Metabolic changes in visual cortex of neonatal monocular enucleated rat: a proton magnetic resonance spectroscopy study

Chow, April M.; Zhou, Iris Y.; Fan, Shu Juan; Chan, Kannie W. Y.; Chan, Kevin C.; Wu, Ed X.

doi:10.1016/j.ijdevneu.2010.10.002

Cited by 23 publications

(34 citation statements)

References 86 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The brains' response to monocular enucleation in early postnatal life has also been tackled by in vivo Magnetic Resonance Spectroscopy (MRS; Table 1). Three weeks after surgery, Chow and colleagues (2011) found a significant reduction in the Taurine (Tau) and N-acetyl Aspartate (NAA) levels in the visual cortex contralateral to the enucleated eye indicative of neuronal loss and axonal damage (Chow, Zhou et al 2011). Glutamate (Glu), Choline (Cho) and myo-Inositol concentrations were not affected.…”

Section: The Impact Of Age On Neuroplasticitymentioning

confidence: 99%

Neuroplasticity and MRI: A perfect match

2016

View full text Add to dashboard Cite

Numerous studies have illustrated the benefits of physical workout and cognitive exercise on brain function and structure and, more importantly, on decelerating cognitive decline in old age and promoting functional rehabilitation following injury. Despite these behavioral observations, the exact mechanisms underlying these neuroplastic phenomena remain obscure. This gap illustrates the need for carefully designed in-depth studies using valid models and translational tools which allow to uncover the observed events up to the molecular level. We promote the use of in vivo magnetic resonance imaging (MRI) because it is a powerful translational imaging technique able to extract functional, structural, and biochemical information from the entire brain. Advanced processing techniques allow performing voxel-based analyses which are capable of detecting novel loci implicated in specific neuroplastic events beyond traditional regions-of-interest analyses. In addition, its non-invasive character sets it as currently the best global imaging tool for performing dynamic longitudinal studies on the same living subject, allowing thus exploring the effects of experience, training, treatment etc. in parallel to additional measures such as age, cognitive performance scores, hormone levels, and many others. The aim of this review is (i) to introduce how different animal models contributed to extend the knowledge on neuroplasticity in both health and disease, over different life stages and upon various experiences, and (ii) to illustrate how specific MRI techniques can be applied successfully to inform on the fundamental mechanisms underlying experience-dependent or activity-induced neuroplasticity including cognitive processes.

show abstract

Section: The Impact Of Age On Neuroplasticitymentioning

confidence: 99%

Neuroplasticity and MRI: A perfect match

2016

View full text Add to dashboard Cite

show abstract

“…In the visual system, recent studies on optic nerve injury and glaucoma indicated that application of brain-derived trophic factor to both the eye and visual cortex resulted in increased levels of retinal ganglion cell survival and function that exceeded those seen following treatment of the eye alone [170]. While the majority of studies on ocular diseases and optic neuropathies focused mainly on the anterior pathways, using localized single-voxel 1 H-MRS at 7 Tesla covering posterior visual brain nuclei, our recent studies indicated its feasibility in detecting alterations in major metabolites (e.g., Cho, glutamate, NAA, taurine, lactate and myo-inositol) in the steady state after complete or partial deafferentation of the anterior visual pathway with reference to creatine (Cr) level [58,[67][68][69]169] (Fig. 4).…”

Section: Evaluation Of Steady-state Metabolisms In Posterior Visual Nmentioning

confidence: 99%

“…In the longitudinal 1 H-MRS study, upon deafferentation of the adult hamster optic tract, a consistent increase in lactate, and a transient decrease in NAA and glutamate followed by a delayed increase in myoinositol were also found in the ipsilesional superior colliculus from 3 days to 4 weeks after lesion compared to the contralateral side [58] (c). (Images modified from [58,67,68]. )…”

Section: Evaluation Of Steady-state Metabolisms In Posterior Visual Nmentioning

confidence: 99%

“…In this paper, we review the recent use of high-field multiparametric magnetic resonance imaging and spectroscopic (MRI/MRS) methods (contrast-enhanced MRI, diffusion MRI, proton MRS and functional MRI) for in vivo and global assessments of the visual system from the anterior chamber [11,12], vitreous humor [11][12][13][14][15][16] and retina [10, in the eye to the optic nerve, optic chiasm and optic tract [28,29,, the superior colliculus (SC) [29,36,37,51,53,54,[57][58][59][60][61][62][63][64][65], the lateral geniculate nucleus (LGN) [29,54,61,[63][64][65] and the visual cortex [60,63,64,[66][67][68][69] of the brain in rodent studies. Despite a relatively low visual acuity, rodents are an excellent model for understanding the developmental and pathophysiological changes in the visual system, given their relatively simple structures, short lifespan, rapid growth and the ability to produce precisely defined changes in gene sequence [70][71][72][73]…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

