Meta-analysis of Chicken – Salmonella infection experiments

Pas, M.F.W. te; Hulsegge, Ina; Schokker, Dirkjan; Smits, M.A.; Fife, Mark; Zoorob, Rima; Endale, Marie-Laure; Rebel, Johanna M.J.

doi:10.1186/1471-2164-13-146

Cited by 14 publications

(12 citation statements)

References 46 publications

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“…However, the number of differentially expressed genes in the MDR S . Heidelberg-challenged turkeys seemed minimal compared to other gene expression studies of poultry in response to Salmonella ( 28 ). Moreover, a recent study by our group profiling the transcriptome of 3-week-old commercial turkeys in response to S .…”

Section: Resultsmentioning

confidence: 89%

Characterization of a Multidrug-Resistant Salmonella enterica Serovar Heidelberg Outbreak Strain in Commercial Turkeys: Colonization, Transmission, and Host Transcriptional Response

Bearson

Looft

et al. 2017

Front. Vet. Sci.

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In recent years, multidrug-resistant (MDR) Salmonella enterica serovar Heidelberg (S. Heidelberg) has been associated with numerous human foodborne illness outbreaks due to consumption of poultry. For example, in 2011, an MDR S. Heidelberg outbreak associated with ground turkey sickened 136 individuals and resulted in 1 death. In response to this outbreak, 36 million pounds of ground turkey were recalled, one of the largest meat recalls in U.S. history. To investigate colonization of turkeys with an MDR S. Heidelberg strain isolated from the ground turkey outbreak, two turkey trials were performed. In experiment 1, 3-week-old turkeys were inoculated with 108 or 1010 CFU of the MDR S. Heidelberg isolate, and fecal shedding and tissue colonization were detected following colonization for up to 14 days. Turkey gene expression in response to S. Heidelberg exposure revealed 18 genes that were differentially expressed at 2 days following inoculation compared to pre-inoculation. In a second trial, 1-day-old poults were inoculated with 104 CFU of MDR S. Heidelberg to monitor transmission of Salmonella from inoculated poults (index group) to naive penmates (sentinel group). The transmission of MDR S. Heidelberg from index to sentinel poults was efficient with cecum colonization increasing 2 Log10 CFU above the inoculum dose at 9 days post-inoculation. This differed from the 3-week-old poults inoculated with 1010 CFU of MDR S. Heidelberg in experiment 1 as Salmonella fecal shedding and tissue colonization decreased over the 14-day period compared to the inoculum dose. These data suggest that young poults are susceptible to colonization by MDR S. Heidelberg, and interventions must target turkeys when they are most vulnerable to prevent Salmonella colonization and transmission in the flock. Together, the data support the growing body of literature indicating that Salmonella establishes a commensal-like condition in livestock and poultry, contributing to the asymptomatic carrier status of the human foodborne pathogen in our animal food supply.

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Section: Resultsmentioning

confidence: 89%

Characterization of a Multidrug-Resistant Salmonella enterica Serovar Heidelberg Outbreak Strain in Commercial Turkeys: Colonization, Transmission, and Host Transcriptional Response

Bearson

Looft

et al. 2017

Front. Vet. Sci.

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“…; Te Pas et al . ). However, chicken exhibit a different immune response and eliminating mechanism for S .…”

Section: Introductionmentioning

confidence: 97%

Identification of the crucial genes in the elimination and survival process of Salmonella enterica ser. Pullorum in the chicken spleen

Wang

et al. 2017

Animal Genetics

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Salmonella enterica ser. Pullorum is one of the most easily re-infecting pathogens in poultry production because of its mechanism of escaping from immune elimination. We used the transcriptome method to investigate the variation in gene expression in chicken spleen resulting from the interaction between hosts and S. Pullorum in the survival process. The expression of various genes related to the maturation and activation of B cells was activated before S. Pullorum was eliminated, which might help S. Pullorum escape from the elimination process. The suppression of some genes involved in the fusion of autophagosomes and lysosomes, such as MYO6, was identified and may be regulated by the secretion systems of S. Pullorum. In addition, a large proportion of these differentially expressed genes could be localized in the identified quantitative trait loci regions associated with the antibody response to bacteria. Collectively, these identified genes provided an outline for further understanding the interaction between chicken immune cells and S. Pullorum in chicken spleen.

