Metabolic adaptation following genome doubling in citrus doubled diploids revealed by non-targeted metabolomics

Tan, Feng-Quan; Tu, Hong; Wang, Rong; Wu, Xiaomeng; Xie, Kai‐Dong; Chen, Jiajing; Zhang, Hongyan; Xu, Juan; Guo, Wen‐Wu

doi:10.1007/s11306-017-1276-x

Cited by 33 publications

(19 citation statements)

References 52 publications

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“…Similarly, tetraploid genotypes of Solanum bulbocastanum displayed either similar or lower amounts of phenylpropanoids, tryptophan, tyrosine and α-chaconine compared to diploids (Caruso et al, 2013). Furthermore, non-targeted and targeted metabolic profiling of mature leaves from three doubled diploids of citrus and their diploid controls showed that primary metabolism takes priority over specialized metabolism in doubled diploid plants, since the levels of primary metabolites tended to increase and the levels of secondary metabolites tended to decrease (Tan et al, 2017). As can be observed, there is a lack of linearity between the increase in genetic material (ploidy level) and the production rate of specialized metabolites in different medicinal and aromatic plants (Iannicelli et al, 2020).…”

Section: Resultsmentioning

confidence: 98%

“…The wide distribution of tetraploids in common centaury implies that this genome status has an adaptive significance and that it is selected positively. Secondary metabolites are often produced to protect plants against biotic and abiotic stresses and require a high investment in energy and carbon (Tan et al, 2017). Taking into account that C. erythraea plants within the present study were grown under experimental in vitro conditions where 2x and 4x genotypes were exposed to the same environment, the differences in specialized metabolites production may be attributed to genetic differences between the two groups of samples, and to their metabolic capacity under these specific conditions.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, manipulation with ploidy, as a biotechnological tool, could be useful in improving pharmaceutical value of medicinal plants, since synthetic autopolyploids usually exhibit increases in bioactive metabolite concentration compared to their diploid progenitors (Javadian et al, 2017;Kong et al, 2017;Lavania et al, 2012;Sadat Noori et al, 2017;Tavan et al, 2015;Xu et al, 2014). However, several studies have shown that polyploidization did not affect the metabolite production (Hull-Sanders et al, 2009;Wohlmuth et al, 2005) or even that the effect of polyploidy on the production of specialized metabolites was negative (Caruso et al, 2013;Cohen et al, 2013;Mansouri and Bagheri, 2017;Tan et al, 2017). Furthermore, in comparison with diploids, polyploids generally produce larger cells which can result in an increased size of plant structures such as leaves, flowers and fruit, increased biomass and often show greater resistance to biotic and abiotic stress (Lavania et al, 2012;Yildiz, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Diploid vs. tetraploid Centaurium erythraea Rafn: A comparative study of regenerative in vitro potential and biosynthetic capacity

Filipović¹,

Šiler²,

Nestorović-Živković³

et al. 2019

Lekovite sirovine

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The impact of ploidy level on both the regenerative potential under in vitro conditions and the production of major bioactive specialized metabolites, such as iridoids and xanthones, was examined in Centaurium erythraea Rafn. Shoot regeneration frequency was genotype dependent, but not affected by explant ploidy level. In most cases, the regenerated shoots of autotetraploid (4x) genotypes were more robust than diploid (2x) ones. Regeneration efficiency of root explants declined from the apical to the basal root segment. Shoot and root biomass production of two month-old plants was not significantly different between 2x and 4x genotypes. Both 4x and 2x genotypes were characterized by the predominance of secoiridoid glucoside gentiopicrin in shoots and roots, which is followed by sweroside and swertiamarin. Loganic acid, loganin and secologanin were much less abundant. Methylbellidifolin was the major xanthone in both shoots and roots. Diploid plants showed higher biosynthetic capacity for the production of secoiridoids and xanthones in both shoots and roots. Results highlight a higher potential of diploid C. erythraea genotypes for biotechnology-based sustainable production of secoiridoids in comparison to tetraploid genotypes.

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Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Diploid vs. tetraploid Centaurium erythraea Rafn: A comparative study of regenerative in vitro potential and biosynthetic capacity

Filipović¹,

Šiler²,

Nestorović-Živković³

et al. 2019

Lekovite sirovine

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“…In citrus, polyploidy has been described to improve adaptation to different stresses and resilience ( Mouhaya et al, 2010 ; Podda et al, 2013 ; Allario et al, 2013 ; Tan et al, 2015 ; Tan et al, 2017 ; Dutra de Souza et al, 2017 ; Oliveira et al, 2017 ; Oustric et al, 2017 , 2018 ), and several teams worldwide have been developing rootstock breeding at the tetraploid level ( Grosser and Chandler, 2000 ; Grosser et al, 2000 , 2015 ; Ollitrault et al, 2000 , 2007 ; Guo et al, 2007 ; Grosser and Gmitter, 2010 ; Dambier et al, 2011 ; Guerra et al, 2016 ). Many of these programs focus on combinations of Citrus species with Poncirus , a related genus.…”

