Meta-Analysis of miR-146a Polymorphisms Association with Coronary Artery Diseases and Ischemic Stroke

Abstract

Coronary artery disease (CAD) and ischemic stroke (IS) are manifestations of atherosclerosis, with a high death rate. miR-146a is a microRNA that participates in the progress of CAD and IS. A single nucleotide polymorphism (SNP) in the precursor of miR-146a, rs2910164, was found to be associated with the risks of CAD and IS. However, the results were inconsistent and inconclusive. A meta-analysis was performed to assess the relationship of rs2910164 and CAD as well as IS susceptibility. The database Pubmed, Embase, Cochrane Central Register of Controlled Trials (CENTRAL), Chinese National Knowledge Infrastructure (CNKI), and Chinese Biomedical Literature Database (CBM) were searched for related studies. Crude odds ratios with 95% confidence intervals were used to investigate the strength of the association by random- or fixed-effect model. A total of eight studies, with 3138 cases and 3097 controls were identified for the meta-analysis. The results shows that rs2910164 is associated with the risk of CAD significantly in allelic model (OR = 0.86), homozygous model (OR = 0.70), heterozygous model (OR = 0.80) and dominant model (OR = 0.76). The subjects carrying the GG genotype, GG + GC genotype or G allele are at lower risks of CAD. For the susceptibility of IS, there are no significant associations between rs2910164 and total studies. However, in subgroup analysis by sample size and ethnicity, the GG, GG + GC and G allele of rs2910164 are found to be associated with higher risks of IS in large sample size group and in Koreans, under homozygous and dominant models. In conclusion, the current meta-analysis suggests lower risks of CAD for GG, GG + GC genotype and G allele of rs2910164, while rs2910164 is not associated with the risk of IS. Thus rs2910164 might be recommended as a predictor for susceptibility of CAD, but not IS.

MicroRNA-331 and microRNA-151-3p as biomarkers in patients with ST-segment elevation myocardial infarction

“…Firstly, we selected three study miRs that were significantly deregulated in the initial screening (miR-331, miR-151-3p and miR-518d) and have not yet been associated with cardiovascular disease. We further selected miR-146a, miR-145, miR-155, miR-24 and miR-323p, which were previously linked to ACS, as positive controls [44][45][46][47][48][49] . MicroRNA-208 and miR-499 associated with myocardial necrosis were selected as controls of timely sampling, since our aim was to detect miRs associated with plaque rupture rather than myocardial necrosis 35 .…”

Insufficient sleep is associated with a pro‐atherogenic circulating microRNA signature

“…The key finding of the present study is that habitual insufficient sleep (<7 h night −1 ) is associated with disruption in circulating levels of miR‐125a, miR‐126 and miR‐146a. Altered circulating profiles of these vascular‐related miRNAs have been linked to vascular dysfunction and increased CVD risk and events (Bao et al., ; Hao et al., ; van Empel et al., ; Zampetaki et al., ). To our knowledge, this the first study to determine the influence of short sleep duration on circulating miRNA signatures.…”

“…Thus, it is plausible that lower miR‐146a levels might contribute to the increased inflammatory burden associated with habitual short nightly sleep (Aronica et al., ). Moreover, considering that lower circulating miR‐146a levels have been shown to be predictive of atherosclerosis and coronary events (Bao et al., ; Ramkaran, Khan, Phulukdaree, Moodley, & Chuturgoon, ), the negative influence of insufficient sleep on miR‐146a may have yet untold cardiovascular consequences.…”

Research of expression quantity of serum miR-146a and miR-146b in patients with acute cerebral infarction before and after the intervention of rosuvastatin

“…miR146a and miR-146b are two kinds of miRNA closely correlated with cardiovascular and cerebrovascular diseases. There was a research that has proved that miR-146a and miR-146b tend to show abnormal low expression in the body of patients with various cardiacerebrovascular diseases and can affect the process of inflammatory response [8,9]. The abnormal low expression of miR-146a and miR146b in the body of patients with acute cerebral infarction will lead to a decrease of inhibition of TLR2 and TLR4 by miRNAs and further up-regulate the expression of TLR2 and TLR4 and increase the secretion of downstream inflammatory factors, by which we can conclude that rosuvastatin may affect the secretion of inflammatory factors mediated by TLR2 and TLR4 through regulating the expression of miR-146a and miR-146b, which further plays an anti-inflammatory role in the treatment process of acute cerebral infarction.…”