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“…Meta-analyses combine the information gathered from multiple studies addressing similar questions, but often the data H Corresponding author. E-mail: sjlamont@iastate.edu from such studies originate from different individuals or use mRNA that was collected under different experimental conditions (3,5,7,25). In the current study, transcriptome patterns in spleen and peripheral blood leukocytes (PBLs) from the same individual chickens were compared in a bianalysis to better understand the host response to APEC.…”

mentioning

confidence: 99%

Strong Concordance Between Transcriptomic Patterns of Spleen and Peripheral Blood Leukocytes in Response to Avian Pathogenic Escherichia coli Infection

Sandford¹,

Orr²,

Li³

et al. 2012

Avian Diseases

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Avian pathogenic Escherichia coli (APEC) causes morbidity in chickens and exhibits zoonotic potential. Understanding host transcriptional responses to infection aids the understanding of protective mechanisms and serves to inform future colibacillosis control strategies. Transcriptomes of spleen and peripheral blood leukocytes (PBLs) of the same individual birds in response to APEC infection were compared to identify common response patterns and connecting pathways. More than 100 genes in three contrasts examining pathology and infection status were significantly differentially expressed in both tissues and similarly regulated. Tissue-specific differences in catalytic activity, however, appear between birds with mild and severe pathology responses. Early expression differences, between birds with severe pathology and uninfected controls, in the mitogen-activated protein kinase pathway in PBLs precede spleen responses in the p53 and cytokine-cytokine receptor pathways. Tissue bianalysis is useful in identifying genes and pathways important to the response to APEC, whose role might otherwise be underestimated in importance. Understanding host transcriptional responses to infection aids the understanding of protective mechanisms and serves to inform future colibacillosis control strategies. Transcriptomes of spleen and peripheral blood leukocytes (PBLs) of the same individual birds in response to APEC infection were compared to identify common response patterns and connecting pathways. More than 100 genes in three contrasts examining pathology and infection status were significantly differentially expressed in both tissues and similarly regulated. Tissue-specific differences in catalytic activity, however, appear between birds with mild and severe pathology responses. Early expression differences, between birds with severe pathology and uninfected controls, in the mitogen-activated protein kinase pathway in PBLs precede spleen responses in the p53 and cytokine-cytokine receptor pathways. Tissue bianalysis is useful in identifying genes and pathways important to the response to APEC, whose role might otherwise be underestimated in importance.RESUMEN. Marcada concordancia entre los patrones transcriptómicos de bazo y leucocitos de sangre periférica durante la respuesta a la infección por Escherichia coli patógena aviar.La Escherichia coli patogénica para las aves (APEC) causa morbilidad en pollos y representa un potencial zoonótico. El conocimiento de las respuestas transcripcionales del huésped durante la infección contribuye a la comprensión de los mecanismos de protección y sirve para instrumentar futuras estrategias para el control de la colibacilosis. Se compararon los transcriptomas de bazo y de leucocitos de sangre periférica (PBL) de las mismas aves individuales durante la respuesta a la infección por E. coli patogénica para las aves para identificar los patrones de respuesta comunes y los mecanismos de conexión. Más de 100 genes en tres contrastes que examinaron la patología y el estado de infección ...

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Meta-analysis of Chicken – Salmonella infection experiments

Cited by 14 publications

References 46 publications

Characterization of a Multidrug-Resistant Salmonella enterica Serovar Heidelberg Outbreak Strain in Commercial Turkeys: Colonization, Transmission, and Host Transcriptional Response

Characterization of a Multidrug-Resistant Salmonella enterica Serovar Heidelberg Outbreak Strain in Commercial Turkeys: Colonization, Transmission, and Host Transcriptional Response

Identification of the crucial genes in the elimination and survival process of Salmonella enterica ser. Pullorum in the chicken spleen

Strong Concordance Between Transcriptomic Patterns of Spleen and Peripheral Blood Leukocytes in Response to Avian Pathogenic Escherichia coli Infection

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