Meta-analysis of toxicity and teratogenicity of 133 chemicals from zebrafish developmental toxicity studies

Comparison of toxicity values across zebrafish early life stages and mammalian studies: Implications for chemical testing

“…We utilized the dataset of 120 publications from Ducharme et al (2013) [11] and added 77 additional published studies from the literature available via PubMed through June 1, 2013 (listed in Supplemental Table 1). Inclusion criteria were: Publications describing exposure to environmental or industrial chemicals in zebrafish; Treatment and scoring period from 0 to 7 days post fertilization (dpf); Presentation of a statistically significant effect after exposure to the test chemical that was different from unexposed or vehicle exposed fish.…”

From cutting edge to guideline: A first step in harmonization of the zebrafish embryotoxicity test (ZET) by describing the most optimal test conditions and morphology scoring system

“…It can be argued that some compounds would need specific embryonic stages to elicit their teratogenic effects, especially if specific receptors/targets need to be present to elude the teratogenic effect. Nevertheless, it appears that most teratogenic compounds can elicit teratogenic effects at very early life stages, as somite formation and heart development seem to be by far the most sensitive structures in the developing ZF to teratogenic compounds [35]. In the ZF, organogenesis of the main organs is completed by 72 hpf, most organs are fully developed by 96 hpf, and by 120 hpf the development of the larva is completed [18,36].…”

“…Prominence was given to skeletal deformities and heart and yolk edema, as meta-analysis studies have found these endpoints to be the most common toxic targets of teratogenic compounds and good predictors of teratogenicity [35].…”

Test driving ToxCast: endocrine profiling for 1858 chemicals included in phase II

“…A graphical user interface (GUI) is available for ToxPi that provides a user-friendly way to (re)combine data from ToxCast and/or any other source [5]. This framework has been used for meta-analysis of studies across labs, exploration of cardiotoxicity endpoints from induced pluripotent stem cells, and integration of chemistry-based structure-activity relationship models (SAR) with biological assay results [14–16]. The utility of such a large chemical screening effort—where each chemical has full-rank assay data—will be realized when researchers examine focused screening results within the broad context of ToxCast and related screening and testing efforts.…”