Metabolic and immunological responses of male and female new Zealand Greenshell™ mussels (Perna canaliculus) infected with Vibrio sp.

Nguyen, Thao V.; Alfaro, Andrea C.; Mérien, Fabrice; Young, Tim; Grandiosa, Roffi

doi:10.1016/j.jip.2018.08.008

Cited by 58 publications

(26 citation statements)

References 86 publications

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“…Moreover, the accumulation of lactic acid and Krebs cycle intermediate (pyruvic acid) suggested that the Krebs cycle was disrupted by a switch towards anaerobic respiration. A similar result was also found in mussel P. canaliculus infected Vibrio sp (Nguyen et al, 2018b;Nguyen & Alfaro, 2019b) and surf clam Crassula aequilatera exposed to thermal stress (Alfaro, Nguyen & Mellow, 2019). When the ambient temperature exceeded the ABT, anaerobic metabolism in the limpet C. toreuma was enhanced via the opine pathway to provide energy (Han, Zhang & Dong, 2017).…”

Section: Amino Acid Metabolismsupporting

confidence: 70%

Metabolic response of Scapharca subcrenata to heat stress using GC/MS-based metabolomics

Jiang

Jiao

Sun

et al. 2020

PeerJ

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Marine mollusks are commonly subjected to heat stress. To evaluate the effects of heat stress on the physiological metabolism of the ark shell Scapharca subcrenata, clams were exposed to different high temperatures (24, 28 and 32 °C) for 72 h. The oxygen consumption and ammonia excretion rates were measured at 2, 12, 24, 48 and 72 h. The results indicated that the metabolic rates of the ark shell significantly increased with increasing heat stress, accompanied by mortalities in response to prolonged exposure. A metabolomics approach based on gas chromatography coupled with mass spectrometry was further applied to assess the changes of metabolites in the mantle of the ark shell at 32 °C. Moreover, multivariate and pathway analyses were conducted for the different metabolites. The results showed that the heat stress caused changes in energy metabolism, amino acid metabolism, osmotic regulation, carbohydrate metabolism and lipid metabolism through different metabolic pathways. These results are consistent with the significant changes of oxygen consumption rate and ammonia excretion rate. The present study contributes to the understanding of the impacts of heat stress on intertidal bivalves and elucidates the relationship between individual-level responses and underlying molecular metabolic dynamics.

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Section: Amino Acid Metabolismsupporting

confidence: 70%

Metabolic response of Scapharca subcrenata to heat stress using GC/MS-based metabolomics

Jiang

Jiao

Sun

et al. 2020

PeerJ

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show abstract

“…Moreover, the accumulation of lactic acid and Krebs cycle intermediate (pyruvic acid) suggested that the Krebs cycle was disrupted by a switch towards anaerobic respiration. A similar result was also found in mussel P. canaliculus infected Vibrio sp (Nguyen, 2018b;Nguyen & Alfaro, 2019b) and surf clam Crassula aequilatera exposed to thermal stress (Alfaro, Nguyen & Mellow, 2019). Manuscript to be reviewed When the ambient temperature exceeded the ABT, anaerobic metabolism in the limpet Cellana toreuma was enhanced via the opine pathway to provide energy (Han et al, 2016).…”

Section: Amino Acid Metabolismsupporting

confidence: 57%

Peer Review #1 of "Metabolic response of Scapharca subcrenata to heat stress using GC/MS-based metabolomics (v0.2)"

Nguyen¹

2020

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Marine mollusks are commonly subjected to heat stress. To evaluate the effects of heat stress on the physiological metabolism of the ark shell Scapharca subcrenata, clams were exposed to different high temperatures (24, 28, and 32 °C) for 72 h. The oxygen consumption and ammonia excretion rates were measured at 2, 12, 24, 48, and 72 h. The results indicated that the metabolic rates of the ark shell significantly increased with increasing heat stress, accompanied by mortalities in response to prolonged exposure. A metabolomics approach based on gas chromatography coupled with mass spectrometry was further applied to assess the changes of metabolites in the mantle of the ark shell at 32 °C. Moreover, multivariate and pathway analyses were conducted for the different metabolites. The results showed that the heat stress caused changes in energy metabolism, amino acid metabolism, osmotic regulation, carbohydrate metabolism, and lipid metabolism through different metabolic pathways. These results are consistent with the significant changes of oxygen consumption rate and ammonia excretion rate. The present study contributes to the understanding of the impacts of heat stress on intertidal bivalves and elucidates the relationship between individual-level responses and underlying molecular metabolic dynamics.

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“…Impacted metabolites in male mussels (e.g., threonine, glutamate, lysine, and histidine) suggest a change in energy metabolism, while a disturbance of both osmoregulation and energy metabolism is rather demonstrated in females (modulation of the osmolytes betaine and hypotaurine, ATP elevation). Other studies in environmental metabolomics also demonstrated the gender-specific response of mussels exposed to the pesticide triazophos [ 19 ], the flame retardant tetrabromobisphenol A [ 20 ], bisphenol A [ 21 ], and the pharmaceutical active compound fadrozole hydrochloride [ 22 ], or challenged by pathogens [ 23 , 24 ]. However, those studies did not fully take advantage of the multifactorial experimental structure of the data to characterize both exposure effects and gender-specific responses and to relate the observed variability (metabolic changes) to the main factors or their interaction in a multivariate context.…”

Section: Introductionmentioning

confidence: 99%

Multifactorial Analysis of Environmental Metabolomic Data in Ecotoxicology: Wild Marine Mussel Exposed to WWTP Effluent as a Case Study

et al. 2020

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Environmental metabolomics is a powerful approach to investigate the response of organisms to contaminant exposure at a molecular scale. However, metabolomic responses to realistic environmental conditions can be hindered by factors intrinsic to the environment and the organism. Hence, a well-designed experimental exposure associated with adequate statistical analysis could be helpful to better characterize and relate the observed variability to its different origins. In the current study, we applied a multifactorial experiment combined to Analysis of variance Multiblock Orthogonal Partial Least Squares (AMOPLS), to assess the metabolic response of wild marine mussels, Mytilus galloprovincialis, exposed to a wastewater treatment plant effluent, considering gender as an experimental factor. First, the total observed variability was decomposed to highlight the contribution of each effect related to the experimental factors. Both the exposure and the interaction gender × exposure had a statistically significant impact on the observed metabolic alteration. Then, these metabolic patterns were further characterized by analyzing the individual variable contributions to each effect. A main change in glycerophospholipid levels was highlighted in both males and females as a common response, possibly caused by oxidative stress, which could lead to reproductive disorders, whereas metabolic alterations in some polar lipids and kynurenine pathway were rather gender-specific. This may indicate a disturbance in the energy metabolism and immune system only in males. Finally, AMOPLS is a useful tool facilitating the interpretation of complex metabolomic data and is expected to have a broad application in the field of ecotoxicology.

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Metabolic and immunological responses of male and female new Zealand Greenshell™ mussels (Perna canaliculus) infected with Vibrio sp.

Cited by 58 publications

References 86 publications

Metabolic response of Scapharca subcrenata to heat stress using GC/MS-based metabolomics

Metabolic response of Scapharca subcrenata to heat stress using GC/MS-based metabolomics

Peer Review #1 of "Metabolic response of Scapharca subcrenata to heat stress using GC/MS-based metabolomics (v0.2)"

Multifactorial Analysis of Environmental Metabolomic Data in Ecotoxicology: Wild Marine Mussel Exposed to WWTP Effluent as a Case Study

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