Metabolic and process engineering of Clostridium cellulovorans for biofuel production from cellulose

Yang, Xiaorui; Xu, Mengmeng; Yang, Shang‐Tian

doi:10.1016/j.ymben.2015.09.001

Cited by 124 publications

(69 citation statements)

References 72 publications

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“…has a great potential for value-added products production from cellulose (Sizova et al, 2011;Yang et al, 2015), while most of the reported studies using hemicellulose compounds of lignocellulosic biomass as a fermentation substrate have been focused on ethanol or hydrogen production rather than on butanol (Tolonen et al, 2011). However, few wild-type strains are known to produce butanol from cellulose or xylan via simultaneous saccharification and fermentation, leaving a need for development of one-step strategies for biobutanol production from lignocellulosic materials.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous saccharification and fermentation of hemicellulose to butanol by a non-sporulating Clostridium species

2016

Bioresource Technology

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h i g h l i g h t sSimultaneous saccharification and fermentation of xylan to butanol by new strain MF28. Simultaneous fermentation of glucose and xylose to produce 11.9 g/L butanol. High yields of butanol on xylan and xylooligosaccharide were obtained. No acetone/ethanol byproducts were formed in converting lignocellulose to butanol. Non-sporulating strain MF28 is capable of continuous industrial-scale fermentation. a r t i c l e i n f o t r a c tProduction of lignocellulosic butanol has drawn increasing attention. However, currently few microorganisms can produce biofuels, particularly butanol, from lignocellulosic biomass via simultaneous saccharification and fermentation. Here we report discovery of a wild-type, mesophilic Clostridium sp. strain MF28 that ferments xylan to produce butanol (up to 3.2 g/L) without the addition of saccharolytic enzymes and without any chemical pretreatments. Application of selective pressure from 2-deoxy-Dglucose facilitated isolation of strain MF28, which exhibits inactivation of genes (gid and ccp genes) responsible for carbon catabolite repression, thus allowing strain MF28 to simultaneously ferment a combination of glucose (30 g/L), xylose (15 g/L), and arabinose (15 g/L) to produce 11.9 g/L of butanol. Strain MF28 possesses several unique features: (i) non-sporulating, (ii) no acetone/ethanol, (iii) complete hemicellulose-binding enzymatic domain, and (iv) absence of carbon catabolite repression. These unique characteristics demonstrate the industrial potential of strain MF28 for cost-effective biofuel generation from lignocellulosic biomass.

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Section: Introductionmentioning

confidence: 99%

Simultaneous saccharification and fermentation of hemicellulose to butanol by a non-sporulating Clostridium species

2016

Bioresource Technology

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“…The low number of OTUs found in the LB-CMC medium was possibly due to the brief incubation time (48 h), the substrate complexity (higher than starch), the enrichment adaptation phase (lag phase) and the number of sequences obtained from the library. Using microorganisms with cellulase genes or a cellulosome complex sometimes requires 10 days only to break down 50% of the cellulose (Yang et al, 2015). Our comparison is also limited by the methodology used, focussed on obtaining starch-and cellulose-related microbial diversity of soil with sugarcane crops, which can explain the low rates of diversity indexes and the specificity of the observed OTUs ( Figure 6).…”

Section: Soil Physico-chemical Characteristicsmentioning

confidence: 99%

Starch- and cellulose-related microbial diversity of soil sown with sugarcane crops in the Papaloapan Basin, a megadiverse region of Mexico

