Metabolic correlation between polyol and energy-storing carbohydrate under osmotic and oxidative stress condition in Moniliella megachiliensis

Kobayashi, Yasuhiko; Iwata, Hisashi; Yoshida, Junjiro; Ogihara, Jun; Kasumi, Takafumi

doi:10.1016/j.jbiosc.2015.02.014

Cited by 26 publications

(10 citation statements)

References 43 publications

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“…The carbohydrate composition in fungal cells changes depending on the culture growth phase and physiological state. Thus, environmental challenges, such as thermal or osmotic shocks, and oxidative stress can promote either accumulation or consumption of certain carbohydrates, namely non-reducing disaccharide trehalose and two polyols, glycerol and D-arabitol, in a cell [7].…”

Section: Introductionmentioning

confidence: 99%

Soluble Sugar and Lipid Readjustments in the Yarrowia lipolytica Yeast at Various Temperatures and pH

et al. 2019

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Microorganisms cope with a wide range of environmental challenges using different mechanisms. Their ability to prosper at extreme ambient pH and high temperatures has been well reported, but the adaptation mechanism often remains unrevealed. In this study, we addressed the dynamics of lipid and sugar profiles upon different cultivation conditions. The results showed that the cells grown at various pH and optimal temperature contained mannitol as the major cytosol sugar alcohol. The elevated temperature of 38 °C led to a two- to three-fold increase in total cytosol sugars with concurrent substitution of mannitol for trehalose. Lipid composition in the cells at optimal temperature changed insignificantly at any pH tested. The increase in the temperature caused some drop in the storage and membrane lipid levels, remarkable changes in their composition, and the degree of unsaturated fatty acids. It was shown that the fatty acid composition of some membrane phospholipids varied considerably at changing pH and temperature values. The data showed a pivotal role and flexibility of the sugar and lipid composition of Y. lipolytica W29 in adaptation to unfavorable environmental conditions.

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Section: Introductionmentioning

confidence: 99%

Soluble Sugar and Lipid Readjustments in the Yarrowia lipolytica Yeast at Various Temperatures and pH

et al. 2019

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“…These results were similar to a previous report showing that RpoS regulates the expression of the carbon storage genes for the biosynthesis and degradation of glycogen and the osmoprotectant trehalose in Salmonella (Lévi-Meyrueis et al, 2014). Glycogen and trehalose are two important storage metabolites, and their amounts change in response to a number of environmental stresses, such as osmotic, oxidative, and cold stress (Dalmasso et al, 2012; Kobayashi et al, 2015). Additionally, the glycogen biosynthesis enzymes GlgC, GlgA, and GlgP have been observed to play key roles in the formation of biofilms in E .…”

Section: Discussionmentioning

confidence: 99%

Combined Transcriptome and Proteome Analysis of RpoS Regulon Reveals Its Role in Spoilage Potential of Pseudomonas fluorescens

Liu

Xu²,

Zhu

et al. 2019

Front. Microbiol.

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Microbial contamination is considered the main cause of food spoilage. Pseudomonas fluorescens is a typical spoilage bacterium contributing to a large extent to the spoilage process of proteinaceous foods. RpoS is known as an alternative sigma factor controlling stress resistance and virulence in many pathogens. Our previous work revealed that RpoS contributes to the spoilage activities of P. fluorescens by regulating resistance to different stress conditions, extracellular acylated homoserine lactone (AHL) levels, extracellular protease and total volatile basic nitrogen (TVB-N) production. However, RpoS-dependent genes in P. fluorescens remained undefined. RNA-seq transcriptomics analysis combined with quantitative proteomics analysis based on multiplexed isobaric tandem mass tag (TMT) labeling was performed in the P. fluorescens wild-type strain UK4 and its derivative carrying an rpoS mutation. A total of 375 differentially expressed coding sequences (DECs) and 212 differentially expressed proteins (DEPs) were identified. The DECs were further verified by qRT-PCR. The combined transcriptome and proteome analyses revealed the involvement of this regulator in several cellular processes, mainly including polysaccharide metabolism, intracellular secretion, extracellular structures, cell wall biogenesis, stress responses, and amino acid and biogenic amine metabolism, which may contribute to the biofilm formation, stress resistance, and spoilage activities of P. fluorescens. Moreover, we indeed observed that RpoS contributed to the production of the macrocolony biofilm's matrix. Our results provide insights into the regulatory network of RpoS and expand the knowledge about the role of RpoS in the functioning of P. fluorescens in food spoilage.

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“…On the other hand, those two polyols might be both important for Y. lipolytica osmotic balance and have roles that complement each other, as was observed in other yeasts. In studies on Moniliella megachiliensis, glycerol production was observed as a rapid response to osmotic stress, while erythritol was more associated with oxidative stress and the long-term osmotic stress response [36,37]. The yeast Trichosporonoides (Moniliella) oedocephalis secretes glycerol and erythritol simultaneously.…”

Section: Auxiliary Role To Hogmentioning

confidence: 99%

HOG-Independent Osmoprotection by Erythritol in Yeast Yarrowia lipolytica

Rzechonek

Szczepańczyk

Wang

et al. 2020

Genes

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Erythritol is a polyol produced by Yarrowia lipolytica under hyperosmotic stress. In this study, the osmo-sensitive strain Y. lipolytica yl-hog1Δ was subjected to stress, triggered by a high concentration of carbon sources. The strain thrived on 0.75 M erythritol medium, while the same concentrations of glucose and glycerol proved to be lethal. The addition of 0.1 M erythritol to the medium containing 0.75 M glucose or glycerol allowed the growth of yl-hog1Δ. Supplementation with other potential osmolytes such as mannitol or L-proline did not have a similar effect. To examine whether the osmoprotective effect might be related to erythritol accumulation, we deleted two genes involved in erythritol utilization, the transcription factor Euf1 and the enzyme erythritol dehydrogenase Eyd1. The strain eyd1Δ yl hog1Δ, which lacked the erythritol utilization enzyme, reacted to the erythritol supplementation significantly better than yl-hog1Δ. On the other hand, the strain euf1Δ yl-hog1Δ became insensitive to supplementation, and the addition of erythritol could no longer improve the growth of this strain in hyperosmotic conditions. This indicates that Euf1 regulates additional, still unknown genes involved in erythritol metabolism.

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Metabolic correlation between polyol and energy-storing carbohydrate under osmotic and oxidative stress condition in Moniliella megachiliensis

Cited by 26 publications

References 43 publications

Soluble Sugar and Lipid Readjustments in the Yarrowia lipolytica Yeast at Various Temperatures and pH

Soluble Sugar and Lipid Readjustments in the Yarrowia lipolytica Yeast at Various Temperatures and pH

Combined Transcriptome and Proteome Analysis of RpoS Regulon Reveals Its Role in Spoilage Potential of Pseudomonas fluorescens

HOG-Independent Osmoprotection by Erythritol in Yeast Yarrowia lipolytica

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