Metabolic behavior of denitrifying phosphate-accumulating organisms under nitrate and nitrite electron acceptor conditions

Ahn, Johwan; Daidou, Tomotaka; Tsuneda, Satoshi; Hirata, Akira

doi:10.1016/s1389-1723(01)80293-0

Cited by 56 publications

(18 citation statements)

References 18 publications

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“…Denitrifying PAOs have generally been defined to be specific PAOs capable of using oxygen or nitrite as well as nitrate for P uptake, and they are differentiated from PAOs with the ability to use only oxygen and nitrite for P uptake (8,11,12,21,44,(47)(48)(49)(50). In this study, the denitrifying P uptake properties of "Ca.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the Denitrification-Associated Phosphorus Uptake Properties of “Candidatus Accumulibacter phosphatis” Clades in Sludge Subjected to Enhanced Biological Phosphorus Removal

Kim

Lee

et al. 2013

Appl Environ Microbiol

119

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bTo characterize the denitrifying phosphorus (P) uptake properties of "Candidatus Accumulibacter phosphatis," a sequencing batch reactor (SBR) was operated with acetate. The SBR operation was gradually acclimated from anaerobic-oxic (AO) to anaerobic-anoxic-oxic (A2O) conditions by stepwise increases of nitrate concentration and the anoxic time. The communities of "Ca. Accumulibacter" and associated bacteria at the initial (AO) and final (A2O) stages were compared using 16S rRNA and polyphosphate kinase genes and using fluorescence in situ hybridization (FISH). The acclimation process led to a clear shift in the relative abundances of recognized "Ca. Accumulibacter" subpopulations from clades IIA > IA > IIF to clades IIC > IA > IIF, as well as to increases in the abundance of other associated bacteria (Dechloromonas [from 1.2% to 19.2%] and "Candidatus Competibacter phosphatis" [from 16.4% to 20.0%]), while the overall "Ca. Accumulibacter" abundance decreased (from 55.1% to 29.2%). A series of batch experiments combined with FISH/microautoradiography (MAR) analyses was performed to characterize the denitrifying P uptake properties of the "Ca. Accumulibacter" clades. In FISH/MAR experiments using slightly diluted sludge (ϳ0.5 g/liter), all "Ca. Accumulibacter" clades successfully took up phosphorus in the presence of nitrate. However, the "Ca. Accumulibacter" clades showed no P uptake in the presence of nitrate when the sludge was highly diluted (ϳ0.005 g/liter); under these conditions, reduction of nitrate to nitrite did not occur, whereas P uptake by "Ca. Accumulibacter" clades occurred when nitrite was added. These results suggest that the "Ca. Accumulibacter" cells lack nitrate reduction capabilities and that P uptake by "Ca. Accumulibacter" is dependent upon nitrite generated by associated nitrate-reducing bacteria such as Dechloromonas and "Ca. Competibacter."

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Section: Discussionmentioning

confidence: 99%

Characterization of the Denitrification-Associated Phosphorus Uptake Properties of “Candidatus Accumulibacter phosphatis” Clades in Sludge Subjected to Enhanced Biological Phosphorus Removal

Kim

Lee

et al. 2013

Appl Environ Microbiol

119

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“…Meinhold et al (1999) reported that a nitrite concentration of 8 mg N/L completely inhibited the anoxic phosphorus uptake by an EBPR sludge treating presettled domestic wastewater. In contrast, Ahn et al (2001) reported that the anoxic phosphorus uptake by an EBPR sludge continued to occur at a nitrite concentration of 40 mg N/L. Saito et al (2004) argued that the large difference in the above results was due to the use of different activated sludges and suggested that enriched PAO cultures should be used in order to get a better understanding of the inhibition.…”

Section: Introductionmentioning

confidence: 95%

“…Previous research has shown at least some PAOs, collectively called denitrifying PAOs (or DPAOs), are able to oxidize their intracellular PHA with nitrate and/or nitrite as the terminal electron acceptor, providing energy for phosphorus uptake (Ahn et al, 2001;Meinhold et al, 1999;Zeng et al, 2003). This means that the carbon source taken up by PAOs in the anaerobic phase is used for both denitrification and phosphorus removal, which is advantageous when the wastewater contains a relatively low level of organic carbon.…”

