Metabolic adaptation of wheat grains contributes to a stable filling rate under heat stress

Wang, Xiaoming; Hou, Lijiang; Lu, Yunze; Wu, Bingjin; Gong, Xue; Liu, Manshuang; Wang, Jun; Sun, Qixin; Vierling, Elizabeth; Xu, Shengbao

doi:10.1093/jxb/ery303

Cited by 60 publications

(53 citation statements)

References 54 publications

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“…The growth, yield and quality of wheat are adversely affected by global climate changes, particularly increasing temperatures (heat stress, HS) (Tingley and Huybers, ; Lobell and Tebaldi, ; Tack et al ., ; Lesk et al ., ). Each degree Celsius increase in global mean temperature would reduce wheat yield by 6.0% (Zhao et al ., ), and this reduction is most severe during the grain‐filling stage (Wollenweber et al ., ; Farooq et al ., ; Tack et al ., ; Lesk et al ., ; Wang et al ., 2018a). A comprehensive understanding of the heat responses and adaptations, especially at the grain‐filling stage, is therefore urgently required to enable the development of practices to improve wheat thermotolerance.…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies have shown that the heat signal is probably transduced by several pathways to converge on heat shock factors (HSFs), followed by activation of a number of heat shock proteins (HSPs) and other heat‐responsive genes that drive the heat adaptation process in plants (Kotak et al ., ; Saidi et al ., ; Bokszczanin et al ., ). This heat adaptation confers thermotolerance on plant growth and development, and majorly contributes to maintaining the grain‐filling rate and the final yield of crops under HS (Wang et al ., 2018a). However, how plants sense HS and which pathways are involved in heat sensing and signaling to activate heat adaptation are largely unknown, hindering our full understanding of plant thermotolerance.…”

Section: Introductionmentioning

confidence: 99%

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Hybrid sequencing reveals insight into heat sensing and signaling of bread wheat

et al. 2019

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Wheat (Triticum aestivum L.), a globally important crop, is challenged by increasing temperatures (heat stress, HS). However its polyploid nature, the incompleteness of its genome sequences and annotation, the lack of comprehensive HS-responsive transcriptomes and the unexplored heat sensing and signaling of wheat hinder our full understanding of its adaptations to HS. The recently released genome sequences of wheat, as well as emerging single-molecular sequencing technologies, provide an opportunity to thoroughly investigate the molecular mechanisms of the wheat response to HS. We generated a high-resolution spatio-temporal transcriptome map of wheat flag leaves and filling grain under HS at 0 min, 5 min, 10 min, 30 min, 1 h and 4 h by combining full-length single-molecular sequencing and Illumina short reads sequencing. This hybrid sequencing newly discovered 4947 loci and 70 285 transcripts, generating the comprehensive and dynamic list of HS-responsive full-length transcripts and complementing the recently released wheat reference genome. Large-scale analysis revealed a global landscape of heat adaptations, uncovering unexpected rapid heat sensing and signaling, significant changes of more than half of HS-responsive genes within 30 min, heat shock factor-dependent and -independent heat signaling, and metabolic alterations in early HS-responses. Integrated analysis also demonstrated the differential responses and partitioned functions between organs and subgenomes, and suggested a differential pattern of transcriptional and alternative splicing regulation in the HS response. This study provided comprehensive data for dissecting molecular mechanisms of early HS responses in wheat and highlighted the genomic plasticity and evolutionary divergence of polyploidy wheat.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hybrid sequencing reveals insight into heat sensing and signaling of bread wheat

et al. 2019

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“…In wheat grains, comparative proteomic analysis has shown that water deficit and heat stress usually leads to decreases in protein synthesis/assembly components and metabolism-related proteins, but the stress/defense-related proteins and seed storage proteins are often increased, especially under heat stress [ 53 , 54 ]. Indeed, WS, HS, and WH treatments all led to significant increases in grain protein content (GPC) in DBA Aurora.…”

Section: Discussionmentioning

confidence: 99%

Multi-Omics Analysis of Small RNA, Transcriptome, and Degradome in T. turgidum—Regulatory Networks of Grain Development and Abiotic Stress Response

