Metabolic characterization and viable delivery of Akkermansia muciniphila for its future application

Ark, Kees van der

doi:10.18174/427507

Cited by 5 publications

(8 citation statements)

References 80 publications

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“…The addition of 0.1%, 0.5%, and 1% porcine bile extract resulted in increased growth of A. muciniphila in comparison to the medium that did not contain bile [ 46 ]. In contrast, purified bile salts addition of 0.5% or higher resulted in inhibited growth of A. muciniphila , whereas the addition of 0.1% purified bile salts did not inhibit growth [ 48 ]. Moreover, the survival of A. muciniphila in gastric juice was found to be very low [ 46 ].…”

Section: Physiologic Adaptation Of Akkermansia Muciniphimentioning

confidence: 99%

“…to be able to remain in bile, they are expected to harbor a mechanism for protection against bile. A putative bile acid transporter gene (Amuc_0139) is annotated in the genome of A. muciniphila , which might function to export bile acids from the cell [ 48 ]. This process could reduce the effects of bile acids inside the cells of A. muciniphila .…”

Section: Akkermansia Muciniphila Along the Humamentioning

confidence: 99%

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Akkermansia muciniphila in the Human Gastrointestinal Tract: When, Where, and How?

et al. 2018

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Akkermansia muciniphila is a mucin-degrading bacterium of the phylum Verrucomicrobia. Its abundance in the human intestinal tract is inversely correlated to several disease states. A. muciniphila resides in the mucus layer of the large intestine, where it is involved in maintaining intestinal integrity. We explore the presence of Akkermansia-like spp. based on its 16S rRNA sequence and metagenomic signatures in the human body so as to understand its colonization pattern in time and space. A. muciniphila signatures were detected in colonic samples as early as a few weeks after birth and likely could be maintained throughout life. The sites where Akkermansia-like sequences (including Verrucomicrobia phylum and/or Akkermansia spp. sequences found in the literature) were detected apart from the colon included human milk, the oral cavity, the pancreas, the biliary system, the small intestine, and the appendix. The function of Akkermansia-like spp. in these sites may differ from that in the mucosal layer of the colon. A. muciniphila present in the appendix or in human milk could play a role in the re-colonization of the colon or breast-fed infants, respectively. In conclusion, even though A. muciniphila is most abundantly present in the colon, the presence of Akkermansia-like spp. along the digestive tract indicates that this bacterium might have more functions than those currently known.

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Section: Physiologic Adaptation Of Akkermansia Muciniphimentioning

confidence: 99%

Section: Akkermansia Muciniphila Along the Humamentioning

confidence: 99%

Akkermansia muciniphila in the Human Gastrointestinal Tract: When, Where, and How?

et al. 2018

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“…1B). van der Ark also observed that A. muciniphila had a smaller size when growing in a basic mucin medium compared with those being cultivated in a soy protein medium 34 . A. muciniphila growing in the BM0.5 medium had smaller diameter than growing in the BHI-only medium (Fig.…”

Section: Resultsmentioning

confidence: 88%

“…The cell shape determining protein MreB (Amuc_0540) was downregulated by fourfolds in the BM0.5 group compared with BHI-only group. The mreB is a key gene regulating cell morphology and increasing cell length in A. muciniphila 34 .…”

Section: Resultsmentioning

confidence: 99%

Transcriptomics and metabolomics reveal the adaption of Akkermansia muciniphila to high mucin by regulating energy homeostasis

Fan

Liu

Xie

et al. 2021

Sci Rep

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In gut, Akkermansia muciniphila (A. muciniphila) probably exerts its probiotic activities by the positive modulation of mucus thickness and gut barrier integrity. However, the potential mechanisms between A. muciniphila and mucin balance have not been fully elucidated. In this study, we cultured the bacterium in a BHI medium containing 0% to 0.5% mucin, and transcriptome and gas chromatography mass spectrometry (GC–MS) analyses were performed. We found that 0.5% (m/v) mucin in a BHI medium induced 1191 microbial genes to be differentially expressed, and 49 metabolites to be changed. The metabolites of sorbose, mannose, 2,7-anhydro-β-sedoheptulose, fructose, phenylalanine, threonine, lysine, ornithine, asparagine, alanine and glutamic acid were decreased by 0.5% mucin, while the metabolites of leucine, valine and N-acetylneuraminic acid were increased. The association analysis between transcriptome and metabolome revealed that A. muciniphila gave strong responses to energy metabolism, amino sugar and nucleotide sugar metabolism, and galactose metabolism pathways to adapt to high mucin in the medium. This finding showed that only when mucin reached a certain concentration in a BHI medium, A. muciniphila could respond to the culture environment significantly at the level of genes and metabolites, and changed its metabolic characteristics by altering the effect on carbohydrates and amino acids.

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“…have also demonstrated that these metabolic pathways are intricately linked to processes of bacterial growth and cell morphology, as A. muciniphila requires exogenous GlcNAc or GalNAc for peptidoglycan synthesis 34,42 . Similar to our observation in sialidase-pretreated medium, this study also observed a highly elongated morphology of A. muciniphila when it was grown in synthetic PT medium 42 . The authors suggested that it could be caused by an imbalance between the availability of glucose and GlcNAc in this medium.…”

Section: Discussionmentioning

confidence: 99%

Growth inhibition of Akkermansia muciniphila by a secreted pathobiont sialidase

Muijlwijk

Bröring

Mierlo

et al. 2022

Preprint

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Akkermansia muciniphila is considered a key constituent of a healthy gut microbiota. In inflammatory bowel disease (IBD), A. muciniphila has a reduced abundance while other, putative pathogenic, mucus colonizers bloom. We hypothesized that interbacterial competition may contribute to this observation. By screening the supernatants of a panel of enteric bacteria, we discovered that a previously uncharacterized Allobaculum species potently inhibits the growth of A. muciniphila. Mass spectrometry analysis identified a secreted Allobaculum sialidase as inhibitor of A. muciniphila growth. The sialidase targets sialic acids on casein O-glycans, thereby altering the accessibility of nutrients critical for A. muciniphila. The altered glycometabolic niche results in distorted A. muciniphila cell division and efficiently arrests its growth. The identification of a novel mechanism of A. muciniphila growth inhibition by a competing bacterial pathobiont may provide a rationale for interventions aimed at restoring and maintaining a healthy microbiota symbiosis in patients with intestinal disease.

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Metabolic characterization and viable delivery of Akkermansia muciniphila for its future application

Cited by 5 publications

References 80 publications

Akkermansia muciniphila in the Human Gastrointestinal Tract: When, Where, and How?

Akkermansia muciniphila in the Human Gastrointestinal Tract: When, Where, and How?

Transcriptomics and metabolomics reveal the adaption of Akkermansia muciniphila to high mucin by regulating energy homeostasis

Growth inhibition of Akkermansia muciniphila by a secreted pathobiont sialidase

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