Metabbolism of perfused ruminant muscle

Coward, B. Jane; Buttery, P. J.

doi:10.1017/s0021859600041836

Cited by 9 publications

(9 citation statements)

References 48 publications

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“…Table 4. Comparison of ratios of individual amino acids to tyrosine for sheep biceps femoris muscle obtained in present study with similar unpublished data of D. B. Lindsay (personal communication) and with data on the amino acid content of hemidiaphragm (Coward and Buttery, 1982) and actin plus myosin (Kominz et al 1954) Amino The values are similar for most amino acids across the different studies, even though the methods of analysis were different. The outstanding discrepancies are in the ratios of glutamate, lysine and arginine to tyrosine.…”

Section: Discussionsupporting

confidence: 85%

“…The outstanding discrepancies are in the ratios of glutamate, lysine and arginine to tyrosine. The values calculated from the data of Kominz et al (1954) for the lysine: tyrosine and arginine: tyrosine ratios are 124 and 284% higher, respectively, than the mean of the corresponding values in the present study and those of D. B. Lindsay (personal communication) and Coward and Buttery (1982). A lower glutamate: tyrosine ratio has been observed by D. B. Lindsay (personal communication) but the value found in the present study is identical to that of Kominz et al (1954).…”

Section: Discussionsupporting

confidence: 56%

“…Coward and Buttery (1982) have expressed their hemidiaphragm muscle protein values as ratios of individual amino acids to tyrosine. These ratios are used in Table 4 as bases for comparison between studies.…”

Section: Discussionmentioning

confidence: 99%

“…In these studies, and in most others on human muscle, the data of Kominz et al (1954) on muscle amino acid composition was used. Coward and Buttery (1982) measured the amino acid content of sheep hemidiaphragm, but data on individual skeletal muscles of sheep are largely unavailable.…”

Section: Introductionmentioning

confidence: 99%

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Development of a Sheep Hind-Limb Muscle Preparation for Metabolic Studies

Teleni¹,

Annison²

1986

Aust. Jnl. Of Bio. Sci.

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A sheep hind-limb preparation used for the study of muscle metabolism by arteriovenous (A V) difference procedures was validated by identifying the muscles which contribute to venous drainage at different positions along the lateral saphenous vein. Dissection of the hind limbs of six mature sheep (three wethers and three ewes) showed that venous blood from the plantar group (M. gastrocnemius, M. soleus, M. plantaris, M. flexo digitorum profundus), and from M. semitendinosus, M. biceps femoris, M. gracilis, M. pectineus and M. adductor muscles entered the lateral saphenous vein but the position of the tip of the blood sampling catheter was found to be critical. In order to sample venous blood from all of the muscles listed above, and to minimize the contribution of blood from non-muscular tissues, blood samples must be taken 25-26 cm from the junction of the cranial and caudal branches of the lateral saphenous vein (for average size sheep of body length about 108 cm and height at withers about 73 cm).The estimation of sheep hind-limb muscle mass is a necessary concomitant of A V difference studies, and a combined tritiated water and dye-dilution procedure has been used to measure both muscle mass and blood flow.The muscle mass estimated in vivo by this technique was closely similar to the true muscle mass obtained by dissection, the range of values of the difference between true and calculated muscle mass expressed as percentage of the true mass being 0·5-16%. It is concluded that these techniques are sufficiently accurate for use in the quantitation of exchange of metabolites across the hind-limb muscle preparation.Patterns of amino acid uptake and release by muscle need to be related to the amino acid profile of the tissue, and the amino acid content of a representative muscle, M. biceps femoris, was determined, and the results compared with published data.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionsupporting

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development of a Sheep Hind-Limb Muscle Preparation for Metabolic Studies

Teleni¹,

Annison²

1986

Aust. Jnl. Of Bio. Sci.

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“…Besides cloning in laboratory rodents (rats, mice, guinea-pigs) and humans, partial or complete sequences of GLUT1 and than 10-fold in rats [16] but less than 2-fold in sheep [24].…”

Section: Cloning Of Glut Cdnas In Livestock Speciesmentioning

confidence: 99%

Facilitative glucose transporters in livestock species

Hocquette

Abé

2000

Reprod. Nutr. Dev.

