Metabolic changes in undifferentiated and differentiated human colon adenocarcinoma cells studied by multinuclear magnetic resonance spectroscopy

Galons, Jean‐Philippe; Fantini, Jacques; Vion‐Dury, Jean; Cozzone, Patrick J.; Canioni, Paul

doi:10.1016/0300-9084(89)90077-1

Cited by 54 publications

(21 citation statements)

References 22 publications

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“…Together, these results demonstrate that a simultaneous activation of glycolytic and oxidative metabolism is necessary for efficient energy and biosynthetic substrate production from glucose. The present findings agree with previous reports that oxidative phosphorylation is increased to support high ATP demands during differentiation of various cell types, including human placental trophoblasts, nerve cells, and human colon adenocarcinoma cells [24][25][26].…”

Section: Discussionsupporting

confidence: 82%

Osteoclast Precursors Display Dynamic Metabolic Shifts toward Accelerated Glucose Metabolism at an Early Stage of RANKL-Stimulated Osteoclast Differentiation

Kim

Jeong

Kang

et al. 2007

Cell Physiol Biochem

112

116

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Mature osteoclasts have an increased citric acid cycle and mitochondrial respiration to generate high ATP production and ultimately lead to bone resorption. However, changes in metabolic pathways during osteoclast differentiation have not been fully illustrated. We report that glycolysis and oxidative phosphorylation characterized by glucose and oxygen consumption as well as lactate production were increased during receptor activator of nuclear factor-ĸB ligand (RANKL)-induced osteoclastogenesis from RAW264.7 and bone marrow-derived macrophage cells. Cell proliferation and differentiation varied according to glucose concentrations (0 to 100 mM). Maximal cell growth occurred at 20 mM glucose concentration and differentiation occurred at 5 mM concentration. Despite the similar growth rates exhibited when cultured cells were exposed to either 5 mM or 40 mM glucose, their differentiation was markedly decreased in high glucose concentrations. This finding suggests the possibility that osteoclastogenesis could be regulated by changes in metabolic substrate concentrations. To further address the effect of metabolic shift on osteoclastogenesis, we exposed cultured cells to pyruvate, which is capable of promoting mitochondrial respiration. Treatment of pyruvate synergistically increased osteoclastogenesis through the activation of RANKL-stimulated signals (ERK and JNK). We also found that osteoclastogenesis was retarded by blocking ATP production with either the inhibitors of mitochondrial complexes, such as rotenone and antimycin A, or the inhibitor of ATP synthase, oligomycin. Taken together, these results indicate that glucose metabolism during osteoclast differentiation is accelerated and that a metabolic shift towards mitochondrial respiration allows high ATP production and induces enhanced osteoclast differentiation.

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Section: Discussionsupporting

confidence: 82%

Osteoclast Precursors Display Dynamic Metabolic Shifts toward Accelerated Glucose Metabolism at an Early Stage of RANKL-Stimulated Osteoclast Differentiation

Kim

Jeong

Kang

et al. 2007

Cell Physiol Biochem

112

116

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“…Another possible explanation for the observed decrease in citrate associated cancer is related to a decrease in extracellular/ intracellular volume ratio (5,27,28). Unlike typical soft tissues (e.g., muscle and liver) there is a significant amount of extracellular space in prostatic tissues containing fluid which is extremely high in citrate (24 (27,28). This displacement of extracellular space and associated citrate rich prostatic fluids with cancer cells could also account for the observed decrease in citrate associated with prostatic cancer.…”

Section: Discussionmentioning

confidence: 99%

Citrate alterations in primary and metastatic human prostatic adenocarcinomas: ¹H magnetic resonance spectroscopy and biochemical study

Kurhanewicz

Dahiya

Macdonald

et al. 1993

Magnetic Resonance in Med

111

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The objectives of this study were to quantitatively verify the low levels of citrate previously observed in primary human prostatic adenocarcinomas and to determine whether citrate was further reduced in metastatic prostatic cancer. This was accomplished by comparison of citrate concentrations of DU 145 xenografts (a poorly differentiated human prostatic adenocarcinoma cell line grown in nude mice) with concentrations in primary human adenocarcinomas. Following in vivo 1H NMR studies of DU 145 xenografts, citrate concentrations of DU 145 xenografts and surgically removed primary prostatic adenocarcinoma tissue were determined by quantitative high resolution 1H NMR and enzymatic assay. The most significant findings of this study were that citrate concentrations in primary human adenocarcinomas (3.74 +/- 0.19 mumol/g wet weight) were significantly lower than those observed for normal and benign hyperplastic (BPH) prostatic tissues. Furthermore there was a further ten-fold reduction of citrate associated with DU 145 xenografts (0.31 +/- 0.028 mumole/g wet weight) compared with primary prostatic cancer. DU 145 xenografts also exhibited higher levels of uridine diphosphosugars and choline containing metabolites relative to primary prostatic adenocarcinomas. These findings support the hypothesis that citrate is low in primary prostatic cancer and further reduced in metastatic disease.

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“…Myo-inositol has been suggested to be a 'growth requirement' for mammalian cells or even simply the 'storage form' of the inositol polyphosphatide messenger system, because a significant amount of myo-inositol was demonstrated in the undifferentiated state of cells. 46 Free myo-inositol is an important component of both the central and peripheral nervous system where its concentration is almost one hundred-fold greater than that in plasma. 47,48 While the cellular localization of the myoinositol transport mechanism is not known, much of the free myo-inositol in peripheral nerve exists in Schwann cells, 49 and these cells have a relatively high affinity uptake mechanism for myo-inositol.…”

Section: Neurinomamentioning

confidence: 99%

Absolute concentrations of metabolites in human brain tumors usingin vitro proton magnetic resonance spectroscopy

1997

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Metabolic changes in undifferentiated and differentiated human colon adenocarcinoma cells studied by multinuclear magnetic resonance spectroscopy

Cited by 54 publications

References 22 publications

Osteoclast Precursors Display Dynamic Metabolic Shifts toward Accelerated Glucose Metabolism at an Early Stage of RANKL-Stimulated Osteoclast Differentiation

Osteoclast Precursors Display Dynamic Metabolic Shifts toward Accelerated Glucose Metabolism at an Early Stage of RANKL-Stimulated Osteoclast Differentiation

Citrate alterations in primary and metastatic human prostatic adenocarcinomas: ¹H magnetic resonance spectroscopy and biochemical study

Absolute concentrations of metabolites in human brain tumors usingin vitro proton magnetic resonance spectroscopy

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Metabolic changes in undifferentiated and differentiated human colon adenocarcinoma cells studied by multinuclear magnetic resonance spectroscopy

Cited by 54 publications

References 22 publications

Osteoclast Precursors Display Dynamic Metabolic Shifts toward Accelerated Glucose Metabolism at an Early Stage of RANKL-Stimulated Osteoclast Differentiation

Osteoclast Precursors Display Dynamic Metabolic Shifts toward Accelerated Glucose Metabolism at an Early Stage of RANKL-Stimulated Osteoclast Differentiation

Citrate alterations in primary and metastatic human prostatic adenocarcinomas: 1H magnetic resonance spectroscopy and biochemical study

Absolute concentrations of metabolites in human brain tumors usingin vitro proton magnetic resonance spectroscopy

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Citrate alterations in primary and metastatic human prostatic adenocarcinomas: ¹H magnetic resonance spectroscopy and biochemical study