Meta-Analysis of Genetic Studies from Journals Published in China of Ischemic Stroke in the Han Chinese Population

Abstract

Background: The aim of this study was to confirm the nature and number of genes contributing to stroke risk and qualify the genetic risk of each susceptibility gene in the Han Chinese population. Methods: After collecting all case-control studies related to DNA polymorphism of any candidate gene for ischemic stroke in Han Chinese, strict selection criteria and exclusion criteria were determined and different effect models were used according to the difference in heterogeneity. Meta-analyses were carried out by Revman 4.0 software and the publication bias was further evaluated through calculation of fail-safe numbers in the included gene polymorphisms. Results: Seventy-six studies were included in the meta-analyses which were all published in mainland China and referred to 6 candidate genes and 7 polymorphisms. Among the gene polymorphisms tested in the study, association of gene polymorphisms with increasing risk of ischemic stroke was confirmed in 6 polymorphisms including angiotensin-converting enzyme insertion/deletion (ACE I/D; OR = 1.87, 95% CI = 1.45–2.42), methylenetetrahydrofolate reductase (MTHFR) C677T (OR = 1.55, 95% CI = 1.26–1.90), plasminogen activator inhibitor 1 (PAI-1) 4G/5G (OR = 1.79, 95% CI = 1.20–2.67), β-fibrinogen (β-Fg) –455A/G (OR = 1.48, 95% CI = 1.14–1.92), β-Fg –148T/C (OR = 1.72, 95% CI = 1.42–2.07), apolipoprotein E (ApoE) Ε2–4 (OR = 2.39, 95% CI = 1.94–2.95). Because of the obvious publication bias, the association between paraoxonase 1 (PON-1) polymorphisms and stroke risk was not established although the OR of the meta-analysis suggested a positive result (OR = 1.14, 95% CI = 1.01–1.35). Conclusions: ACE D/I, MTHFR C677T, β-Fg –455A/G, β-Fg –148T/C, PAI-1 4G/5G, and ApoE Ε2–4 were associated with risk of ischemic stroke in Han Chinese.

Genetics of stroke

“…Homozygous presence of D alleles is associated with higher plasma ACE activity. Case-control studies [65,66] revealed that ACE D/D genotypes contribute to ischemic stroke incidence, and this may be due to the effect on essential hypertension. In one study [67] , the association between the ACE D allele and lacunar infarction was positive in the normotensive patients, but the association diminished in hypertensive patients, suggesting an independent effect of ACE D alleles on the lacunar infarction.…”

Effects of interferon alpha treatment on recurrence and survival after complete resection or ablation of hepatocellular carcinoma: A meta‐analysis of randomized controlled trials

“…We evaluated publication bias by calculating the fail-safe number (Nfs) of meta-analysis. 39 If the Nfs was smaller than the number of observed studies, the meta-analysis result might have a significant publication bias. The statistical significance was set at p 5 0.05 [Nfs0.05 5 ( P Z/1.64) 2 2 k; k means the number of studies included in meta-analysis].…”

Correlation between PPARg2 gene Pro12Ala polymorphism and cerebral infarction in an Inner Mongolian Han Chinese population

“…Hypertension, diabetes mellitus, and dyslipidemia have been demonstrated by others to be related to cerebral infarction, and are recognized as traditional risk factors (Park et al, 2014). Genetics and molecular biology studies have revealed the existence of genetic predisposition to cerebral infarction, and have identified various polymorphisms associated with this condition (Domingues-Montanari et al, 2008;Xu et al, 2008). Atherosclerosis, the inflammatory response, and impaired vascular endothelial function play an important role in the development of cerebral infarction (Pucci et al, 2011;Soskić et al, 2011).…”