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-The study of facilitative glucose transporters (GLUT) requires carefully done immunological experiments and sensitive molecular biology approaches to identify the various mechanisms which control GLUT expression at the RNA and protein levels. The cloning of species-specific GLUT cDNAs showed that GLUT4 and GLUT1 diverge less among species than other GLUT isoforms. The key role of GLUT in glucose homeostasis has been demonstrated in livestock species. In vitro studies have suggested specific roles of GLUT1 and GLUT3 in avian cells. In vivo studies have demonstrated a regulation of GLUTs (especially of GLUT4) by nutritional and hormonal factors in pigs and cattle, in lactating cows and goats and throughout the foetal life in the placenta and tissues of lambs and calves. All these results suggest that any changes in GLUT expression and activity (such as GLUT4 in muscles) could modify nutrient partitioning and tissue metabolism, and hence, the qualities of animal products (milk, meat).glucose transporter / pig / ruminant / poultry Résumé -Les transporteurs du glucose à diffusion facilitée chez les animaux de ferme. L'étude des transporteurs du glucose à diffusion facilitée (GLUT) nécessite des techniques en immunologie ou en biologie moléculaire précises et sensibles pour étudier la régulation de leur expression au niveau ARNm ou protéique. Le clonage d'ADNc des différents GLUT a montré que GLUT4 et GLUT1 divergent moins entre espèces que les autres isoformes de GLUT. Le rôle important des GLUT dans le contrôle de l'homéostasie du glucose a été démontré chez les animaux de ferme. Des études in vitro ont suggéré un rôle spécifique de GLUT1 et de GLUT3 dans les cellules aviaires. Des études in vivo ont mis en évidence une régulation des GLUT (notamment GLUT4) par des facteurs nutritionnels ou hormonaux chez le porc et le bovin, chez la vache ou la chèvre en lactation, et tout au long de la vie foetale dans le placenta et les tissus de l'agneau ou du veau. L'ensemble de ces résultats suggère que tout changement dans l'expression et l'activité des GLUT (tel que de GLUT4 dans les muscles) peut modifier la répartition des nutriments et le métabolisme tissulaire, et affecter ainsi la qualité des produits animaux (lait, viande).transporteur du glucose / porc / ruminant / volaille Reprod. Nutr. Dev. 40 (2000) 517-533 517

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Catabolism and synthesis of amino acids in skeletal muscle: their significance in monogastric mammals and ruminants

Teleni¹

1993

Aust. J. Agric. Res.

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The enhanced rate of synthesis and catabolism of amino acids in, and their release from, skeletal muscle, particularly during fasting, exercise and metabolic acidosis, highlight the integrative role of muscle in protein-energy metabolism. This review discusses aspects of such changes in muscles of monogastric mammals and ruminants. The glucose-alanine cycle, as it was originally proposed, has been well substantiated by studies using human and rat muscles. An alternative proposal, which suggested that other amino acids make a major contribution to the carbon skeleton of alanine synthesized in muscle, is less convincing, since some of the inhibitors and substrates used in relevant studies have been demonstrated to have multiple rather than specific effects. In the ruminant, the glucose-alanine cycle is quantitatively less significant than in human. The probable reason for this difference is the limited available pyruvate in ruminant muscle for transamination to alanine. This may be due to a lower carbon flux through the glycolytic pathway and/or to significant activity of the anaplerotic enzyme, pyruvate carboxylase. It is suggested that glutamine is the more important carrier of carbon and nitrogen out of skeletal muscle and that alanine may serve only as an ancillary vehicle to transport carbon and nitrogen when the availability of pyruvate for transamination in muscle is high. The more diverse role of glutamine (cf. alanine) in acid-base balance, as a respiratory fuel in the cells of the immune system and the epithelia of the small intestine, where it may also be converted to alanine for subsequent gluconeogenesis in the liver, is consistent with this suggestion.

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Metabbolism of perfused ruminant muscle

Cited by 9 publications

References 48 publications

Development of a Sheep Hind-Limb Muscle Preparation for Metabolic Studies

Development of a Sheep Hind-Limb Muscle Preparation for Metabolic Studies

Facilitative glucose transporters in livestock species

Catabolism and synthesis of amino acids in skeletal muscle: their significance in monogastric mammals and ruminants

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