Metabolic Characterization of Dairy Cows Treated with Gossypol by Blood Biochemistry and Body Fluid Untargeted Metabolome Analyses

Tang, Chaohua; Zhang, Kai; Zhan, Tengfei; Zhao, Qingyu; Zhang, Junmin

doi:10.1021/acs.jafc.7b03544

Cited by 14 publications

(9 citation statements)

References 27 publications

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“…However, this difference is minuscule and without significant difference. Compared with the reference value, high plasma creatine kinase (CK) activity and lactate dehydrogenase may be associated with pregnancy, which was consistent with a previous study . In general, 21 indexes, such as total bilirubin (TBIL), alanine aminotransferase (ALT), and aspartate aminotransferase (AST), of serum biochemistry of GMO cows were detected and compared with those of nonGMO cows (Table ), which confirmed that the GMO cows were in normal physiological condition.…”

Section: Resultssupporting

confidence: 87%

Untargeted Metabonomics of Genetically Modified Cows Expressing Lactoferrin Based on Serum and Milk

Wang

Xie

et al. 2019

J. Agric. Food Chem.

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Metabolites of serum and milk from genetically modified (GM) cows and contrast check (CK) cows were comparatively investigated. Serum and milk were collected from genetically modified (GM) cows and contrast check (CK) cows, and then, they were analyzed using ultraperformance liquid chromatography–mass spectrometry (UPLC–MS) and gas chromatography–mass spectrometry (GC–MS). Although the level of some blood biochemical indexes for GM cows was shifted up or down, they were generally in normal physiological condition. Serum samples from lactoferrin GM cows exhibited reduced levels of amino acids and elevated levels of indoleacetate, α-keto acids, long-chain fatty acids, etc. GM milk possessed elevated levels of pentose and amino sugar metabolites, including arabitol, xylulose, glucuronate, and N-acetylgalactosamine. Interestingly, some essential nutrients, such as certain unsaturated fatty acids (e.g., eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and docosapentaenoic acid (DPA)), and some necessary rare sugars were significantly upregulated. Compared to the CK group, a Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was conducted based on the increased or decreased metabolites identified in the serum and milk samples of the GM group. The results showed that the GM cows were in healthy condition and their milk has improved benefits for customers. The milk from genetically modified cows was found to be a promising milk source for producing recombinant human lactoferrin (rhLF) for human beings.

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Section: Resultssupporting

confidence: 87%

Untargeted Metabonomics of Genetically Modified Cows Expressing Lactoferrin Based on Serum and Milk

Wang

Xie

et al. 2019

J. Agric. Food Chem.

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“…Higher volatile fatty acid concentration and milk composition yield were found in the HY group compared to the LY group. Recent studies suggested an important relationship between VFAs and milk components [ 2 , 30 , 31 ]. Specifically, of the three principle VFAs, acetate and butyrate are substrates for oxidation and are precursors of lipids [ 32 , 33 ], moreover propionate is the only glucogenic VFA, accounting for 65–80% of the net glucose supply in lactating dairy cows [ 34 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

Illumina sequencing analysis of the ruminal microbiota in high-yield and low-yield lactating dairy cows

et al. 2018

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In this study, differences in the ruminal bacterial community between high-yield and low-yield lactating dairy cows under the same dietary conditions were investigated. Sixteen lactating dairy cows with similar parity and days in milk were divided into high-yield (HY) and low-yield (LY) groups based on their milk yield. On day 21, rumen content samples were collected, and their microbiota compositions were determined using high-throughput sequencing of the 16S rRNA gene by the Illumina MiSeq platform. During the study period, dry matter intake (DMI) and milk yield were measured daily, and milk composition was assessed 3 times per week. The results showed that the milk of the LY group tended to have higher fat (P = 0.08), protein (P = 0.01) and total solid contents (P = 0.04) than that of the HY group, while the HY group had higher ruminal propionate (P = 0.08) proportion and volatile fatty acid (VFA) (P = 0.02) concentrations. Principal coordinate analysis indicated significant differences in ruminal bacterial community compositions and structures between the HY group and LY group. The abundances of Ruminococcus 2, Lachnospiraceae and Eubacterium coprostanoligenes were significantly higher in the HY group than in the LY group. In addition, Bacteroides, Ruminococcus 2 and Candidatus-Saccharimonas were positively correlated with ruminal propionate proportion (r>0.4, P<0.05). These findings enhance the understanding of bacterial synthesis within the rumen and reveal an important mechanism underlying differences in milk production in dairy cows.

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“…This finding suggests that due to host-microbiota interactions, different cows may harbour different microbial species compositions, which are closely related to distinct differences in rumen fermentation parameters and milk composition. Furthermore, recent findings suggest an important relationship between VFAs and milk components [2, 25, 26]. Specifically, of the three principle VFAs, acetic and butyric acids are substrates for oxidation and are precursors of lipids [27, 28].…”

Section: Discussionmentioning

confidence: 99%

Illumina sequencing analysis of the ruminal microbiota in high-yield and low-yield lactating dairy cows

Tong

Zhang

Yang

et al. 2018

Preprint

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9In this study, differences in the ruminal bacterial community between high-yield and 10 low-yield lactating dairy cows fed the same diets were investigated. Sixteen lactating 11 dairy cows with similar parity were divided into two groups based on their milk yield: 12 high-yield (HY) and low-yield (LY) groups. On day 21, rumen content samples were 13 collected, and the microbiota composition was determined using Illumina MiSeq 14 sequencing of the 16S rRNA gene. During the study period, dry matter intake (DMI) 15 and milk yield were measured daily, and milk composition was assessed 3 times per 16 week. The results showed that the milk of the LY group tended to have higher fat 17 (P=0.08), protein (P=0.01) and total solid (P=0.04) contents than that of the HY 18 group, though the HY group had higher ruminal acetate (P=0.05), propionate 19 (P=0.02) and volatile fatty acid (VFA) (P=0.02) concentrations. Principal coordinate 20 analysis indicated significant differences in ruminal bacterial community composition 21 and structure between the HY group and LY group. Overall, Bacteroidetes (HY 22 2 Corresponding author: kjxnb@vip.sina.com 28 LY group. These findings facilitate the understanding of bacterial synthesis within the 29 rumen and reveal an important mechanism underlying differences in milk production 30 in dairy cows. 31

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Metabolic Characterization of Dairy Cows Treated with Gossypol by Blood Biochemistry and Body Fluid Untargeted Metabolome Analyses

Cited by 14 publications

References 27 publications

Untargeted Metabonomics of Genetically Modified Cows Expressing Lactoferrin Based on Serum and Milk

Untargeted Metabonomics of Genetically Modified Cows Expressing Lactoferrin Based on Serum and Milk

Illumina sequencing analysis of the ruminal microbiota in high-yield and low-yield lactating dairy cows

Illumina sequencing analysis of the ruminal microbiota in high-yield and low-yield lactating dairy cows

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