IN VIVOMULTIPARAMETRIC MAGNETIC RESONANCE IMAGING AND SPECTROSCOPY OF RODENT VISUAL SYSTEM

Chan

Cheung²,

2010

J. Integr. Neurosci.

Self Cite

View full text Add to dashboard Cite

The integrity of the neuronal connections between eye and brain plays an important role in the performance of the mammalian visual system. However, the developmental and pathophysiological mechanisms in the visual system are largely unexplored due to the lack of a sensitive technique for directly assessing both anterior and posterior visual pathways longitudinally under the same experimental conditions. This paper reviewed the recent use of magnetic resonance imaging and spectroscopic (MRI/MRS) methods (contrast-enhanced MRI, diffusion MRI, proton MRS and functional MRI) at high magnetic field strengths, for in vivo and global assessments of the structure, metabolism and function of the visual system in normal, developing and injured rodent brains. Using animal models of ocular diseases, optic neuropathies, developmental plasticity and neonatal hypoxic-ischemic brain injury, focus is put on the feasibility of MRI/MRS to evaluate axonal transport and cellular activity along segregated fibers of the visual pathways, to characterize lesion-induced neurodegeneration in the retina and the optic nerve and tract, to detect steady-state metabolite changes in the posterior visual nuclei, and blood-ocular dynamic exchanges in the eye, and to understand the neurovascular coupling and functions in the retina and the visual brain nuclei. These studies suggested the significant values of high-field multiparametric MRI/MRS for providing early diagnoses and comprehensive therapeutic strategies for promoting functional recovery upon partial vision loss.

show abstract

“…Apart from contributing to visual neuroscience, enucleation as a type of deafferentation can be used to study the balance between neuroprotective 19 and neurodegenerative [20][21][22] properties of the central nervous system. Different procedures to perform enucleation are already described in literature.…”

Section: Introductionmentioning

confidence: 99%

A Highly Reproducible and Straightforward Method to Perform <em>In Vivo</em> Ocular Enucleation in the Mouse after Eye Opening

Aerts

Nys

Arckens

2014

JoVE

View full text Add to dashboard Cite

Enucleation or the surgical removal of an eye can generally be considered as a model for nerve deafferentation. It provides a valuable tool to study the different aspects of visual, cross-modal and developmental plasticity along the mammalian visual system [1][2][3][4] .Here, we demonstrate an elegant and straightforward technique for the removal of one or both eyes in the mouse, which is validated in mice of 20 days old up to adults. Briefly, a disinfected curved forceps is used to clamp the optic nerve behind the eye. Subsequently, circular movements are performed to constrict the optic nerve and remove the eyeball. The advantages of this technique are high reproducibility, minimal to no bleeding, rapid post-operative recovery and a very low learning threshold for the experimenter. Hence, a large amount of animals can be manipulated and processed with minimal amount of effort. The nature of the technique may induce slight damage to the retina during the procedure. This side effect makes this method less suitable as compared to Mahajan et al. (2011) 5 if the goal is to collect and analyze retinal tissue. Also, our method is limited to post-eye opening ages (mouse: P10 -13 onwards) since the eyeball needs to be displaced from the socket without removing the eyelids. The in vivo enucleation technique described in this manuscript has recently been successfully applied with minor modifications in rats and appears useful to study the afferent visual pathway of rodents in general.

show abstract

Metabolic changes in visual cortex of neonatal monocular enucleated rat: a proton magnetic resonance spectroscopy study

Cited by 23 publications

References 86 publications

Neuroplasticity and MRI: A perfect match

Neuroplasticity and MRI: A perfect match

IN VIVOMULTIPARAMETRIC MAGNETIC RESONANCE IMAGING AND SPECTROSCOPY OF RODENT VISUAL SYSTEM

A Highly Reproducible and Straightforward Method to Perform <em>In Vivo</em> Ocular Enucleation in the Mouse after Eye Opening

Contact Info

Product

Resources

About