Meta-Omics reveal the metabolic acclimation of freshwater anammox bacteria for saline wastewater treatment

Ji, Xiaoming; Wang, Yong-Li; Zhan, Xinmin; Wu, Zhuoying; Lee, Po‐Heng

doi:10.1016/j.jclepro.2022.132184

Cited by 9 publications

(2 citation statements)

References 62 publications

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“…Most genomes contained transporters belonging to the Nitrate/Nitrite Porter (NNP) family, including narK, nrtP, and nasA for nitrate and nitrite membrane transport (Moir and Wood, 2001). Consistent with previously reported anammox metagenomes (Ji et al, 2022;Wang et al, 2022), AMX01 contained Nitrate Transporter superfamily (NRT) nitrate/nitrite transporters, the high affinity nitrate/nitrite transporter nrtB, and a polytopic membrane transporter specifically for nitrite, nirC. Several genomes, excluding those of anammox bacteria, also encoded genes for putative formate-nitrite transporters from the formatenitrate transporter (FNT) superfamily, including formate channel focA and formate permease fdhC.…”

Section: Nitrogen Cycle Gene Abundancessupporting

confidence: 87%

Synergistic interactions between anammox and dissimilatory nitrate reducing bacteria sustains reactor performance across variable nitrogen loading ratios

et al. 2023

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Anaerobic ammonium oxidizing (anammox) bacteria are utilized for high efficiency nitrogen removal from nitrogen-laden sidestreams in wastewater treatment plants. The anammox bacteria form a variety of competitive and mutualistic interactions with heterotrophic bacteria that often employ denitrification or dissimilatory nitrate reduction to ammonium (DNRA) for energy generation. These interactions can be heavily influenced by the influent ratio of ammonium to nitrite, NH4+:NO2−, where deviations from the widely acknowledged stoichiometric ratio (1:1.32) have been demonstrated to have deleterious effects on anammox efficiency. Thus, it is important to understand how variable NH4+:NO2− ratios impact the microbial ecology of anammox reactors. We observed the response of the microbial community in a lab scale anammox membrane bioreactor (MBR) to changes in the influent NH4+:NO2− ratio using both 16S rRNA gene and shotgun metagenomic sequencing. Ammonium removal efficiency decreased from 99.77 ± 0.04% when the ratio was 1:1.32 (prior to day 89) to 90.85 ± 0.29% when the ratio was decreased to 1:1.1 (day 89–202) and 90.14 ± 0.09% when the ratio was changed to 1:1.13 (day 169–200). Over this same timespan, the overall nitrogen removal efficiency (NRE) remained relatively unchanged (85.26 ± 0.01% from day 0–89, compared to 85.49 ± 0.01% from day 89–169, and 83.04 ± 0.01% from day 169–200). When the ratio was slightly increased to 1:1.17–1:1.2 (day 202–253), the ammonium removal efficiency increased to 97.28 ± 0.45% and the NRE increased to 88.21 ± 0.01%. Analysis of 16 S rRNA gene sequences demonstrated increased relative abundance of taxa belonging to Bacteroidetes, Chloroflexi, and Ignavibacteriae over the course of the experiment. The relative abundance of Planctomycetes, the phylum to which anammox bacteria belong, decreased from 77.19% at the beginning of the experiment to 12.24% by the end of the experiment. Analysis of metagenome assembled genomes (MAGs) indicated increased abundance of bacteria with nrfAH genes used for DNRA after the introduction of lower influent NH4+:NO2− ratios. The high relative abundance of DNRA bacteria coinciding with sustained bioreactor performance indicates a mutualistic relationship between the anammox and DNRA bacteria. Understanding these interactions could support more robust bioreactor operation at variable nitrogen loading ratios.

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Section: Nitrogen Cycle Gene Abundancessupporting

confidence: 87%

Synergistic interactions between anammox and dissimilatory nitrate reducing bacteria sustains reactor performance across variable nitrogen loading ratios

et al. 2023

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“…Generally, the physiological metabolism of anammox bacteria is initially inhibited under salinity conditions which deteriorates the nitrogen removal performance, and then the anammox bacteria gradually adapted to the salinity to achieve efficient nitrogen removal. − Previous studies have extensively investigated the two main salinity adaptation strategies of anammox bacteria to maintain efficient nitrogen removal in the anammox process during saline wastewater treatment, including the salt ingestion and compatible solutes. , The salt ingestion strategy is to pump inorganic ions into the cell membrane to help microbes to balance osmotic pressure . The compatible solutes are absorbed or synthesized to maintain the cell turgor pressure by anammox bacteria .…”

Section: Introductionmentioning

confidence: 99%

Potential Role of the Anammoxosome in the Adaptation of Anammox Bacteria to Salinity Stress

Xiong,

Liao,

Guo

et al. 2024

Environ. Sci. Technol.

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The underlying adaptative mechanisms of anammox bacteria to salt stress are still unclear. The potential role of the anammoxosome in modulating material and energy metabolism in response to salinity stress was investigated in this study. The results showed that anammox bacteria increased membrane fluidity and decreased mechanical properties by shortening the ladderane fatty acid chain length of anammoxosome in response to salinity shock, which led to the breakdown of the proton motive force driving ATP synthesis and retarded energy metabolism activity. Afterward, the fatty acid chain length and membrane properties were recovered to enhance the energy metabolic activity. The relative transmission electron microscopy (TEM) area proportion of anammoxosome decreased from 55.9 to 38.9% under salinity stress. The 3D imaging of the anammox bacteria based on Synchrotron soft X-ray tomography showed that the reduction in the relative volume proportion of the anammoxosome and the concave surfaces was induced by salinity stress, which led to the lower energy expenditure of the material transportation and provided more binding sites for enzymes. Therefore, anammox bacteria can modulate nitrogen and energy metabolism by changing the membrane properties and morphology of the anammoxosome in response to salinity stress. This study broadens the response mechanism of anammox bacteria to salinity stress.

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Biochar‐Assisted Anaerobic Ammonium Oxidation

Tian,

Tang,

et al. 2023

Biochar Applications for Wastewater Treatment

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Meta-Omics reveal the metabolic acclimation of freshwater anammox bacteria for saline wastewater treatment

Cited by 9 publications

References 62 publications

Synergistic interactions between anammox and dissimilatory nitrate reducing bacteria sustains reactor performance across variable nitrogen loading ratios

Synergistic interactions between anammox and dissimilatory nitrate reducing bacteria sustains reactor performance across variable nitrogen loading ratios

Potential Role of the Anammoxosome in the Adaptation of Anammox Bacteria to Salinity Stress

Biochar‐Assisted Anaerobic Ammonium Oxidation

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