Metabolic adaptation of diatoms to hypersalinity

Nikitashina, Vera; Stettin, Daniel; Pohnert, Georg

doi:10.1016/j.phytochem.2022.113267

Cited by 18 publications

(19 citation statements)

References 65 publications

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“…Threonic acid, a product of ascorbic acid oxidation, was found to accumulate in response to salt stress in Phaeodactylum tricornutum [ 99 ] and Thalassiosira pseudonana [ 100 ]. To the best of our knowledge, no effects of threonic acid intake were reported in dogs, but it depressed the ascorbic acid content of testes and liver in guinea pigs [ 101 ].…”

Section: Resultsmentioning

confidence: 99%

Nutritional Composition and Untargeted Metabolomics Reveal the Potential of Tetradesmus obliquus, Chlorella vulgaris and Nannochloropsis oceanica as Valuable Nutrient Sources for Dogs

Cabrita

Guilherme-Fernandes

Valente

et al. 2022

Animals

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The growing pet population is questioning the sustainability of the pet food system. Although microalgae may constitute a more sustainable food resource, the assessment of their potential for canine diets is almost non-existent. The present study aimed to evaluate the potential of three microalgae species (Tetradesmus obliquus, Chlorella vulgaris and Nannochloropsis oceanica) grown locally in industrial photobioreactors as alternative food resources for dogs. A detailed characterization of their nutritional composition and metabolomic profile was carried out and related to the nutritional requirements of dogs. Overall, the essential amino acid content exceeded the amounts required for dogs at all life stages, except methionine and cysteine. The three microalgae were deficient in linoleic acid, N. oceanica presented a linolenic acid content below requirements and T. obliquus and C. vulgaris were deficient in arachidonic and eicosapentaenoic acids. The fiber was mainly composed of insoluble dietary fiber. The mineral profile varied greatly with the microalgae species, demonstrating their different potential for dog feeding. Untargeted metabolomics highlighted glycolipids, glycerolipids and phospholipids as the most discriminating compounds between microalgae species. Overall, the results support the potential of T. obliquus, C. vulgaris and N. oceanica as valuable macro- and micro-nutrients sources for dog feeding.

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Section: Resultsmentioning

confidence: 99%

Nutritional Composition and Untargeted Metabolomics Reveal the Potential of Tetradesmus obliquus, Chlorella vulgaris and Nannochloropsis oceanica as Valuable Nutrient Sources for Dogs

Cabrita

Guilherme-Fernandes

Valente

et al. 2022

Animals

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“…The novel diatom exometabolites detected, and their enrichment in the exometabolome relative to the endometabolome, illustrate the importance of analyzing exudates, despite biases. For example, pyrrol‐2‐carboxylic acid was recently reported in the endometabolome of P. tricornutum and other diatoms and was found to increase in response to salt stress (Nikitashina et al ., 2022). We found that this metabolite, while detected in some cell pellet samples, was primarily an exometabolite.…”

Section: Discussionmentioning

confidence: 99%

Dynamic Phaeodactylum tricornutum exometabolites shape surrounding bacterial communities

et al. 2023

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Summary Roles of different ecological classes of algal exometabolites in regulating microbial community composition are not well understood. Here, we identify exometabolites from the model diatom Phaeodactylum tricornutum and demonstrate their potential to influence bacterial abundances. We profiled exometabolites across a time course of axenic algal growth using liquid chromatography–tandem mass spectrometry. We then investigated growth of 12 bacterial isolates on individual‐identified exometabolites. Lastly, we compared responses of a P. tricornutum‐adapted enrichment community to additions of two contrasting metabolites: selective growth substrate 4‐hydroxybenzoic acid and putative signaling/facilitator molecule lumichrome. We identified 50 P. tricornutum metabolites and found distinct temporal accumulation patterns. Two exometabolites (of 12 tested) supported growth of distinct subsets of bacterial isolates. While algal exudates and algal presence drove similar changes in community composition compared with controls, exogenous 4‐hydroxybenzoic acid addition promoted increased abundances of taxa that utilized it in isolation, and also revealed the importance of factors relating to algal presence in regulating community composition. This work demonstrates that secretion of selective bacterial growth substrates represents one mechanism by which algal exometabolites can influence bacterial community composition and illustrates how the algal exometabolome has the potential to modulate bacterial communities as a function of algal growth.

