Metabolic biomarkers for non-alcoholic fatty liver disease induced by high-fat diet: In vivo magnetic resonance spectroscopy of hyperpolarized [1-13C] pyruvate

Metabolic alterations in a rat model of hepatic ischaemia reperfusion injury: In vivo hyperpolarized 13C MRS and metabolic imaging

“…Given the limited sample size, future longitudinal studies involving more rats are needed to validate changes in [1‐ 13 C] pyruvate metabolism and IVIM‐DWI in the hepatic IRI model. Secondly, the quantification of the [1‐ 13 C] alanine and [1‐ 13 C] lactate was normalized to the [1‐ 13 C] pyruvate and tC signals, which is commonly used in the routine hyperpolarized 13 C studies . However, it is assumed that the increased ratios of these metabolites would be due to the decreased level of [1‐ 13 C] pyruvate resulting from the vasoconstriction after hepatic IRI.…”

“…[1‐ 13 C] pyruvic acid (30 mg; Isotec, Miamisburg, OH), containing 15 mmol/L of trityl radical (OX063; Oxford Instruments, Abingdon, UK) and 0.5 mmol/L Dotarem (Gd‐DOTA, Gurebet, France) was hyperpolarized using the Hypersense DNP polarizer (Oxford Instruments) with 94.1 GHz microwave in a 3.35 T field at 1.4 K for 1 hour . The hyperpolarized [1‐ 13 C] pyruvate was rapidly dissolved in 5 mL of a superheated alkaline buffer that consisted of 40 mmol/L Trizma preset crystals pH 7.6, 80 mmol/L NaOH, 50 mmol/L NaCl and 2.69 mmol/L disodium EDTA.…”

“…MR spectra were post‐processed and analysed using SAGE software (GE Healthcare) . Dynamic MRS data were apodized with a 10 Hz Gaussian filter in the time domain and zero filled to 16 384 points followed by Fourier transformation.…”

“…The metabolite peak heights were then corrected by fitting the dynamic time series of the hyperpolarized signal decay with a monoexponential function. The individual metabolites were normalized to the [1‐ 13 C] pyruvate and tC signals, which is the sum of the [1‐ 13 C] lactate, [1‐ 13 C] pyruvate‐hydrate, [1‐ 13 C] alanine and [1‐ 13 C] pyruvate signals . The differential metabolite ratios between the 2 groups were analysed by Wilcoxon signed‐rank test and Mann‐Whitney U test at P < .05 (two‐tailed test) using SPSS 19.0 (Chicago, IL, USA).…”

“…To our knowledge, although several hyperpolarized 13 C MRS studies have revealed the cellular metabolite changes in animal models with hepatic injuries, an in vivo hyperpolarized 13 C MRS and metabolic imaging study focusing on the effect of IRI for the early detection and diagnosis of impaired liver function, which are the vital steps in the identification of hepatic vascular complication and parenchymal abnormalities associated with hepatic disease has not been reported.…”