Metabolic and biochemical responses of Potamogeton anguillanus Koidz. (Potamogetonaceae) to low oxygen conditions

Parveen, Mahfuza; Miyagi, Atsuko; Kawai‐Yamada, Maki; Rashid, H.; Asaeda, Takashi

doi:10.1016/j.jplph.2018.11.023

Cited by 12 publications

(12 citation statements)

References 63 publications

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“…These metabolites may help researchers identify biomarkers for diagnosis and improve therapy through the design of target modulation drugs [6,7]. Metabolomics has already been widely used in biological studies of humans, animals, and plants [8,9]. Viruses are obligate parasites that hijack host cellular metabolism, limiting their growth and proliferation [10].…”

Section: Introductionmentioning

confidence: 99%

In Vitro and In Vivo Metabolomic Profiling after Infection with Virulent Newcastle Disease Virus

Liu

Yin

Gong

et al. 2019

Viruses

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Newcastle disease (ND) is an acute, febrile, highly contagious disease caused by the virulent Newcastle disease virus (vNDV). The disease causes serious economic losses to the poultry industry. However, the metabolic changes caused by vNDV infection remain unclear. The objective of this study was to determine the metabolomic profiling after infection with vNDV. DF-1 cells infected with the vNDV strain Herts/33 and the lungs from Herts/33-infected specific pathogen-free (SPF) chickens were analyzed via ultra-high-performance liquid chromatography/quadrupole time-of-flight tandem mass spectrometry (UHPLC-QTOF-MS) in combination with multivariate statistical analysis. A total of 305 metabolites were found to have changed significantly after Herts/33 infection, and most of them belong to the amino acid and nucleotide metabolic pathway. It is suggested that the increased pools of amino acids and nucleotides may benefit viral protein synthesis and genome amplification to promote NDV infection. Similar results were also confirmed in vivo. Identification of these metabolites will provide information to further understand the mechanism of vNDV replication and pathogenesis.

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Section: Introductionmentioning

confidence: 99%

In Vitro and In Vivo Metabolomic Profiling after Infection with Virulent Newcastle Disease Virus

Liu

Yin

Gong

et al. 2019

Viruses

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“…Further nontargeted or targeted quantitative profiling has resulted in a wide spectrum of data. The hypoxic/anoxic metabolomes of A. thaliana [27], Lotus japonicus [28], rice [29,30], soybean [31,32], wheat [33,34] as well as wetland species like Zostera marina [35], Potamogeton anguillanus [36], Epilobium hirsutum and E. palustre [37] have been studied (Table S1).…”

Section: Specificity Of Plant Metabolomic Profiles During Oxygen Lackmentioning

confidence: 99%

“…Now we will consider investigations provided on seagrasses, which are supposed to be even more tolerant to hypoxia. Oxygen deprivation of different intensities (hypoxia and anoxia) led to counter directional changes in the starch and soluble sugars in the shoots of pondweed P. anguillanus [36]. Quite different were the dynamics of glucose and fructose in eelgrass Z. marina [35].…”

Section: The Level Of Glucose Fructose and Other Carbohydratesmentioning

confidence: 99%

Plant Life with and without Oxygen: A Metabolomics Approach

Yemelyanov,

Puzanskiy,

Shishova

2023

IJMS

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Oxygen deficiency is an environmental challenge which affects plant growth, the development and distribution in land and aquatic ecosystems, as well as crop yield losses worldwide. The capacity to exist in the conditions of deficiency or the complete lack of oxygen depends on a number of anatomic, developmental and molecular adaptations. The lack of molecular oxygen leads to an inhibition of aerobic respiration, which causes energy starvation and the acceleration of glycolysis passing into fermentations. We focus on systemic metabolic alterations revealed with the different approaches of metabolomics. Oxygen deprivation stimulates the accumulation of glucose, pyruvate and lactate, indicating the acceleration of the sugar metabolism, glycolysis and lactic fermentation, respectively. Among the Krebs-cycle metabolites, only the succinate level increases. Amino acids related to glycolysis, including the phosphoglycerate family (Ser and Gly), shikimate family (Phe, Tyr and Trp) and pyruvate family (Ala, Leu and Val), are greatly elevated. Members of the Asp family (Asn, Lys, Met, Thr and Ile), as well as the Glu family (Glu, Pro, Arg and GABA), accumulate as well. These metabolites are important members of the metabolic signature of oxygen deficiency in plants, linking glycolysis with an altered Krebs cycle and allowing alternative pathways of NAD(P)H reoxidation to avoid the excessive accumulation of toxic fermentation products (lactate, acetaldehyde, ethanol). Reoxygenation induces the downregulation of the levels of major anaerobically induced metabolites, including lactate, succinate and amino acids, especially members of the pyruvate family (Ala, Leu and Val), Tyr and Glu family (GABA and Glu) and Asp family (Asn, Met, Thr and Ile). The metabolic profiles during native and environmental hypoxia are rather similar, consisting in the accumulation of fermentation products, succinate, fumarate and amino acids, particularly Ala, Gly and GABA. The most intriguing fact is that metabolic alterations during oxidative stress are very much similar, with plant response to oxygen deprivation but not to reoxygenation.