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“…Using the Liquid Chromatography-Tandem Mass Spectrometry (LC-MS 2 ) data, MAW annotated some of the known metabolites produced by S. marinoi , many of which were amino acids, analogues, and their derivatives such as betaine, pipecolic acid, 4-hydroxyproline, methionine sulfoxide, and N,N-dimethyl arginine. Such polar compounds alleviate under salt stress in S. marinoi and might act as osmoprotectants [35]. S. marinoi is also known to produce a rich portfolio of “Lipids and lipid-like molecules”.…”

Section: Discussionmentioning

confidence: 99%

Untargeted Metabolomics to Expand the Chemical Space of the Marine DiatomSkeletonema marinoi

Zulfiqar,

Stettin,

Schmidt

et al. 2023

Preprint

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Diatoms (Bacillariophyceae) are aquatic photosynthetic microalgae with an ecological role as primary producers in the aquatic food web. They account substantially for global carbon, nitrogen, and silicon cycling. Elucidating the chemical space of diatoms is crucial to understanding their physiology and ecology. To expand the known chemical space of a cosmopolitan marine diatom,Skeletonema marinoi, we performed High-Resolution Liquid Chromatography-Tandem Mass Spectrometry (LC-MS2) for untargeted metabolomics data acquisition. The spectral data from LC-MS2was used as input for the Metabolome Annotation Workflow (MAW) to obtain putative annotations for all measured features. A suspect list of metabolites previously identified in theSkeletonemaspp. was generated to verify the results. These known metabolites were then added to the putative candidate list from LC-MS2data to represent an expanded catalogue of 1970 metabolites estimated to be produced byS. marinoi. The most prevalent chemical superclasses, based on the ChemONT ontology in this expanded dataset, were “Organic acids and derivatives”, “Organoheterocyclic compounds”, “Lipids and lipid-like molecules”, and “Organic oxygen compounds”. The metabolic profile from this study can aid the bioprospecting of marine microalgae for medicine, biofuel production, agriculture, and environmental conservation. The proposed analysis can be applicable for assessing the chemical space of other microalgae, which can also provide molecular insights into the interaction between marine organisms and their role in the functioning of ecosystems.ImportanceDiatoms are abundant marine phytoplankton members and have great ecological importance and biochemical potential. The cosmopolitan diatomSkeletonema marinoihas become an ecological and environmental research model organism. In this study, we used untargeted metabolomics to acquire a general metabolic profile ofS. marinoito assess its chemical diversity and expand the known metabolites produced by this diatom.S. marinoiproduces a chemically diverse set of secondary metabolites with potential therapeutic properties, such as anti-cancer, antioxidant, and anti-inflammatory. Such metabolites are highly significant due to their potential role in drug discovery and bioeconomy. Lipids fromS. marinoialso have potential in the biofuel industry. Furthermore, the environmental fluctuations in the water bodies directly affect the production of different secondary metabolites from diatoms, which can be key indicators of climate change.

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Metabolic adaptation of diatoms to hypersalinity

Cited by 18 publications

References 65 publications

Nutritional Composition and Untargeted Metabolomics Reveal the Potential of Tetradesmus obliquus, Chlorella vulgaris and Nannochloropsis oceanica as Valuable Nutrient Sources for Dogs

Nutritional Composition and Untargeted Metabolomics Reveal the Potential of Tetradesmus obliquus, Chlorella vulgaris and Nannochloropsis oceanica as Valuable Nutrient Sources for Dogs

Dynamic Phaeodactylum tricornutum exometabolites shape surrounding bacterial communities

Untargeted Metabolomics to Expand the Chemical Space of the Marine DiatomSkeletonema marinoi

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