López

Ruiz

Sánchez³

et al. 2018

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Introduction: Sugarcane is an essential agricultural product for bioethanol production in Mexico. The discovery of both the bacterial community associated with this crop and the soil status is a decisive step towards understanding how microorganisms influence crop productivity. Culture enrichment allows for the identification of the biodiversity of biological samples. The objective of this research was to identify the bacterial biodiversity related with two complex carbohydrate sources (starch and cellulose) in soils sown with sugarcane in the Papaloapan Basin in Oaxaca, Mexico via a metagenomic approach. Method: Soil content was analyzed chemically. Liquid LB, LB-starch and LB-1% carboximetilcellulose media were inoculated with 2 g soil and cultured at 180 rpm, 37°C for 48 h. The biomass was collected and the 16S rDNA gene was amplified and a library was constructed which was analyzed by sequencing.Results: N, K and Zn content of organic matter showed higher values than average, as opposed to P and Na, which were lower than average. In the library, 35 OTUs related to Clostridium, Bacillus, Enterococcus, Lysinibacillus and Citrobacter genera were found which could contain genes for breaking cellulose and starch.Discussion or Conclusion: This is the first approach to identify the diversity related to starch and cellulose hydrolysis in the Papaloapan region, where the principal genera detected were Clostridium, Bacillus, Enterococcus, Citrobacter and Lysinibacillus in a soil moderately rich in organic matter.

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“…Co-cultures or microbial consortia may also be utilized in the CBP systems as the third methodology. Brandon et al 2011Kang et al, 2010Kim et al, 2008Geddes et al, 2011Nieves et al, 2011Mullinnix, 2014Yang et al, 2014Jin et al, 2014 Jin et al 2012aOhgren et al 2006Wang et al, 2014aYasuda et al, 2014Zhu et al, 2014Fonseca et al, 2011Teixeira et al, 1999Jin et al, 2010Tang et al, 2011Zhang (J) et al, 2009Kim and Lee, 2005Zhang et al, 2012bYu et al, 2014Moreno et al, 2013Erdei et al, 2013aBallesteros et al, 2013Geddes et al, 2013Turhan et al, 2014Lan et al, 2013Hargreaves et al, 2013Alvira et al, 2011Olofsson et al, 2010a . In co-culture systems, saccharolytic and ethanologenic microorganisms are co-cultured to enhance efficient saccharification and fermentation in one pot.…”

Section: Strategies To Design Ideal Microorganisms For Cbpmentioning

confidence: 99%

Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review

2015

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HIGHLIGHTS Various CBP strategies have been discussed. Recently, lignocellulosic biomass as the most abundant renewable resource has been widely considered for bioalcohols production. However, the complex structure of lignocelluloses requires a multi-step process which is costly and time consuming. Although, several bioprocessing approaches have been developed for pretreatment, saccharification and fermentation, bioalcohols production from lignocelluloses is still limited because of the economic infeasibility of these technologies. This cost constraint could be overcome by designing and constructing robust cellulolytic and bioalcohols producing microbes and by using them in a consolidated bioprocessing (CBP) system. This paper comprehensively reviews potentials, recent advances and challenges faced in CBP systems for efficient bioalcohols (ethanol and butanol) production from lignocellulosic and starchy biomass. The CBP strategies include using native single strains with cellulytic and alcohol production activities, microbial co-cultures containing both cellulytic and ethanologenic microorganisms, and genetic engineering of cellulytic microorganisms to be alcohol-producing or alcohol producing microorganisms to be cellulytic. Moreover, high-throughput techniques, such as metagenomics, metatranscriptomics, next generation sequencing and synthetic biology developed to explore novel microorganisms and powerful enzymes with high activity, thermostability and pH stability are also discussed. Currently, the CBP technology is in its infant stage, and ideal microorganisms and/or conditions at industrial scale are yet to be introduced. So, it is essential to bring into attention all barriers faced and take advantage of all the experiences gained to achieve a high-yield and low-cost CBP process.

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Metabolic and process engineering of Clostridium cellulovorans for biofuel production from cellulose

Cited by 124 publications

References 72 publications

Simultaneous saccharification and fermentation of hemicellulose to butanol by a non-sporulating Clostridium species

Simultaneous saccharification and fermentation of hemicellulose to butanol by a non-sporulating Clostridium species

Starch- and cellulose-related microbial diversity of soil sown with sugarcane crops in the Papaloapan Basin, a megadiverse region of Mexico

Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review

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