Liu

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2020

IJMS

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Crop reproduction is highly sensitive to water deficit and heat stress. The molecular networks of stress adaptation and grain development in tetraploid wheat (Triticum turgidum durum) are not well understood. Small RNAs (sRNAs) are important epigenetic regulators connecting the transcriptional and post-transcriptional regulatory networks. This study presents the first multi-omics analysis of the sRNAome, transcriptome, and degradome in T. turgidum developing grains, under single and combined water deficit and heat stress. We identified 690 microRNAs (miRNAs), with 84 being novel, from 118 sRNA libraries. Complete profiles of differentially expressed miRNAs (DEMs) specific to genotypes, stress types, and different reproductive time-points are provided. The first degradome sequencing report for developing durum grains discovered a significant number of new target genes regulated by miRNAs post-transcriptionally. Transcriptome sequencing profiled 53,146 T. turgidum genes, swith differentially expressed genes (DEGs) enriched in functional categories such as nutrient metabolism, cellular differentiation, transport, reproductive development, and hormone transduction pathways. miRNA–mRNA networks that affect grain characteristics such as starch synthesis and protein metabolism were constructed on the basis of integrated analysis of the three omics. This study provides a substantial amount of novel information on the post-transcriptional networks in T. turgidum grains, which will facilitate innovations for breeding programs aiming to improve crop resilience and grain quality.

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“…Moreover, heat stress affects the biosynthesis of secondary metabolites and biosynthesis of aminoacyl-tRNA [25]. The metabolic profiling of what grains using LC-MS/MS-HPLC technologies indicated higher concentrations of sucrose and G1p under heat stress [156]. Comparative metabolomics analysis was carried out for heat-tolerant and -susceptible soybean cultivars.…”

Section: Temperature Stress Regulationmentioning

confidence: 99%

Metabolomics: A Way Forward for Crop Improvement

et al. 2019

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Metabolomics is an emerging branch of “omics” and it involves identification and quantification of metabolites and chemical footprints of cellular regulatory processes in different biological species. The metabolome is the total metabolite pool in an organism, which can be measured to characterize genetic or environmental variations. Metabolomics plays a significant role in exploring environment–gene interactions, mutant characterization, phenotyping, identification of biomarkers, and drug discovery. Metabolomics is a promising approach to decipher various metabolic networks that are linked with biotic and abiotic stress tolerance in plants. In this context, metabolomics-assisted breeding enables efficient screening for yield and stress tolerance of crops at the metabolic level. Advanced metabolomics analytical tools, like non-destructive nuclear magnetic resonance spectroscopy (NMR), liquid chromatography mass-spectroscopy (LC-MS), gas chromatography-mass spectrometry (GC-MS), high performance liquid chromatography (HPLC), and direct flow injection (DFI) mass spectrometry, have sped up metabolic profiling. Presently, integrating metabolomics with post-genomics tools has enabled efficient dissection of genetic and phenotypic association in crop plants. This review provides insight into the state-of-the-art plant metabolomics tools for crop improvement. Here, we describe the workflow of plant metabolomics research focusing on the elucidation of biotic and abiotic stress tolerance mechanisms in plants. Furthermore, the potential of metabolomics-assisted breeding for crop improvement and its future applications in speed breeding are also discussed. Mention has also been made of possible bottlenecks and future prospects of plant metabolomics.

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Metabolic adaptation of wheat grains contributes to a stable filling rate under heat stress

Abstract: When wheat is subjected to heat stress during grain filling, metabolic adaptation reallocates resources to deposition of reserves and prioritizes a stable filling rate.

Cited by 60 publications

References 54 publications

Hybrid sequencing reveals insight into heat sensing and signaling of bread wheat

Hybrid sequencing reveals insight into heat sensing and signaling of bread wheat

Multi-Omics Analysis of Small RNA, Transcriptome, and Degradome in T. turgidum—Regulatory Networks of Grain Development and Abiotic Stress Response

Metabolomics: A Way Forward for Crop Improvement

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