Metabolic clearance and secretion rates of subunits of human thyrotropin.

Kourides, I. A.; Ré, Richard N.; Weintraub, Bruce D.; Ridgway, E. Chester; Maloof, Farahe

doi:10.1172/jci108666

Cited by 44 publications

(11 citation statements)

References 33 publications

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“…Before SDS-PAGE, peak I counts were 30% immunoprecipitable, but counts eluted from gel slices after SDS-PAGE (i.e., "peak III"), were 72% immunoprecipitable, similar to the immunoprecipitability of native peak III seen in Fig. 7A (2,8,9). The severity and duration of the underlying thyroid disease are important, as illustrated by the significant correlation between serum T4 levels and PRL MCR (Fig.…”

Section: Resultssupporting

confidence: 58%

“…The MCR of growth hormone (GH), luteinizing hormone, follicle-stimulating hormone, thyrotropinstimulating hormone (TSH), and the subunits of TSH have been determined by this method (2)(3)(4)(5)(6)(7)(8)(9). Although the MCR of prolactini (PRL) has been imeasured in both the rat (10) ani-d sheep (11) in this mannier, comparable data in humanis have not been reported.…”

Section: Dopamine Infusion Decreased Prl Pr From 270 To 66mentioning

confidence: 98%

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Metabolic clearance and production rates of prolactin in man.

et al. 1979

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A B S T R A C T Metabolic clearance rates (MCR) and production rates (PR) of prolactin (PRL) have been determined by the constant infusion to equilibrium technique in 11 normal subjects, 6 patients with hyperthyroidism, 4 patients with hypothyroidism, and 9 patients with hyperprolactinemia. PRL MCR was also determined in four patients during dopamine infusion. Mean PRL MCR was 46±1 ml/min per m2 in women and 44±3 ml/min per m2 in men, and was significantly correlated with body mass (r = 0.84, P < 0.001). In contrast with controls, PRL MCR was higher in hyperthyroidism (MCR = 52±8 ml/min per mi2, P < 0.05), was slightly lower in hypothyroidism (MCR = 38+10 ml/min per m2, P = NS), and was significantly correlated with serum thyroxine (r = 0.46, P < 0.02). PRL MCR was lower than controls in hyperprolactinemia (MCR = 40+5 mllmin per i2, P <0.01) and was inversely correlated with serum PRL (r = -0.72, P < 0.001). PRL MCR was not significantly changed by dopamine infusion.Mean PRL PR for women and men was 211±74 and 187±44 ,ug/d per m2, respectively (P = NS). In hyperthyroidism the PRL PR was elevated (PR = 335±68 ,ug/d per m2, P < 0.02), but in hypothyroidism the increase (PR = 233±159 ,tg/d per m2) was not significant. In hyperprolactinemia the PRL PR was extremely high (PR = 31,000±29,000 ,ig/d per m2). Dopamine infusion decreased PRL PR from 270 to 66,ug/d per m2 indicating that its effect was on pituitary PRL secretion and not PRL metabolism.To evaluate possible circulating PRL heterogeneity that might arise during infusion, gel filtration of infusate and serum obtained during the MCR procedure was performed. Labeled monomeric PRL (peak III, Kav (partition coefficient) = 0.4) was partially A preliminary account of this work was presented at the 61st Annual Meeting of the Endocrine Society, Anaheim, Calif., 13-15 June, 1979. Received for publication 29 January 1979 and in revised form 9 August 1979. converted to two larger forms (peaks I and II) in vivo. Peak I (Kav = 0) was 30-40% immunoprecipitable, although peak II (Kav = 0.2) was not immunoprecipitable. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of peak I resulted in .90% conversion to peak III and restoration of full immunoactivity. Thus, peak

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Section: Resultssupporting

confidence: 58%

Section: Dopamine Infusion Decreased Prl Pr From 270 To 66mentioning

confidence: 98%

Metabolic clearance and production rates of prolactin in man.

et al. 1979

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“…Using (16)(17)(18)(19). This hypothesis was based upon the repeated observation that serum and intracellular levels of pituitary and placental free a-subunit exceed the serum and intracellular levels of intact glycoprotein hormones (14,15,40,41). In addition, in cell-free translation studies (13,21) and blot hybridization studies (42), a-subunit mRNA levels exceed LHfl-and TSHBsubunit mRNA levels in various physiologic situations.…”

Section: Discussionmentioning

confidence: 99%

Regulation of rat luteinizing hormone subunit messenger ribonucleic acids by gonadal steroid hormones.

Gharib¹,

Bowers²,

Need³

et al. 1986

J. Clin. Invest.

129

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Little is known about the hormonal regulation of luteinizing hormone (LH) biosynthesis. We have studied the regulation of LH messenger RNA (mRNA) levels by gonadal-steroid hormones in the rat. In one set of experiments, male and female rats were surgically gonadectomized (GDX) and killed 1, 3, 7, 14, 22, and 31 d postoperatively. In another set of experiments, male and female rats were surgically GDX and were injected subcutaneously with testosterone propionate (500 Mug/100 g body wt per d) or 17,8-estradiol 3-benzoate (10 lg/100 g body wt per d), respectively, beginning 3 wk postoperatively. Levels of serum LH were determined by radioimmunoassay and levels of LH subunit mRNAs in single pituitary glands were determined by blot hybridization analysis using labeled synthetic oligodeoxyribonucleotide probes that correspond to portions of the coding regions of the rat a-and LHfl-subunit mRNAs. 4 wk after gonadectomy, serum LH levels rose nine-and 20-fold, while a-subunit mRNA levels rose six-and 10-fold, and LHIf-subunit levels rose seven-and 14-fold, compared with controls in males and females, respectively. In gonadal-steroid hormone-treated male and female GDX rats, serum LH levels fell to 8 and 36% of control values, while a-subunit mRNA levels declined to 22 and 19%, and LH-f-subunit mRNA levels declined to 6 and 10% of control values, 48 h after injections were initiated, in males and females, respectively. We conclude that gonadal-steroid hormones negatively regulate the levels of both subunit mRNAs in GDX rats in a pattern that parallels the changes in serum LH values. These data suggest that gonadal-steroid hormone regulation of LH biosynthesis occurs, at least in part, at the level of LH subunit mRNAs due to effects at the transcriptional and/or RNA stability levels.

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“…The dissociated subunits are devoid of biological activity in vitro or in vivo and may be measured independently by radioimmunoassay. Both a and Iisubunits, in addition to complete TSH, are present in increased concentration in the serum of hypothyroid subjects and may be suppressed by administration of thyroid hormones or increased by TRH injection (Kourides et al 1973, Kourides, Re et al 1977. Evidence suggests that the subunits and whole molecule are synthesized and stored in the pituitary gland in which an excess of a subunit may usually be demonstrated.…”

Section: Glycoprotein Hormone -Thyroid Stimulating Hormone (Tsh)mentioning

confidence: 99%

Peripheral Metabolism of Hormones: Clinical Implications

Hoffenberg

1979

J R Soc Med

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Metabolic clearance and secretion rates of subunits of human thyrotropin.

Cited by 44 publications

References 33 publications

Metabolic clearance and production rates of prolactin in man.

Metabolic clearance and production rates of prolactin in man.

Regulation of rat luteinizing hormone subunit messenger ribonucleic acids by gonadal steroid hormones.

Peripheral Metabolism of Hormones: Clinical Implications

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