Metabolic and transcriptional regulatory mechanism associated with postharvest fruit ripening and senescence in cherry tomatoes

Tang, Ning; An, Jing; Deng, Wei; Gao, Yongwen; Chen, Zexiong; Li, Zhengguo

doi:10.1016/j.postharvbio.2020.111274

Cited by 41 publications

(22 citation statements)

References 62 publications

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“…Cold storage of green or breaker tomato fruit down-regulates many pathways including those related to the ripening process (Rugkong et al, 2011;Zhang et al, 2019;Tang et al, 2020), but in the ripe fruit cold-affected transcriptome we detected two pathways that were strongly up-regulated. These were both related to protein metabolism: a proteasome pathway involved in protein degradation and a ribosome biogenesis pathway (Figure 7).…”

mentioning

confidence: 79%

“…If ripening is already advanced prior to cold storage, fruit are more resistant to chilling stress, display fewer chilling injury symptoms, and can be stored at lower temperatures and for longer periods than mature green or breaker fruit (Hobson, 1987;Gómez et al, 2009). Previous studies of transcriptome responses to chilling stress in tomato have used fruit harvested and stored at the mature-green or breaker stages, where ripening processes form a major part of transcriptional activity (Cruz-Mendívil et al, 2015;Albornoz et al, 2019;Zhang et al, 2019;Tang et al, 2020). The transcriptome responses to chilling stress of cherry tomato fruit that have already achieved full ripeness prior to cold storage are currently unknown.…”

Section: Introductionmentioning

confidence: 99%

“…Ripening in tomato involves changes in gene expression controlling accumulation of the carotenoid lycopene, alterations to sugar and organic acid composition, production of aroma volatiles, and changes to fruit firmness and textural properties. Chilling injury affects the gene expression of many of these aspects of the ripening program (Zhang et al, 2019;Tang et al, 2020), as well as the ethylene signaling and response pathway (Rugkong et al, 2011). Fruit softening during normal ripening is caused by reductions in cell turgor (Shackel et al, 1991) and by a range of modifications to the cell wall and middle lamella brought about by enzymes secreted from the symplast into the apoplast (Brummell and Harpster, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome Responses of Ripe Cherry Tomato Fruit Exposed to Chilling and Rewarming Identify Reversible and Irreversible Gene Expression Changes

et al. 2021

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Tomato fruit stored below 12°C lose quality and can develop chilling injury upon subsequent transfer to a shelf temperature of 20°C. The more severe symptoms of altered fruit softening, uneven ripening and susceptibility to rots can cause postharvest losses. We compared the effects of exposure to mild (10°C) and severe chilling (4°C) on the fruit quality and transcriptome of ‘Angelle’, a cherry-type tomato, harvested at the red ripe stage. Storage at 4°C (but not at 10°C) for 27 days plus an additional 6 days at 20°C caused accelerated softening and the development of mealiness, both of which are commonly related to cell wall metabolism. Transcriptome analysis using RNA-Seq identified a range of transcripts encoding enzymes putatively involved in cell wall disassembly whose expression was strongly down-regulated at both 10 and 4°C, suggesting that accelerated softening at 4°C was due to factors unrelated to cell wall disassembly, such as reductions in turgor. In fruit exposed to severe chilling, the reduced transcript abundances of genes related to cell wall modification were predominantly irreversible and only partially restored upon rewarming of the fruit. Within 1 day of exposure to 4°C, large increases occurred in the expression of alternative oxidase, superoxide dismutase and several glutathione S-transferases, enzymes that protect cell contents from oxidative damage. Numerous heat shock proteins and chaperonins also showed large increases in expression, with genes showing peak transcript accumulation after different times of chilling exposure. These changes in transcript abundance were not induced at 10°C, and were reversible upon transfer of the fruit from 4 to 20°C. The data show that genes involved in cell wall modification and cellular protection have differential sensitivity to chilling temperatures, and exhibit different capacities for recovery upon rewarming of the fruit.

