Metabolic Differences between Normal and Neoplastic Cells: Effects of Aminonucleoside on Cytoplasmic Messenger RNA

Cholon, Jolanta J.; Studzinski, George P.

doi:10.1126/science.184.4133.160

Cited by 17 publications

(8 citation statements)

References 17 publications

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“…The present studies indicate that there are differences in the composition and metabolism of nonhistone proteins of normal and SV40 transformed 3T3 mouse cells. Some of these differences appear to be consistent with those reported in association with normal and SV40 transformed WI38 human diploid fibroblasts (Zardi et al, 1973;Cholon et al, 1974;Lin et al, 1974;Krause et al, 1974Krause et al, , 1975a.…”

Section: Discussionsupporting

confidence: 87%

“…Several laboratories have recently reported complex alterations in the composition and metabolism of both histones and nonhistone chromosomal proteins of SV40 transformed WI38 human diploid fibroblasts (Zardi et al, 1973;Cholon et al, 1974;Linet al, 1974;Krause et al, 1974Krause et al, , 1975a. In the present studies we examined the chromosomal proteins of nor-mal and SV40 transformed 3T3 mouse cells to establish if any or all of the detected alterations might be a generalized feature of SV40 tranformation.…”

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confidence: 84%

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The effect of SV40 transformation on the chromosomal proteins of 3T3 mouse embryo fibroblasts

Krause

Noonan

Kleinsmith

et al. 1976

Cell Differentiation

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The composition and metabolism of chromosomal proteins-histones and nonhistones chromosomal proteins-were examined in normal and SV40 transformed 3T3 mouse cells. Variations were observed, many of which were similar to those previously reported for normal and SV40 transformed W138 human diploid fibroblasts. The possible implications of these viral induced changes in the protein component of the genome for the phenotypic modifications which occur in transformed cells are discussed.

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Section: Discussionsupporting

confidence: 87%

mentioning

confidence: 84%

The effect of SV40 transformation on the chromosomal proteins of 3T3 mouse embryo fibroblasts

Krause

Noonan

Kleinsmith

et al. 1976

Cell Differentiation

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“…by controlling the translation of the existing specific messenger RNA templates. We therefore studied the effect of cordycepin, an inhibitor of messenger RNA synthesis [8, [18][19][20], on the induction of HMG-CoA reductase. Although cordycepin inhibited messenger RNA synthesis in lymphocytes by more than 50% (cf.…”

Section: Discussionmentioning

confidence: 99%

Mechanism of regulation of 3‐hydroxy‐3‐methylglutaryl coenzyme A reductase activity by low density lipoprotein in human lymphocytes

Krone¹,

Betteridge²,

Galton³

1979

Eur J Clin Investigation

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Abstract. The mechanism of action of low density lipoprotein (LDL) on the rate of sterol synthesis and on the activity of its rate‐determining enzyme 3‐hydroxy‐3‐methylglutaryl coenzyme A reductase‐(HMG‐CoA reductase) was studied in lymphocytes freshly isolated from normal subjects and patients heterozygous for familial hypercholesterolaemia. Incubation of normal cells in lipid‐depleted serum led to a substantial rise in the rate of sterol synthesis from [14C]acetate. Addition of cycloheximide (20 μg/ml), a translational inhibitor, reduced sterol synthesis; addition of LDL (100 μg LDL‐cholesterol/ml) had a similar effect as cycloheximide in reducing sterol synthesis with a half‐life of about 3 h. Cordycepin (50 μg/ml) inhibited messenger RNA synthesis by more than 50% but had no inhibitory effect on the induction of sterol synthesis suggesting that the induction of the pathway is independent of newly synthesized messenger RNA. Lymphocytes from patients heterozygous for familial hypercholesterolaemia behaved similarly to cells from normal subjects in that cycloheximide reduced the incorporation of [14C]acetate into sterols, while cordycepin had no effect on the induction of sterol synthesis mediated by lipid‐depleted serum. Since sterol synthesis from [14C]acetate can be taken as a measure of HMG‐CoA reductase activity under our experimental conditions, the results suggest that: (i) the increase HMG‐CoA reductase activity in cells incubated in the presence of lipid‐depleted serum is due to increased de novo synthesis of the enzyme which can rapidly be inhibited by the addition of LDL, and (ii) in lymphocytes from normal subjects and patients heterozygous for familial hypercholesterolaemia the induction of HMG‐CoA reductase by lipid‐depleted serum and, by implication, the subsequent repression of the enzyme by LDL cannot be accounted for by a corresponding increase or decrease in the synthesis of messenger RNA.

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“…Complete inhibition of progress towards S is shown by the curve for cells left with low serum medium during the 9-hr interval; that is, the onset of DNA synthesis is delayed by [9][10] hr after the addition of serum. The drugs caffeine, PAN, and SVA all delayed progress towards S to an extent similar to that of low serum.…”

mentioning

confidence: 99%

Selective killing of transformed baby hamster kidney (BHK) cells.

Pardee¹,

James²

1975

Proc. Natl. Acad. Sci. U.S.A.

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We report here that certain drugs can protect Syrian baby hamster kidney cells (BHK) and 0.5 MiCi/ml for the desired time interval. Then they were rinsed with 2 ml of phosphate-buffered saline, and left for 1--8 hr with 1 ml of 5% trichloroacetic acid. The cells were dissolved in 0.8 ml of 2% Na2CO3 in 0.1 M NaOH. A 0.6 ml aliquot of this solution was added to 10 ml of Triton X-100-toluene-Spectrofluor scintillator and neutralized with 0.2 ml of 50% trichloroacetic acid. The radioactivity of samples was measured in an Intertechnique scintillation counter.

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Metabolic Differences between Normal and Neoplastic Cells: Effects of Aminonucleoside on Cytoplasmic Messenger RNA

Cited by 17 publications

References 17 publications

The effect of SV40 transformation on the chromosomal proteins of 3T3 mouse embryo fibroblasts

The effect of SV40 transformation on the chromosomal proteins of 3T3 mouse embryo fibroblasts

Mechanism of regulation of 3‐hydroxy‐3‐methylglutaryl coenzyme A reductase activity by low density lipoprotein in human lymphocytes

Selective killing of transformed baby hamster kidney (BHK) cells.

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