Metabarcoding fecal samples to investigate spatiotemporal variation in the diet of the endangered Westland Petrel ( Procellaria westlandica )

Abstract

As top predators, seabirds can be indirectly impacted by climate variability and commercial fishing activities through changes in marine communities. However, high mobility and foraging behavior enable seabirds to exploit prey distributed patchily in time and space. Despite this environmental adaptability, seabirds are the world's most threatened birds and there is, therefore, an urge to acquire information about their ecological and foraging requirements through the study of their diet. Traditionally, the diet of seabirds is assessed through the morphological identification of prey remains in regurgitates. This sampling method is invasive for the bird and limited in terms of taxonomic resolution. However, the recent progress in DNA-based approaches is now providing a noninvasive means to more comprehensively and accurately characterize animal diets. We used a noninvasive metabarcoding approach to characterize the diet of the Westland Petrel (Procellaria westlandica), which is an endangered burrowing species, endemic to the South Island of New Zealand. We collected 99 fresh fecal samples in 2 different seasons and in 2 different subcolonies. Our aim was to describe the Westland Petrel's diet and to investigate seasonal and spatial variations to their diet to understand the feeding requirements of this species. We also specifically investigated potential links with the New Zealand fishery industry to inform efficient conservation practices. Our noninvasive dietary DNA (dDNA) approach also highlighted significant differences in diet between seasons (incubation vs. chick-rearing season) and between sampling sites (two subcolonies 1.5 km apart). This suggests plasticity in the foraging strategy of the Westland Petrel. We found that amphipods were the most common prey, followed by cephalopods, and fish. Our findings could be the result of natural foraging behavior but also suggest a close link between the composition of prey items and New Zealand's commercial fishing activities. In particular, the high abundance of amphipods could be the result of Westland Petrels feeding on discarded fisheries waste (fish guts). This close relationship to New Zealand's fisheries may put stress on the resilience of the Westland Petrel. This valuable knowledge about Westland Petrel foraging needs is key to design efficient conservation plans for this iconic species. We illustrate how dDNA can inform the conservation of endangered or at-risk species that have elusive foraging behaviors. Métacodage à barres d'échantillons fécaux pour étudier les variations spatio-temporelles du régime alimentaire du Puffin du Westland (Procellaria westlandica), espèce en voie de disparitionRÉSUMÉ. En tant que prédateurs supérieurs, les oiseaux marins peuvent être indirectement touchés par la variabilité du climat et les activités de pêche commerciale par l'intermédiaire de changements sur le plan des communautés marines. Cependant, leur grande mobilité et leur comportement de recherche de nourriture leur permettent d'exploiter des proies réparties dans l...

Overview of Predation by Birds, Cephalopods, Fish and Marine Mammals on Marine Benthic Amphipods

“…Similarly, ref. [35] collected fresh fecal samples to identify the prey of the Westland petrel Procellaria westlandica Falla, 1946, a species endemic to the South Island of New Zealand. Ref.…”

“…In this way, ref. [35] used a non-invasive metabarcoding approach to characterize the diet of the Westland petrel (Procellaria westlandica), an endangered burrowing species endemic to the South Island of New Zealand. Similarly, ref.…”

“…Using a non-invasive DNA metabarcoding approach, ref. [35] recognized the presence of talitroidean amphipods in the diet of the Westland petrel Procellaria westlandica from the South Island of New Zealand.…”