Metabolic activity, the chemical composition of biomass and photosynthetic activity of<i>Chlorella vulgaris</i>under different light spectra in photobioreactors

Kula, Monika; Rys, Magdalena; Możdżeń, Katarzyna; Skoczowski, Andrzej

doi:10.1002/elsc.201200184

Cited by 42 publications

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“…, in terrestrial plants, several cinnamic acids (ferulic, sinapsis, and caffeic acid) are responsible for the so‐called F450, whereas the F530 is attributed to compounds such as berberine, quercetin, and riboflavin. Similar shifts in the fluorescence spectra, suggesting changes in the composition of phenolic compounds, were observed previously during the growth of Chlorella vulgaris culture .…”

Section: Resultssupporting

confidence: 85%

“…According to Lang et al [41], in terrestrial plants, several cinnamic acids (ferulic, sinapsis, and caffeic acid) are responsible for the so-called F450, whereas the F530 is attributed to compounds such as berberine, quercetin, and riboflavin. Similar shifts in the fluorescence spectra, suggesting changes in the composition of phenolic compounds, were observed previously during the growth of Chlorella vulgaris culture [34,42]. Fluorescence emission spectra can produce many relevant information regarding plant responses to changing growth conditions, for example, the intensity and/or spectrum of light, temperature, and nutrient deficiency.…”

Section: Phenol Content and Blue-green Fluorescence Emission Spectrasupporting

confidence: 70%

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Far‐red dependent changes in the chemical composition of Spirulina platensis

Kula

Rys

Saja

et al. 2016

Engineering in Life Sciences

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Research Article Far-red dependent changes in the chemical composition of Spirulina platensisThe influence of far-red light (FRL) was studied on the chemical composition of Spirulina platensis biomass. The following light compositions were used during the culture white light, blue-red LED light (BRL) and BRL supplemented with FRL (BRFRL). Chlorophyll and phenol contents were measured by spectrophotometric methods, whereas presence of carotenoids, lipids, and phycobiliproteins were estimated by Fourier-transform Raman spectrometry. Additionally, phenol content was investigated by fluorescence intensity of algae culture in the range of 430-650 nm. The content of chlorophyll and phenols in algae cells depended on the spectral composition of light and was the highest under BRL (16.7 ± 0.5 and 9.1 ± 0.6, respectively). It was shown that there is a positive linear correlation (R = 0.902 at p < 0.0000001) between the ratio of relative fluorescence intensity of S. platensis suspensions at 450 nm to the suspensions at 540 nm (F450/F540) and the content of phenolic compounds in the biomass. Changes in the F450/F540 ratio can explain approximately 80% changes of phenol contents in algae cells. Spirulina platensis Raman spectra demonstrated that the biomass of algae growing under white light and BRL had a significantly higher intensity of phycobiliprotein bands than the algae growing under BRFRL.

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Section: Resultssupporting

confidence: 85%

Section: Phenol Content and Blue-green Fluorescence Emission Spectrasupporting

confidence: 70%

Far‐red dependent changes in the chemical composition of Spirulina platensis

Kula

Rys

Saja

et al. 2016

Engineering in Life Sciences

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“…The results obtained in the present study were not compared to those obtained for WL, because it was demonstrated earlier that WL is definitely the best source of light for C. vulgaris cultures in photobioreactors [10].…”

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confidence: 88%

“…In previous work we have demonstrated that the addition of FR to LED matrix emitting blue-R light (BR) light had a positive effect on the metabolism of microalgae [10]. Due to the fact that in that study the BRFRL (blue + R + FR light) source-composed of two FR LEDs 720 and 740 nm (with a peak at about 730 nm)-was used, we decided to investigate whether it was necessary to apply "such a complicated source of BRFRL."…”

mentioning

confidence: 99%

Far‐red light (720 or 740 nm) improves growth and changes the chemical composition of Chlorella vulgaris

