Metabolic Adjustment of Arabidopsis Root Suspension Cells During Adaptation to Salt Stress and Mitotic Stress Memory

Chun, Hyun Jin; Baek, Dongwon; Cho, Hyun Min; Jung, Hyun Suk; Jeong, Myeong Seon; Jung, Won Yong; Choi, Chang Min; Lee, Su Hyeon; Jin, Byung Jun; Park, Mi‐Suk; Kim, Hyun‐Jin; Chung, Woo Sik; Lee, Sang Yeol; Bohnert, Hans J.; Bressan, Ray A.; Yun, Dae‐Jin; Hong, Young-Shick; Kim, Min Chul

doi:10.1093/pcp/pcy231

Cited by 25 publications

(20 citation statements)

References 56 publications

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“…Our expressional and functional analyses of lignin biosynthesis genes in the responses of plants to salt stress have provided molecular, physiological, and genetic evidence supporting the importance of lignin biosynthesis in the cellular processes that endow plants with stress tolerance. Taken together, our previous metabolomics results of increased coniferin and lignin content in salt-adapted cells 8 and our present results suggest that enhanced lignin biosynthesis is a critical factor in plant adaptation and tolerance to salt stress. Furthermore, it is suggested that the three lignin units, i.e., G, H, and S monomers, may have different functions in plant adaptation and tolerance to salt stress.…”

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confidence: 89%

“…Previously, we reported that Arabidopsis root suspension cells allowed to adapt to high-salt conditions over time exhibited thicker cell walls and an increased lignin content when compared with normal cells, indicating that physical reinforcement of the root cell wall is an important component of the long-term salt stress adaptation in this species. 8 To investigate the molecular mechanisms underlying lignin biosynthesis in salt-adapted cells, we used quantitative real-time PCR to analyze the expression patterns of 15 genes involved in the lignin biosynthetic pathway (Figure 1a). 7,9 Notably, we observed significantly upregulated expression of CCoAOMT1, 4CL1, 4CL2, COMT1, PAL1, PAL2, and AtPrx52 in salt-adapted cells (A120) relative to normal cells (A0) (Figure 1a).…”

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confidence: 99%

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Lignin biosynthesis genes play critical roles in the adaptation of Arabidopsis plants to high-salt stress

Chun

Baek

Cho

et al. 2019

Plant Signaling & Behavior

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Salinity is a major abiotic stressor that limits the growth, development, and reproduction of plants. Our previous metabolic analysis of high salt-adapted callus suspension cell cultures from Arabidopsis roots indicated that physical reinforcement of the cell wall is an important step in adaptation to saline conditions. Compared to normal cells, salt-adapted cells exhibit an increased lignin content and thickened cell wall. In this study, we investigated not only the lignin biosynthesis gene expression patterns in salt-adapted cells, but also the effects of a loss-of-function of CCoAOMT1, which plays a critical role in the lignin biosynthesis pathway, on plant responses to high-salt stress. Quantitative realtime PCR analysis revealed higher mRNA levels of genes involved in lignin biosynthesis, including CCoAOMT1, 4CL1, 4CL2, COMT, PAL1, PAL2, and AtPrx52, in salt-adapted cells relative to normal cells, which suggests activation of the lignin biosynthesis pathway in salt-adapted cells. Moreover, plants harboring the CCoAOMT1 mutants, ccoaomt1-1 and ccoaomt1-2, were phenotypically hypersensitive to salt stress. Our study has provided molecular and genetic evidence indicating the importance of enhanced lignin accumulation in the plant cell wall during the responses to salt stress.

