Metabolic Differences Between Hearts of Patients with Aortic Valve Disease and Hearts of Patients with Ischaemic Disease

Suleiman, M‐Saadeh; Caputo, Massimo; Ascione, Raimondo; Bryan, AJ; Lucchetti, Vincenzo; Gomes, Walter J.; Angelini, Gianni

doi:10.1006/jmcc.1998.0814

Cited by 21 publications

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“…Our earlier finding that the LV of patients with coronary artery disease had significantly higher alanine levels than patients with aortic valve stenosis (Suleiman et al 1998) has not been confirmed in this study. However, there was a strong tendency for alanine to be higher in both left and right ventricles of coronary artery disease hearts compared to aortic valve disease (Figure 1B).…”

Section: Discussioncontrasting

confidence: 89%

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Cardiac taurine and principal amino acids in right and left ventricles of patients with either aortic valve stenosis or coronary artery disease: the importance of diabetes and gender

et al. 2014

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Free intracellular taurine and principal α-amino acids (glutamate, glutamine, aspartate, asparagine and alanine) are abundant in human heart. They are cellular regulators and their concentration can change in response to disease and cardiac insults and have been shown to differ between hypertrophic left ventricle (LV) and the relatively "normal" right ventricle (RV) in patients with aortic valve stenosis (AVS). This difference has not been shown for coronary artery disease (CAD) and there are no studies that have simultaneously compared amino acid content in LV and RV from different pathologies. In this study we investigated the effect of disease on taurine and principal amino acids in both LV and RV, measured in myocardial biopsies collected from patients with either AVS (n = 22) or CAD (n = 36). Amino acids were extracted and measured using HPLC. Intra-and inter-group analysis was performed as well as subgroup analysis focusing on gender in AVS and type 2 diabetes in CAD. LV of both groups has significantly higher levels of taurine compared to RV. This difference disappears in both diabetic CAD patients and in male AVS patients. Alanine was the only α-amino acid to be altered by diabetes. LV of female AVS patients had significantly more glutamate, aspartate and asparagine than corresponding RV, whilst no difference was seen between LV and RV in males. LV of females has higher glutamate and glutamine and less metabolic stress than LV of males. This work shows that in contrast to LV, RV responds differently to disease which can be modulated by gender and diabetes.

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Section: Discussioncontrasting

confidence: 89%

“…As a result, the cardiac pump is likely to end up having two ventricles with different metabolic states. Left ventricular inter-disease (coronary vs. valve) differences in the content of amino acids have also been reported (Suleiman et al 1998). …”

Section: Introductionmentioning

confidence: 89%

Cardiac taurine and principal amino acids in right and left ventricles of patients with either aortic valve stenosis or coronary artery disease: the importance of diabetes and gender

et al. 2014

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“…In an earlier study we compared left ventricles of hearts with CAD with left ventricle from hearts with AVS and found that hypertrophic ventricle had significantly higher concentrations of ATP, but lower concentrations of lactate, branched-chain amino acids and alanine [34]. These differences have important implications for energy metabolism and protein turnover in the two pathologies.…”

Section: Discussionmentioning

confidence: 99%

The effect of disease on human cardiac protein expression profiles in paired samples from right and left ventricles

et al. 2014

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BackgroundCardiac diseases (e.g. coronary and valve) are associated with ventricular cellular remodeling. However, ventricular biopsies from left and right ventricles from patients with different pathologies are rare and thus little is known about disease-induced cellular remodeling in both sides of the heart and between different diseases. We hypothesized that the protein expression profiles between right and left ventricles of patients with aortic valve stenosis (AVS) and patients with coronary artery disease (CAD) are different and that the protein profile is different between the two diseases. Left and right ventricular biopsies were collected from patients with either CAD or AVS. The biopsies were processed for proteomic analysis using isobaric tandem mass tagging and analyzed by reverse phase nano-LC-MS/MS. Western blot for selected proteins showed strong correlation with proteomic analysis.ResultsProteomic analysis between ventricles of the same disease (intra-disease) and between ventricles of different diseases (inter-disease) identified more than 500 proteins detected in all relevant ventricular biopsies. Comparison between ventricles and disease state was focused on proteins with relatively high fold (±1.2 fold difference) and significant (P < 0.05) differences. Intra-disease protein expression differences between left and right ventricles were largely structural for AVS patients and largely signaling/metabolism for CAD. Proteins commonly associated with hypertrophy were also different in the AVS group but with lower fold difference. Inter-disease differences between left ventricles of AVS and CAD were detected in 9 proteins. However, inter-disease differences between the right ventricles of CAD and AVS patients were associated with differences in 73 proteins. The majority of proteins which had a significant difference in one ventricle compared to the other pathology also had a similar trend in the adjacent ventricle.ConclusionsThis work demonstrates for the first time that left and right ventricles have a different proteome and that the difference is dependent on the type of disease. Inter-disease differential expression was more prominent for right ventricles. The finding that a protein change in one ventricle was often associated with a similar trend in the adjacent ventricle for a large number of proteins suggests cross-talk proteome remodeling between adjacent ventricles.