Section: Introductionmentioning

confidence: 99%

Free nitrous acid inhibition on anoxic phosphorus uptake and denitrification by poly‐phosphate accumulating organisms

Zhou

Pijuan

Yuan

2007

Biotech & Bioengineering

133

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Nitrite has been found in previous research an inhibitor on anoxic phosphorus uptake in enhanced biological phosphorus removal systems (EBPR). However, the inhibiting nitrite concentration reported varied in a large range. This study investigates the nitrite inhibition on anoxic phosphorus uptake by using four different mixed cultures performing EBPR with pH considered an important factor. The results showed that the protonated species of nitrite, HNO(2) (or free nitrous acid, FNA), rather than nitrite, is likely the actual inhibitor on the anoxic phosphorus uptake, as revealed by the much stronger correlation of the phosphorus uptake rate with the FNA than with the nitrite concentration. All the four EBPR sludges showed decreased anoxic phosphorus uptake rates with increased FNA concentrations in the studied range of 0.002-0.02 mg HNO(2)-N/L. The phosphorus uptake by all four cultures was completely inhibited at 0.02 mg HNO(2)-N/L. Granular sludge appeared to be more tolerant to HNO(2) than flocular sludge likely due to its stronger resistance to the transfer of nitrite into the bacterial aggregates. Furthermore, denitrification by the phosphorus-accumulating organisms (PAOs) was also found to be inhibited by HNO(2). The denitrification rate decreased by approximately 40% when the FNA concentration was increased from 0.002 to 0.02 mg HNO(2)-N/L.

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“…Actually, it has been reported that nitrite severely inhibits aerobic and anoxic phosphate uptake of Poly-Phosphate Accumulating Organisms (PAOs). However, the reported critical concentration of nitrite has been widely varying (Saito et al, 2004;Kuba et al, 1996;Lee et al, 2001;Meinhold et al, 1999;Ahn et al, 2001). In order to attain stable BPR performance, the mechanism of unstable response of PAOs to nitrite exposure must be clarified.…”

Section: Introductionmentioning

confidence: 99%

“…This is not curious, because some of PAOs can utilize nitrite as an electron acceptor under anoxic condition (Meinhold et al, 1999;Ahn et al, 2001;Hu et al, 2003;Shoji et al, 2003). Even though nitrite has an inhibitory effect, some of denitrifying PAOs must be able to utilize and detoxify nitrite under anoxic condition.…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of Unstable Nitrite Inhibition of Aerobic Phosphate Uptake

Saito

Takahashi

Tsuboi

et al. 2008

J. of Wat. & Envir. Tech.

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Recently, nitrite has been recognized as one of the considerable inhibitors of biological phosphorus removal. In fact, there are several reports on inhibitory effect of nitrite. While unfortunately, the reported critical levels of nitrite widely spread. So the real effect of nitrite has not yet been well understood. In this study, several batch tests were conducted to obtain stable and quantitative relation between the size of nitrite exposure and the size of inhibition. The obtained results are as follows; 1) Nitrite inhibits aerobic phosphate uptake of PAOs, but the inhibition is not direct inhibition by nitrite but indirect inhibition caused by reduced respiration, 2) PAOs with higher anoxic activity can reduce the inhibitory effect of nitrite, possibly because of aerobic nitrite denitrification, 3) Nitrite inhibition of aerobic phosphate uptake is successfully expressed by the model including aerobic nitrite denitrification rates. These results strongly suggest that unstable nitrite inhibition of aerobic phosphate uptake is caused by widely distributed anoxic activities of PAOs.

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Metabolic behavior of denitrifying phosphate-accumulating organisms under nitrate and nitrite electron acceptor conditions

Cited by 56 publications

References 18 publications

Characterization of the Denitrification-Associated Phosphorus Uptake Properties of “Candidatus Accumulibacter phosphatis” Clades in Sludge Subjected to Enhanced Biological Phosphorus Removal

Characterization of the Denitrification-Associated Phosphorus Uptake Properties of “Candidatus Accumulibacter phosphatis” Clades in Sludge Subjected to Enhanced Biological Phosphorus Removal

Free nitrous acid inhibition on anoxic phosphorus uptake and denitrification by poly‐phosphate accumulating organisms

Mechanisms of Unstable Nitrite Inhibition of Aerobic Phosphate Uptake

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