Metabolic Aspects of Spore Germination in Fungi

Allen, Paul

doi:10.1146/annurev.py.03.090165.001525

Cited by 96 publications

(41 citation statements)

References 73 publications

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“…Although these mechanisms may be important for surviving an extended drought, organisms that have physiologically transitioned into a dormant state must return to an active state at wet-up to compete for resources. The resuscitation process can be responsible for extremely large increases in respiration, as shown during spore germination in Bacillus megaterium (20) and many species of fungi (21). A high degree of gene coordination may be necessary for survival of water potential shock, as well as for entry into and resuscitation from dormant states.…”

Section: Birch Effectmentioning

confidence: 99%

Rainfall-induced carbon dioxide pulses result from sequential resuscitation of phylogenetically clustered microbial groups

Placella

Brodie

Firestone

2012

Proc. Natl. Acad. Sci. U.S.A.

410

359

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The pulse of carbon dioxide (CO 2 ) resulting from the first rainfall after the dry summer in Mediterranean ecosystems is so large that it is well documented at the landscape scale, with the CO 2 released in a few days comparable in magnitude to the annual net carbon exchange of many terrestrial ecosystems. Although the origin of this CO 2 is debated, we show that the pulse of CO 2 is produced by a three-step resuscitation of the indigenous microbial community. Specific phylogenetic groups of microorganisms activate and contribute to the CO 2 pulse at different times after a simulation of the first rainfall following the severe summer drought. Differential resuscitation was evident within 1 h of wet-up, with three primary response strategies apparent according to patterns of relative ribosomal quantity. Most bacteria could be classified as rapid responders (within 1 h of wet-up), intermediate responders (between 3 and 24 h after wet-up), or delayed responders (24–72 h after wet-up). Relative ribosomal quantities of rapid responders were as high in the prewet dry soils as at any other time, suggesting that specific groups of organisms may be poised to respond to the wet-up event, in that they preserve their capacity to synthesize proteins rapidly. Microbial response patterns displayed phylogenetic clustering and were primarily conserved at the subphylum level, suggesting that resuscitation strategies after wet-up of dry soil may be a phylogenetically conserved ecological trait.

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Section: Birch Effectmentioning

confidence: 99%

Rainfall-induced carbon dioxide pulses result from sequential resuscitation of phylogenetically clustered microbial groups

Placella

Brodie

Firestone

2012

Proc. Natl. Acad. Sci. U.S.A.

410

359

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“…Lipids are a prominent constituent of several species of fungi (Allen, 1965) and the activation or synthesis of an esterolytic system and its subsequent attack upon the lipid of the spore coat may be related to the swelling and increase in permeability of fungal spores which precedes germination (Yanagita, 1957 ;Ekundayo & Carlisle, 1964).…”

Section: Introductionmentioning

confidence: 99%

The Metabolism of Triglycerides by Spores of Penicillium roqueforti

Lawrence¹

1967

Journal of General Microbiology

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The ability of suspensions of washed spores of Penicillium roqueforti to oxidize triglycerides was markedly stimulated by L-proline, L-alanine and L-serine, although all the sources of nitrogen tested were effective over extended periods to different degrees. The rate of oxidation was further increased by the addition of certain sugars, although these were without effect in the absence of a nitrogen source. The same compounds that stimulated methylketone formation from triglycerides also promoted the rapid germination of spores, suggesting that common reactions were involved.The maximum yield of methylketone obtained was 25 yo from trioctanoin and trihexanoin at pH 6.0, with lower yields from tridecanoin, tributyrin and trilaurin. The addition of sodium azide, and to a lesser extent of 2,4-dinitrophenol and certain organophosphorus compounds, inhibited the oxidation of triglycerides. The possible role of fungal esterases in spore germination is discussed.

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“…As for the synthetic patterns of macromolecular synthesis, it is apparent that changes in the rates of RNA and protein accumulation are closely linked to cytological events, especially those marking the onset of outgrowth. The order of synthesis of these two components is the same as with many other fungal systems (Allen, 1965 ;Van Etten, 1969 ;Bainbridge, 1971) where RNA synthesis increased before protein synthesis. The pattern in S. pombe differs from the pattern displayed in the budding yeast S. cerevisiae during regrowth.…”

Section: Discussionmentioning

confidence: 67%

Germination and Outgrowth of Schizosaccharomyces pombe Spores Isolated by a Simple Batch Centrifugation Technique

Johnke

Padilla

1979

Journal of General Microbiology

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Spores of the fission yeast Schizosaccharomyces pombe have been separated from vegetative cells by a simple and rapid centrifugation (800 g for 20 min) through a 35 % Hypaque solution to a purity > 95 %. Approximately 35 yo of the spores were recovered. Regrowth in EMM2 plus glucose showed that over 97 yo of the spores germinated within the first 2 h and outgrowth continued between 5 and 10 h. Sucrose induced germination in > 95 % of the spores with a 1 h delay and outgrowth in 50 % of the spores with a 3 h delay. There was little protein synthesis during germination but the protein content increased linearly coincident with outgrowth. The RNA content increased slightly during germination, but increased linearly 1 h before the onset of outgrowth and protein synthesis. After 8 h of regrowth, coincident with the onset of DNA synthesis, the rate of RNA synthesis was accelerated. The DNA content had increased l.7-fold after 10 h of regrowth from a haploid level of 1.36 x ,ug spore-l.

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Metabolic Aspects of Spore Germination in Fungi

Cited by 96 publications

References 73 publications

Rainfall-induced carbon dioxide pulses result from sequential resuscitation of phylogenetically clustered microbial groups

Rainfall-induced carbon dioxide pulses result from sequential resuscitation of phylogenetically clustered microbial groups

The Metabolism of Triglycerides by Spores of Penicillium roqueforti

Germination and Outgrowth of Schizosaccharomyces pombe Spores Isolated by a Simple Batch Centrifugation Technique

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