Section: Introductionmentioning

confidence: 99%

Preferential Homologous Chromosome Pairing in a Tetraploid Intergeneric Somatic Hybrid (Citrus reticulata + Poncirus trifoliata) Revealed by Molecular Marker Inheritance

et al. 2018

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The creation of intergeneric somatic hybrids between Citrus and Poncirus is an efficient approach for citrus rootstock breeding, offering the possibility of combining beneficial traits from both genera into novel rootstock lineages. These somatic hybrids are also used as parents for further tetraploid sexual breeding. In order to optimize these latter breeding schemes, it is essential to develop knowledge on the mode of inheritance in the intergeneric tetraploid hybrids. We assessed the meiotic behavior of an intergeneric tetraploid somatic hybrid resulting from symmetric protoplast fusion of diploid Citrus reticulata and diploid Poncirus trifoliata. The analysis was based on the segregation patterns of 16 SSR markers and 9 newly developed centromeric/pericentromeric SNP markers, representing all nine linkage groups of the Citrus genetic map. We found strong but incomplete preferential pairing between homologues of the same ancestral genome. The proportion of gametes that can be explained by random meiotic chromosome associations (τ) varied significantly between chromosomes, from 0.09 ± 0.02 to 0.47 ± 0.09, respectively, in chromosome 2 and 1. This intermediate inheritance between strict disomy and tetrasomy, with global preferential disomic tendency, resulted in a high level of intergeneric heterozygosity of the diploid gametes. Although limited, intergeneric recombinations occurred, whose observed rates, ranging from 0.09 to 0.29, respectively, in chromosome 2 and 1, were significantly correlated with τ. Such inheritance is of particular interest for rootstock breeding because a large part of the multi-trait value selected at the teraploid parent level is transmitted to the progeny, while the potential for some intergeneric recombination offers opportunities for generating plants with novel allelic combinations that can be targeted by selection.

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“…The hybrid phenotype can differ from the addition of parental effects given that allopolyploidization triggers gene expression changes and modifies epigenetics altering the phenotype (Bassene et al, 2009 , 2010 ; Dambier et al, 2011 ; Xu et al, 2014 ). Some studies discuss to what extent these changes are caused by de novo interactions established between genomes coming from different species (Hegarty et al, 2009 ) or to the ploidy gain (Dambier et al, 2011 ; Tan et al, 2017 ). Allopolyploidization, generated either by sexual or somatic hybridization, involves the coexistence of parental genomes in a single nucleus.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization and Stress Tolerance Evaluation of New Allotetraploid Somatic Hybrids Between Carrizo Citrange and Citrus macrophylla W. rootstocks

et al. 2018

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Polyploidy is one of the main forces that drives the evolution of plants and provides great advantages for breeding. Somatic hybridization by protoplast fusion is used in citrus breeding programs. This method allows combining the whole parental genomes in a single genotype, adding complementary dominant characters, regardless of parental heterozygosity. It also contributes to surpass limitations imposed by reproductive biology and quickly generates progenies that combine the required traits. Two allotetraploid somatic hybrids recovered from the citrus rootstocks—Citrus macrophylla (CM) and Carrizo citrange (CC)—were characterized for morphology, genome composition using molecular markers (SNP, SSR, and InDel), and their tolerance to iron chlorosis, salinity, and Citrus tristeza virus (CTV). Both hybrids combine the whole parental genomes even though the loss of parental alleles was detected in most linkage groups. Mitochondrial genome was inherited from CM in both the hybrids, whereas recombination was observed for chloroplastic genome. Thus, somatic hybrids differ from each other in their genome composition, indicating that losses and rearrangements occurred during the fusion process. Both inherited the tolerance to stem pitting caused by CTV from CC, are tolerant to iron chlorosis such as CM, and have a higher tolerance to salinity than the sensitive CC. These hybrids have potential as improved rootstocks to grow citrus in areas with calcareous and saline soils where CTV is present, such as the Mediterranean region. The provided knowledge on the effects of somatic hybridization on the genome composition, anatomy, and physiology of citrus rootstocks will be key for breeding programs that aim to address current and future needs of the citrus industry.

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Metabolic adaptation following genome doubling in citrus doubled diploids revealed by non-targeted metabolomics

Cited by 33 publications

References 52 publications

Diploid vs. tetraploid Centaurium erythraea Rafn: A comparative study of regenerative in vitro potential and biosynthetic capacity

Diploid vs. tetraploid Centaurium erythraea Rafn: A comparative study of regenerative in vitro potential and biosynthetic capacity

Preferential Homologous Chromosome Pairing in a Tetraploid Intergeneric Somatic Hybrid (Citrus reticulata + Poncirus trifoliata) Revealed by Molecular Marker Inheritance

Molecular Characterization and Stress Tolerance Evaluation of New Allotetraploid Somatic Hybrids Between Carrizo Citrange and Citrus macrophylla W. rootstocks

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