Metabolic changes associated to the unblocking of adventitious root formation in aged, rooting-recalcitrant cuttings of Eucalyptus gunnii Hook. f. (Myrtaceae)

Battista, Federico Di; Maccario, Diego; Beruto, Margherita; Grauso, Laura; Lanzotti, Virginia; Curir, P.; Monroy, Fernando

doi:10.1007/s10725-019-00515-0

Cited by 13 publications

(4 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a comparative study of two olive cultivars of different rooting capacity, strong positive correlation was found between total flavonoid content and rooting percentage ( Denaxa et al, 2020 ). An increase in E. gunnii cutting flavonoid content has been associated with overcoming of AR recalcitrance ( Di Battista et al, 2019 ). In the present work, the flavonoid content parameter was virtually equally represented in the “species” and “time” factors.…”

Section: Discussionmentioning

confidence: 99%

Microcutting Redox Profile and Anatomy in Eucalyptus spp. With Distinct Adventitious Rooting Competence

et al. 2021

View full text Add to dashboard Cite

Adventitious root (AR) development takes place in an intricate cellular environment. Reactive oxygen species (ROS) and antioxidant defenses, triggered by wounding in cuttings, can modulate this process. A comparative assessment of biochemical and anatomical parameters at critical rooting stages in hard- (Eucalyptus globulus Labill.) and easy- (Eucalyptus grandis W.Hill ex Maiden) to-root species was carried out. Microcuttings from seedlings were inoculated in auxin-free AR induction medium and, after 96 h, transferred to AR formation medium for a period of 24 h. Samples were collected upon excision (Texc) and at the 5th day post excision (Tform). Delayed xylem development, with less lignification, was recorded in E. globulus, when compared to E. grandis, suggesting lower activity of the cambium layer, an important site for AR development. Superoxide was more densely present around the vascular cylinder at both sampled times, and in greater quantity in E. globulus than E. grandis, declining with time in the former. Hydrogen peroxide was localized primarily along cell walls, more intensely in the primary xylem and phloem, and increased significantly at Tform in E. globulus. Ascorbate peroxidase (APX), superoxide dismutase (SOD), and catalase (CAT) activities were generally higher in E. grandis and varied as a function of time in E. globulus. Soluble guaiacol peroxidase (GPRX) activity increased from Texc to Tform in both species, whereas cell wall-bound GPRX activity increased with time in E. grandis, surpassing E. globulus. Flavonoid content increased with time in E. grandis and was higher than E. globulus at Tform. Principal component analysis showed that species- and time-derived differences contributed to almost 80% of the variance. Overall, data indicate that E. grandis shows higher cambium activity and tighter modulation of redox conditions than E. globulus. These features may influence ROS-based signaling and phytohormone homeostasis of cuttings, thereby impacting on AR development. Besides being players in the realm of AR developmental differences, the specific features herein identified could become potential tools for early clone selection and AR modulation aiming at improved clonal propagation of this forest crop.

show abstract

Section: Discussionmentioning

confidence: 99%

Microcutting Redox Profile and Anatomy in Eucalyptus spp. With Distinct Adventitious Rooting Competence

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Furthermore, due to the strong recalcitrance character, E. nitens quickly decreases the adventitious rooting competence during ageing. This phenomenon is extended to other species, including E. gunii [ 21 ] and E. globulus [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 76%

Transcriptome Dynamics of Rooting Zone and Leaves during In Vitro Adventitious Root Formation in Eucalyptus nitens

Ayala

Acevedo

Luna

et al. 2022

Plants

View full text Add to dashboard Cite

Wood properties and agronomic traits associated with fast growth and frost tolerance make Eucalyptus nitens a valuable forest alternative. However, the rapid age-related decline in the adventitious root (AR) formation (herein, meaning induction, initiation, and expression stages) limits its propagation. We analyzed transcriptomic profile variation in leaves and stem bases during AR induction of microcuttings to elucidate the molecular mechanisms involved in AR formation. In addition, we quantified expressions of candidate genes associated with recalcitrance. We delimited the ontogenic phases of root formation using histological techniques and Scarecrow and Short-Root expression quantification for RNA sequencing sample collection. We quantified the gene expressions associated with root meristem formation, auxin biosynthesis, perception, signaling, conjugation, and cytokinin signaling in shoots harvested from 2- to 36-month-old plants. After IBA treatment, 702 transcripts changed their expressions. Several were involved in hormone homeostasis and the signaling pathways that determine cell dedifferentiation, leading to root meristem formation. In part, the age-related decline in the rooting capacity is attributable to the increase in the ARR1 gene expression, which negatively affects auxin homeostasis. The analysis of the transcriptomic variation in the leaves and rooting zones provided profuse information: (1) To elucidate the auxin metabolism; (2) to understand the hormonal and signaling processes involved; (3) to collect data associated with their recalcitrance.

show abstract

“…Even so, it is also di cult to root some woody plants, such as Eucalyptus gunnii Hook. f. (Myrtaceae) (Di Battista et al, 2019). We have shown that root predominant overexpression of the iaaM gene can drastically enhance root initiation from stem cuttings.…”

Section: Discussionmentioning

confidence: 97%

Root Predominant Overexpression of iaaM and CKX Genes Promotes Root Initiation and Biomass Production and Initiation in Citrus

Tang

Li²,

Zhai³

et al. 2023

Preprint

View full text Add to dashboard Cite

Promoting initiation and biomass production of roots is significant for plant-based industries including using roots as bioreactors. We used two citrus genotypes, Carrizo and US-897, as model plants to test the effects of root-predominantly overexpressed the iaaM (indoleacetic acid-tryptophan monooxygenase) gene and a CKX (a cytokinin oxidase/dehydrogenase) gene. The iaaM transgenic lines exhibited markedly faster root initiation, more root numbers, and higher root biomass compared to their wild-type counterparts. The transgenic iaaM + CKXplants also exhibited similar phenotypes, albeit to a lesser extent than the iaaMplants. Molecular analysis revealed an auxin-responsive CsGH3.1 gene was up-regulated in the iaaM roots and iaaM + CKX roots, and a cytokinin-responsive gene CsARR5 gene was down-regulated in the iaaM + CKX roots. Our results demonstrate that root predominant overexpression of the iaaM or both the iaaM and CKX genes drastically enhances the initiation, growth and biomass production of roots. These results provide additional support that manipulation of auxin and cytokinin levels in roots via transgenic or gene-editing technologies may benefit production of high-value secondary metabolites using roots as bioreactors and also improve rooting of recalcitrant plant species.

show abstract

Metabolic changes associated to the unblocking of adventitious root formation in aged, rooting-recalcitrant cuttings of Eucalyptus gunnii Hook. f. (Myrtaceae)

Cited by 13 publications

References 38 publications

Microcutting Redox Profile and Anatomy in Eucalyptus spp. With Distinct Adventitious Rooting Competence

Microcutting Redox Profile and Anatomy in Eucalyptus spp. With Distinct Adventitious Rooting Competence

Transcriptome Dynamics of Rooting Zone and Leaves during In Vitro Adventitious Root Formation in Eucalyptus nitens

Root Predominant Overexpression of iaaM and CKX Genes Promotes Root Initiation and Biomass Production and Initiation in Citrus

Contact Info

Product

Resources

About