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“…A maior espessura miocárdica da parede ventricular esquerda como também a elevação da pressão diastólica final limitam o fluxo coronariano subendocárdico, contribuindo para a inadequada distribuição da cardioplegia (21) . Também diferenças metabólicas importantes entre o miocárdio isquêmico e hipertrófico têm sido recentemente estabelecidas (22) . ORSINELLI et al (21) , em 1993, demonstraram que, quando associada a hipertrofia ventricular esquerda, a troca valvar aórtica apresenta mortalidade e morbidade aumentadas.…”

Section: Gráficounclassified

Efeitos das cardioplegias sangüíneas hipotérmica e normotérmica nos substratos intracelulares em pacientes com corações hipertróficos

Gomes¹,

Ascione²,

Suleiman³

et al. 2000

Rev Bras Cir Cardiovasc

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Efeitos das cardioplegias sangüíneas hipotérmica e normotérmica nos substratos intracelulares em pacientes com corações hipertróficos. Rev Bras Cir Cardiovasc 2000; 15(2): 160-8. RESUMO: Objetivos: As cardioplegias sangüíneas normotérmica e hipotérmica, administradas de maneira anterógrada e intermitente, têm demonstrado serem eficientes na proteção miocárdica em cirurgias de revascularização miocárdica. Entretanto, pouco se conhece da eficiência dessas técnicas em corações hipertróficos. Dessa maneira, foram estudados seus efeitos nos substratos intracelulares miocárdicos em pacientes portadores de estenose aórtica, submetidos a cirurgia de troca valvar aórtica.Casuística e Métodos: A concentração intracelular miocárdica dos substratos (ATP, lactato, glutamato, aspartato e alanina) foi medida em biópsias de ventrículo esquerdo de 20 pacientes submetidos a troca valvar aórtica, usando, como método de proteção miocárdica, cardioplegia sangüínea normotérmica (n=10) ou hipotérmica (n=10), administradas de forma anterógrada e intermitente. As biópsias foram retiradas 5 minutos após o início da circulação extracorpórea (controle), 30 minutos após o pinçamento aórtico (isquemia) e 20 minutos após o despinçamento (reperfusão).Resultados: Não houve alterações significantes na concentração intracelular dos substratos nas amostras coletadas durante o período isquêmico, em comparação ao controle. Na reperfusão, entretanto, houve significante queda nos valores de ATP e aminoácidos em ambos os grupos, em relação ao grupo controle.Conclusão: Os dados sugerem que ambos os protocolos de proteção miocárdica empregados não foram eficientes na proteção miocárdica de corações hipertróficos. DESCRITORES: Parada cardíaca induzida. Soluções cardioplégicas. Valva aórtica, cirurgia. Miocárdio, patologia. Miocárdio, fisiologia. Hipotermia induzida.Walter J. GOMES*, Raimondo ASCIONE**, M-Saadeh SULEIMAN**, Alan J. BRYAN**, Gianni D. ANGELINI** INTRODUÇÃOAs técnicas e estratégias de proteção miocárdica em cirurgia cardíaca têm sido profundamente alteradas nas últimas décadas. Essa evolução com contínuo aprimoramento tem sido possível através de intenso trabalho baseado em estudos de metabolismo aeróbio e anaeróbio do coração, cuidadosa observação em laboratório e subseqüente aplicação clínica. Lesões miocárdicas decorrentes da

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Metabolic Differences Between Hearts of Patients with Aortic Valve Disease and Hearts of Patients with Ischaemic Disease

Cited by 21 publications

References 0 publications

Cardiac taurine and principal amino acids in right and left ventricles of patients with either aortic valve stenosis or coronary artery disease: the importance of diabetes and gender

Cardiac taurine and principal amino acids in right and left ventricles of patients with either aortic valve stenosis or coronary artery disease: the importance of diabetes and gender

The effect of disease on human cardiac protein expression profiles in paired samples from right and left ventricles

Efeitos das cardioplegias sangüíneas hipotérmica e normotérmica nos substratos intracelulares em pacientes com corações hipertróficos

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