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confidence: 89%

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confidence: 99%

Lignin biosynthesis genes play critical roles in the adaptation of Arabidopsis plants to high-salt stress

Chun

Baek

Cho

et al. 2019

Plant Signaling & Behavior

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“…Our previous metabolite profiling study using salt-adapted Arabidopsis callus suspension-cultured cells reveals that various cellular processes, including cell wall thickening, play essential roles in plant salt adaptation [ 40 ]. In this proteomics study, we revealed that major differentially expressed proteins (DEPs) identified from salt-adapted cells were functionally associated with cytoskeleton and cell wall biogenesis.…”

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confidence: 99%

Microtubule Dynamics Plays a Vital Role in Plant Adaptation and Tolerance to Salt Stress

Chun

Baek

Jin

et al. 2021

IJMS

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Although recent studies suggest that the plant cytoskeleton is associated with plant stress responses, such as salt, cold, and drought, the molecular mechanism underlying microtubule function in plant salt stress response remains unclear. We performed a comparative proteomic analysis between control suspension-cultured cells (A0) and salt-adapted cells (A120) established from Arabidopsis root callus to investigate plant adaptation mechanisms to long-term salt stress. We identified 50 differentially expressed proteins (45 up- and 5 down-regulated proteins) in A120 cells compared with A0 cells. Gene ontology enrichment and protein network analyses indicated that differentially expressed proteins in A120 cells were strongly associated with cell structure-associated clusters, including cytoskeleton and cell wall biogenesis. Gene expression analysis revealed that expressions of cytoskeleton-related genes, such as FBA8, TUB3, TUB4, TUB7, TUB9, and ACT7, and a cell wall biogenesis-related gene, CCoAOMT1, were induced in salt-adapted A120 cells. Moreover, the loss-of-function mutant of Arabidopsis TUB9 gene, tub9, showed a hypersensitive phenotype to salt stress. Consistent overexpression of Arabidopsis TUB9 gene in rice transgenic plants enhanced tolerance to salt stress. Our results suggest that microtubules play crucial roles in plant adaptation and tolerance to salt stress. The modulation of microtubule-related gene expression can be an effective strategy for developing salt-tolerant crops.

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“…Considering metallic stress, in vitro culture systems focus on developing plant tolerance to chronic exposure to trace metals, contributing to the acquisition of an acclimation status. As a result of prolonged selection in the presence of stress agents, such as salinity or toxic elements, functioning of plant cells and regenerated plantlets is modified in comparison with non-exposed ones [ 19 , 61 , 62 ]. A hypothetical “priming agent” that induces acclimation to heavy metals in such culture systems has not been identified.…”

Section: Priming At Critical Growth Stagesmentioning

confidence: 99%

Priming Strategies for Benefiting Plant Performance under Toxic Trace Metal Exposure

Wiszniewska

2021

Plants

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Combating environmental stress related to the presence of toxic elements is one of the most important challenges in plant production. The majority of plant species suffer from developmental abnormalities caused by an exposure to toxic concentrations of metals and metalloids, mainly Al, As, Cd, Cu, Hg, Ni, Pb, and Zn. However, defense mechanisms are activated with diverse intensity and efficiency. Enhancement of defense potential can be achieved though exogenously applied treatments, resulting in a higher capability of surviving and developing under stress and become, at least temporarily, tolerant to stress factors. In this review, I present several already recognized as well as novel methods of the priming process called priming, resulting in the so-called “primed state” of the plant organism. Primed plants have a higher capability of surviving and developing under stress, and become, at least temporarily, tolerant to stress factors. In this review, several already recognized as well as novel methods of priming plants towards tolerance to metallic stress are discussed, with attention paid to similarities in priming mechanisms activated by the most versatile priming agents. This knowledge could contribute to the development of priming mixtures to counteract negative effects of multi-metallic and multi-abiotic stresses. Presentation of mechanisms is complemented with information on the genes regulated by priming towards metallic stress tolerance. Novel compounds and techniques that can be exploited in priming experiments are also summarized.

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Metabolic Adjustment of Arabidopsis Root Suspension Cells During Adaptation to Salt Stress and Mitotic Stress Memory

Cited by 25 publications

References 56 publications

Lignin biosynthesis genes play critical roles in the adaptation of Arabidopsis plants to high-salt stress

Lignin biosynthesis genes play critical roles in the adaptation of Arabidopsis plants to high-salt stress

Microtubule Dynamics Plays a Vital Role in Plant Adaptation and Tolerance to Salt Stress

Priming Strategies for Benefiting Plant Performance under Toxic Trace Metal Exposure

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