonic solutions, Rana balcanica red cells respond by increasing their volume. The stimulation of cellular volume by hypotonicity is more rapid than that of NH4Cl, while the maximum value is less than that observed in the presence of NH4Cl. Depending on the cause of swelling, (nct uptake of NH4Cl or decrease in external osmolality) cells show specific responses. The NH4Cl treatment causes a significant increase in intracellular Na+, from 5.14 +/- 0.78 to 29.84 +/- 0.47 mmoles l-1 cell, while hypotonicity leads to a significant decrease of this cation, to 3.85 +/- 0.25 mmoles l-1 cell in relation to the control, after 30 min of incubation of Rana balcanica erythrocytes. In addition, amiloiride significantly reverses the NH4Cl effect with respect to intracellular Na+. Both treatments cause a significant K+ loss in comparison with controls. Two glycolytic enzymes glyceraldehyde phosphate dehydrogenase (GAPDH) and pyruvate kinase (PK) of Rana balcanica haemolysate were found to respond to the NH4Cl effect by significantly decreasing their activity.

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Metabolic effects and cellular volume responses induced by noradrenaline in nucleated erythrocytes

Cited by 9 publications

References 38 publications

Cadmium induces both pyruvate kinase and Na+/H+exchanger activity through protein kinase C mediated signal transduction, in isolated digestive gland cells ofMytilus galloprovincialis(L.)

Cadmium induces both pyruvate kinase and Na+/H+exchanger activity through protein kinase C mediated signal transduction, in isolated digestive gland cells ofMytilus galloprovincialis(L.)

Oxygen-sensitive regulatory volume increase and Na transport in red blood cells from the cane toad,Bufo marinus

Regulation of cation transport pathways and glycolytic enzyme activity by alterations in red cell volume

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