Meta-analysis data of the accuracy of tests for meat adulteration by real-time PCR

Iskakova, Aisha; Abitayeva, Gulyaim; Beisenbayevich, Abeev Arman; Serikovna, Sarmurzina Zinigul

doi:10.1016/j.dib.2022.107972

Cited by 8 publications

(2 citation statements)

References 14 publications

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“…One of the main predictive indicators that allow classifying PCR as the most accurate diagnostic method is the likelihood ratio of a positive result ( Iskakova et al ., 2022 ). Statistical calculations of quantitative indicators obtained during the research in the framework of this study indicate that the probability of an accurate diagnosis in PCR is several times higher than when using conventional approaches in the diagnosis of skin mycoses.…”

Section: Discussionmentioning

confidence: 99%

Comparative assessment of regulated methods and PCR in the diagnosis of trichophytosis in veterinary mycology

Umitzhanov,

Abdiramanova,

Abutalip

et al. 2023

Open Vet J

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Background: There is an increase in the incidence of human and animal infectious skin diseases of fungal aetiology in the world. The main source of infecting the population has become agricultural and stray animals. Aim: The objective of this study was to examine the morphophysiological and microbiological characteristics of pathogenic fungi belonging to the species Trichophyton verrucosum. This species is known to cause diseases in both humans and livestock in Kazakhstan. Additionally, the study aimed to assess the feasibility of using the polymerase chain reaction (PCR) method for detecting T. verrucosum. This assessment was conducted in comparison to the outcomes of conventional laboratory diagnostic tests commonly employed for trichophytosis. Methods: The research focused on analyzing 141 samples of pathological material obtained from calves in Almaty, Turkestan, and Kyzylorda regions. These calves exhibited clinical symptoms of skin disease. The study aimed to identify the causative agent using various techniques, including microscopic examination, microbiological methods involving the isolation of pure cultures, and polymerase chain reaction. Results: The detection of the causative agent of dermatophytosis using conventional methods was relatively low, 86% for the microscopic method and 79% for the microbiological method with the isolation of the culture of the pathogen. Extraction and detection of the genetic material of the causative agent of the disease for polymerase chain reaction was carried out according to the method developed by the authors. The effectiveness of the polymerase chain reaction (PCR) method was 97.9%, which is significantly higher (p<0.05) compared with the diagnostic effectiveness of conventional methods. The polymerase chain reaction method using specific primers identified the causative agent in 98% of cases, which significantly (p<0.05) exceeded the results obtained using conventional diagnostic methods. Accordingly, the PCR method had better sensitivity and specificity indicators. Conclusion: The conducted study allowed recommending the method of PCR diagnosis of dermatophytosis for fast and reliable confirmation of the diagnosis of dermatophytosis in humans and animals in Kazakhstan.

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Section: Discussionmentioning

confidence: 99%

Comparative assessment of regulated methods and PCR in the diagnosis of trichophytosis in veterinary mycology

Umitzhanov,

Abdiramanova,

Abutalip

et al. 2023

Open Vet J

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“…The development of such technologies is driven by technological considerations (such as the desired fat composition), economic factors (such as productivity), and social aspects (such as reducing the content of trans-isomers of fatty acids). The results obtained indicate that the use of such a two-stage saturation process, including non-selective hydrogenation on multifunctional catalysts of an active alloy, allows achieving the desired technological, economic, and social results in the production of edible fats from cottonseed oil (27,28) . The desired ratio of polyunsaturated fatty acids (PUFA) of the groups ω-6: ω-3 is an important aspect in determining the functionality of fat products.…”

Section: Obtaining Target Dietary Fats By Hydrogenationmentioning

confidence: 96%

Obtaining target dietary fats in the technology of step-by-step hydrogenation and their use

Sattarov

2023

Innovaciencia

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Introduction: Fats are an important component of a number of food products and affect their consistency, shelf life, taste, and nutritional qualities. Step-by-step hydrogenation is one of the main methods of modifying dietary fats, which allows for influencing their chemical structure and properties. The purpose of this study was to establish the main approaches to the step-by-step hydrogenation of dietary fats, the problems of the method, the main areas of use, and prospects for improving the technology. Material and Methods: A search to identify relevant papers was conducted for sources using open electronic databases, such as Google Scholar, Scopus, and Web of Science. Results and Discussion: During the study, the most common conditions of the hydrogenation reaction were established. The most important characteristics of the catalyst were determined by the activity, durability, selectivity, and stability of the formation of isomers. The formation of trans isomers of fatty acids, which reduces the quality of fat, has been identified as the main disadvantage of step-by-step hydrogenation. Factors contributing to the formation of trans isomers included high temperature, catalyst properties, and high content of unsaturated fatty acids in the feedstock. Step-by-step hydrogenation was identified to be the most characteristic of the soybean industry and for the production of confectionery and baking fats with specified properties. Conclusion: The results obtained indicate the potential of step-by-step hydrogenation to produce edible fats with a certain melting point and organoleptic characteristics after solving the cis/trans-isomerisation problem.

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Robust and Accurate Classification of Mutton Adulteration Under Food Additives Effect Based on Multi-Part Depth Fusion Features and Optimized Support Vector Machine

Huang

Wang

et al. 2023

Food Anal. Methods

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Meta-analysis data of the accuracy of tests for meat adulteration by real-time PCR

Cited by 8 publications

References 14 publications

Comparative assessment of regulated methods and PCR in the diagnosis of trichophytosis in veterinary mycology

Comparative assessment of regulated methods and PCR in the diagnosis of trichophytosis in veterinary mycology

Obtaining target dietary fats in the technology of step-by-step hydrogenation and their use

Robust and Accurate Classification of Mutton Adulteration Under Food Additives Effect Based on Multi-Part Depth Fusion Features and Optimized Support Vector Machine

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