Metabolic and genetic profiling of young adults with and without a family history of premature coronary heart disease (MAGNETIC). Study design and methodology

Abstract

Introduction First-degree relatives of individuals with premature coronary artery disease (CAD) are at increased risk of CAD. The research hypothesis of this project assumes that there are differences in the metabolic profiles between individuals with and without a positive family history (FH) of premature CAD. Material and methods The study group will comprise healthy patients ( n = 500) aged 18–35 years with a FH of premature CAD, and the control group ( n = 500) will consist of healthy subjects without a FH of premature CAD. Blood tests assessing the lipid profile will be carried out. Patients will respond to a questionnaire regarding FH and dietary habits. Measurement of carotid intima media thickness will be performed. Analysis of single-nucleotide polymorphisms (SNPs) associated with premature CAD will be carried out for every patient. Metabolomic profiling will be performed using a high-sensitivity Bruker AVANCE II 600 MHz NMR spectroscope. Results The results of this study will include a comparison of metabolic profiles assessed by 1H-NMR spectroscopy in the study and control groups and the results of analyses of the relationship between the metabolic profiles and genetic risk score calculated based on evaluated SNPs associated with premature CAD. Conclusions This study will deepen our knowledge of the aetiopathogenesis of atherosclerosis by identifying metabolic patterns associated with a positive FH of premature CAD. Obtaining a detailed FH will enable adjustments for major risk factors of premature CAD in the proband’s first-degree relatives. This research project also provides a chance to discover new biomarkers associated with the risk of premature CAD.

Family History of Premature Coronary Artery Disease (P-CAD)—A Non-Modifiable Risk Factor? Dietary Patterns of Young Healthy Offspring of P-CAD Patients: A Case-Control Study (MAGNETIC Project)

“…The following analysis is a part of the Metabolic and Genetic Profiling of Young Adults with and without a Family History of Premature Coronary Heart Disease (MAGNETIC) project, which is a case-control study that aims at analyzing classical and genetic risk factors of CAD in healthy young adults with and without a family history of P-CAD. Study design and methodology of the MAGNETIC project have been described previously [ 13 ]. In brief, the study sample was recruited between July 2015 and October 2017.…”

Metabolically healthy obese and metabolic syndrome of the lean: the importance of diet quality. Analysis of MAGNETIC cohort

“…Participants in this study were subjects from the MAG-NETIC (Metabolic and Genetic Profiling of Young Adults with and without a Family History of Premature Coronary Heart Disease) study. The design and methodology of the study have been described previously [25,26]. For the purpose of the current study, the sample was recruited between July 2015 and December 2017 ( Fig.…”

Novel inflammatory biomarkers may reflect subclinical inflammation in young healthy adults with obesity

“…The study group consisted of individuals aged 18-35 years from the MAGNETIC (Metabolic And GeNETic Profiling of Young Adults with and without a Family History of Premature Coronary Heart Disease) project, who were healthy descendants of patients hospitalised in the Silesian Centre for Heart Diseases because of premature coronary artery disease (myocardial infarction or angiographically confirmed stable coronary artery disease that occurred before age 55 years in men and before 65 years in women) [20,21]. The study flow chart is shown in Figure 1.…”