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“…Among ROS, the accumulation of H 2 O 2 is being widely adopted as a stress indicator in various plant stress studies, such as trees, shrubs, crops, and macrophytes ( Kuźniak and Urbanek, 2000 ; Mittler, 2002 ; Suzuki et al, 2014 ; Zhang et al, 2014 ; Niu and Liao, 2016 ; Parveen et al, 2019 ; Asaeda et al, 2020 ). Compared with other ROS, H 2 O 2 is relatively stable ( Sharma et al, 2012 ) and can be quantified chemically with minimum losses ( Satterfield and Bonnell, 1955 ).…”

Section: Introductionmentioning

confidence: 99%

Hydrogen Peroxide Variation Patterns as Abiotic Stress Responses of Egeria densa

et al. 2022

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In vegetation management, understanding the condition of submerged plants is usually based on long-term growth monitoring. Reactive oxygen species (ROS) accumulate in organelles under environmental stress and are highly likely to be indicators of a plant’s condition. However, this depends on the period of exposure to environmental stress, as environmental conditions are always changing in nature. Hydrogen peroxide (H2O2) is the most common ROS in organelles. The responses of submerged macrophytes, Egeria densa, to high light and iron (Fe) stressors were investigated by both laboratory experiments and natural river observation. Plants were incubated with combinations of 30–200 μmol m–2 s–1 of photosynthetically active radiation (PAR) intensity and 0–10 mg L–1 Fe concentration in the media. We have measured H2O2, photosynthetic pigment concentrations, chlorophyll a (Chl-a), chlorophyll b (Chl-b), carotenoid (CAR), Indole-3-acetic acid (IAA) concentrations of leaf tissues, the antioxidant activity of catalase (CAT), ascorbic peroxidase (APX), peroxidase (POD), the maximal quantum yield of PSII (Fv Fm–1), and the shoot growth rate (SGR). The H2O2 concentration gradually increased with Fe concentration in the media, except at very low concentrations and at an increased PAR intensity. However, with extremely high PAR or Fe concentrations, first the chlorophyll contents and then the H2O2 concentration prominently declined, followed by SGR, the maximal quantum yield of PSII (Fv Fm–1), and antioxidant activities. With an increasing Fe concentration in the substrate, the CAT and APX antioxidant levels decreased, which led to an increase in H2O2 accumulation in the plant tissues. Moreover, increased POD activity was proportionate to H2O2 accumulation, suggesting the low-Fe independent nature of POD. Diurnally, H2O2 concentration varies following the PAR variation. However, the CAT and APX antioxidant activities were delayed, which increased the H2O2 concentration level in the afternoon compared with the level in morning for the same PAR intensities. Similar trends were also obtained for the natural river samples where relatively low light intensity was preferable for growth. Together with our previous findings on macrophyte stress responses, these results indicate that H2O2 concentration is a good indicator of environmental stressors and could be used instead of long-term growth monitoring in macrophyte management.

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Metabolic and biochemical responses of Potamogeton anguillanus Koidz. (Potamogetonaceae) to low oxygen conditions

Cited by 12 publications

References 63 publications

In Vitro and In Vivo Metabolomic Profiling after Infection with Virulent Newcastle Disease Virus

In Vitro and In Vivo Metabolomic Profiling after Infection with Virulent Newcastle Disease Virus

Plant Life with and without Oxygen: A Metabolomics Approach

Hydrogen Peroxide Variation Patterns as Abiotic Stress Responses of Egeria densa

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