Metabolic and cardiovascular complications of HIV

Brennan-Benson, Paul

doi:10.1016/j.mpmed.2009.05.001

Cited by 1 publication

(1 citation statement)

References 16 publications

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“…During HIV infection, host metabolism is influenced among other things by the virus [1][2][3], immune responses [4][5][6] and treatment [7,8]. HAART, primarily used to restore HIV-induced effects, prolongs the lifespan of infected individuals [8] and has subsequently resulted in HIV infection becoming a chronic condition, especially in developed countries.…”

Section: Introductionmentioning

confidence: 99%

UPLC-MS Metabonomics Reveals Perturbed Metabolites in HIV-Infected Sera

Williams¹,

Kgoadi²,

Steffens³

et al. 2014

CMB

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Immune responses to infection by the human immunodeficiency virus (HIV) and the use of highly active antiretroviral therapy (HAART) to treat HIV infection, contributes to metabolic irregularities in the host.Current methods for the extraction and identification of metabolites in biofluids generally make use of laborious, time-consuming protocols. Here, 96-well Ostro™ plates and filtration under positive pressure was used to facilitate the simultaneous, reproducible extraction of metabolites from multiple serum samples which were then analyzed by ultra-performance liquid chromatography mass spectrometry (UPLC-MS). The easy to use solid phase extraction (SPE) protocol eliminated numerous potential contaminants while the UPLC-MS detection of metabolites produced visibly different chromatograms for HIV negative (n=16), HIV+ (n=13) and HIV+HAART+ (n=15) serum samples. Linear discriminant analysis (LDA) amplified these differences, classified the groups with 100% accuracy and identified biomarkers explaining the greatest variances between the groups. The 21 metabolites altered by HIV and/or HAART primarily represented those linked to lipid and energy pathways which is where known metabolic changes associated with HIV infection occur. This work demonstrated for the first time that Ostro TM plates and UPLC-MS metabonomics was able to successfully identify distinct differences between the experimental groups and detected metabolites related to HAART and other drugs used in the treatment of HIV-associated conditions. The findings of this approach suggests a possible role for this methodology in disease prognosis as well as in the monitoring of treatment success or failure and linking treatment to metabolic complications.

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