Kula

Rys

Skoczowski

2014

Engineering in Life Sciences

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Research ArticleFar-red light (720 or 740 nm) improves growth and changes the chemical composition of Chlorella vulgaris Far-red (FR) light is crucial for the efficiency of photosynthesis and photomorphogenetic activity. This study investigated whether FR light at different wavelengths affects the Chlorella vulgaris biomass growth rate and chemical composition. For this purpose, FR was added to the blue-red (R) light at the wavelength of 720 nm (BRFR 720 L [blue + R + FR 720 nm light]) or 740 nm (BRFR 740 L [blue + R + FR 740 nm light]). BRFR 740 L allowed both higher growth rate and increased amount of total biomass compared to BRFR 720 L (blue + R + FR 720 nm light). The chemical composition of C. vulgaris biomass, analyzed by FT-Raman spectroscopy on lyophilized cells, significantly correlated with the applied FR component. The differences in lipid, carotenoid, and chlorophyll contents were particularly evident and all were higher in BRFR 740 L. Fluorescence emissions spectra of C. vulgaris cells were measured in the range 420-800 nm in the cuvette equipped with a magnetic stirrer preventing sedimentation of the cells during measurement. In the blue-green range (420-650 nm) fluorescence emission spectra indicated that changes in the chemical composition of phenolic compounds in the algae depended on the wavelength of FR used in LED matrices. This work showed that the use of FR of a wavelength 740 nm in the bioreactor's light source significantly improves biomass production of C. vulgaris cultures. IntroductionThe light, among the many factors influencing the growth and metabolism of the algae, is one of the most important but also most highly variable parameter. It has been shown that the spectral composition of light that exerts a positive influence on the growth of a particular strain of algae is not necessarily beneficial for growth of another strain [1]. Hence the importance of the selection of spectral composition of light. Both higher and lower plants have developed many photoreceptors to estimate the intensity of the wavelength and the direcCorrespondence: Prof. Andrzej Skoczowski (amskoczowski@gmail .com), Institute of Biology, Pedagogical University of Cracow, ul. Podchorążych 2, PL 30-084 Cracow, Poland.Abbreviations: BR, blue + red light; Chl, chlorophyll; F450, blue fluorescence; F530, green fluorescence; F690, red chlorophyll fluorescence; F735, far-red chlorophyll fluorescence; F450/F530, ratio of the blue to green fluorescence; F450/F690, fluorescence ratio blue/red; F450/F735, fluorescence ratio blue/far-red; F690/F735, chlorophyll fluorescence ratio red/far-red; FR, far-red; R, red light; WL, white light tion of the light [2,3]. Red (R) and blue light plays an important role in the photosynthesis and photomorphogenesis, thus influencing the development and metabolism of autotrophic plants. The essential light requirement of the photosynthesis process is the PAR (photosynthetically active radiation) range, that is from 400 to 700 nm (the end of absorption spectrum of the chlorophyll [Chl]). H...

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“…Furthermore, the accumulation of other internal components of microalgae would be also affected by pesticides. Some algae have been widely used in environmental study, such as Chara coralline, Microcystis aeruginosa, Scenedesmus and Chlorella species (Li et al 2008;McGinn et al 2012;Kula et al 2014;Liu et al 2014;Abinandan et al 2015). As recognition element, microalgae also become an extremely important part of the biosensor in detecting pesticides (Orellana et al 2007;Ebenezer et al 2012;Perullini et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Identification of pesticide varieties and concentrations by detecting characteristics ofChlorella pyrenoidosa

et al. 2015

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Although the low concentration level of pesticides does not have significant impact on the overall water environment, water would become worse due to the pollution accumulation in long-term. The method disclosed in this study can be used as a good example for pesticide detection with the help of microalgae. Raman microspectroscopy technology is a convenient and rapid tool to detect the characteristics of microalgae and contribute for pesticide detection.

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Metabolic activity, the chemical composition of biomass and photosynthetic activity ofChlorella vulgarisunder different light spectra in photobioreactors

Cited by 42 publications

References 43 publications

Far‐red dependent changes in the chemical composition of Spirulina platensis

Far‐red dependent changes in the chemical composition of Spirulina platensis

Far‐red light (720 or 740 nm) improves growth and changes the chemical composition of Chlorella vulgaris

Identification of pesticide varieties and concentrations by detecting characteristics ofChlorella pyrenoidosa

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