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confidence: 79%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transcriptome Responses of Ripe Cherry Tomato Fruit Exposed to Chilling and Rewarming Identify Reversible and Irreversible Gene Expression Changes

et al. 2021

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“…The metabolite profiling is critical to discover biomarkers during the complex processes of fruit ripening and senescence. Various studies employ the metabolome and transcriptome tools to study the key genetic and molecular factors that affect and regulate fruit ripening and senescence, such as those in peach [ 42 ], tomato [ 43 , 44 ], pear [ 33 ], banana [ 45 ], mango [ 46 ], and other fruits. There are also limited reports on the study of metabolites in papaya.…”

Section: Discussionmentioning

confidence: 99%

Metabolomic and Transcriptomic Profiling Provide Novel Insights into Fruit Ripening and Ripening Disorder Caused by 1-MCP Treatments in Papaya

Zheng

Hao

Fan

et al. 2021

IJMS

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Treatment with 1-methylcyclopropylene (1-MCP) is an effective technique to preserve fruits, but inappropriate treatment with 1-MCP causes a ripening disorder (rubbery texture) in papaya fruit. In this study, a combined metabolomic and transcriptomic analysis was conducted to reveal the possible mechanism of the ripening disorder caused by unsuitable 1-MCP in papaya. A total of 203 differential accumulated metabolites (DAMs) were identified in the metabolome analysis. Only 24 DAMs were identified in the control (CK) vs. the 1-MCP 2 h group, and they were primarily flavonoids. Ninety and 89 DAMs were identified in the CK vs. 1-MCP 16 h and 1-MCP 2 h vs. 1-MCP 16 h groups, respectively, indicating that long-term 1-MCP treatment severely altered the metabolites during fruit ripening. 1-MCP 16 h treatment severely reduced the number of metabolites, which primarily consisted of flavonoids, lipids, phenolic acids, alkaloids, and organic acids. An integrated analysis of RNA-Seq and metabolomics showed that various energy metabolites for the tricarboxylic acid cycle were reduced by long-term treatment with 1-MCP, and the glycolic acid cycle was the most significantly affected, as well as the phenylpropane pathway. These results provide valuable information for fruit quality control and new insight into the ripening disorder caused by unsuitable treatment with 1-MCP in papaya.

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“…At the onset of tomato ripening, changes in primary metabolites are observed, such as the accumulation of glucose and fructose and the presence of citric and malic acids in ripe fruits [ 1 ]. Sugars and organic acids are critical to good flavor, contributing to sweetness and acid balance.…”

Section: Introductionmentioning

confidence: 99%

Post-Harvest Treatment with Methyl Jasmonate Impacts Lipid Metabolism in Tomato Pericarp (Solanum lycopersicum L. cv. Grape) at Different Ripening Stages

Meza

Tobaruela

Pascoal

et al. 2021

Foods

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The application of exogenous jasmonate can stimulate the production of ethylene, carotenoids, and aroma compounds and accelerate fruit ripening. These alterations improve fruit quality and make fruit desirable for human consumption. However, fruit over-ripening results in large losses of fruit crops. This problem is overcome by applying 1-methylcyclopropene to the fruits, due to its capacity to block the ethylene receptors, suppressing fruit ripening. In this study, treatments with only 1-methylcyclopropene and both 1-methylcyclopropene and methyl jasmonate were administered to observe whether exogenous methyl jasmonate can improve the metabolite levels in fruits with blocked ethylene receptors. Fruit pericarps were analyzed at 4, 10, and 21 days after harvest (DAH) and compared with untreated fruits. The post-harvest treatments affected primary metabolites (sugars, organic acids, amino acids, and fatty acids) and secondary metabolites (carotenoids, tocopherols, and phytosterols). However, the lipid metabolism of the tomatoes was most impacted by the exogenous jasmonate. Fatty acids, carotenoids, tocopherols, and phytosterols showed a delay in their production at 4 and 10 DAH. Conversely, at 21 DAH, these non-polar metabolites exhibited an important improvement in their accumulation.

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Metabolic and transcriptional regulatory mechanism associated with postharvest fruit ripening and senescence in cherry tomatoes

Cited by 41 publications

References 62 publications

Transcriptome Responses of Ripe Cherry Tomato Fruit Exposed to Chilling and Rewarming Identify Reversible and Irreversible Gene Expression Changes

Transcriptome Responses of Ripe Cherry Tomato Fruit Exposed to Chilling and Rewarming Identify Reversible and Irreversible Gene Expression Changes

Metabolomic and Transcriptomic Profiling Provide Novel Insights into Fruit Ripening and Ripening Disorder Caused by 1-MCP Treatments in Papaya

Post-Harvest Treatment with Methyl Jasmonate Impacts Lipid Metabolism in Tomato Pericarp (Solanum lycopersicum L. cv. Grape) at Different Ripening Stages

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