Meta-Analysis and Evaluation by Insect-Mediated Baiting Reveal Different Patterns of Hypocrealean Entomopathogenic Fungi in the Soils From Two Regions of China

Masoudi, Abolfazl; Wang, Min; Zhang, Xiaoli; Wang, Can; Qiu, Zhao-Xi; Wang, Wenying; Hui, Wang; Liu, Jingze

doi:10.3389/fmicb.2020.01133

Cited by 12 publications

(15 citation statements)

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“…This study aimed to isolate entomopathogenic fungal isolates indigenous to Nile Delta as potential BCAs to control important insects attacking crops and orchards in this vital area for Egypt's food security. The insect baiting technique has been reported as a simple and effective tool for the isolation of insect associated EMPF from the soil [29,30] and DNA-based molecular characterization has become a standard approach for the accurate identification of microorganisms in this field [40]. Together they represent a pipeline for constructing collections of fungal isolates as essential step to develop microbial-based bioinsecticides as reported in numerous articles [13,41,42].…”

Section: Discussionmentioning

confidence: 99%

“…A laboratory reared strain of G. mellonella maintained at 25 ± 2 • C with 70% relative humidity (RH), a 12:12 (L:D) photoperiod and fed on an artificial diet [28] was used for entrapment of soil-associated fungi using insect baiting technique as previously described [29,30]. Briefly, collected soil samples were allowed to dry at room temperature for 3 days on a laboratory stall (to avoid infections by entomopathogenic nematodes) and pooled together after removing large debris/stones and sieving through an aperture sieve to obtain homogenous texture.…”

Section: Entrapment Of Entomopathogenic Fungi By Insect Baitingmentioning

confidence: 99%

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Screening and Identification of Indigenous Entomopathogenic Fungal Isolates from Agricultural Farmland Soils in Nile Delta, Egypt

Alfiky

2022

JoF

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The compound negative impact of insect pests attacking agricultural ecosystems includes (i) direct yield losses from damaged crops, (ii) the economic cost of the attempt to prevent these losses and (iii) the negative short- and long-term hazard effects of chemical pesticides on human and environmental health. Entomopathogenic fungi (EMPF) are a group of microorganisms that represent the natural enemies of a number of crop pests, presenting an opportunity to harness their evolutionary fine-tuned relationship with their insect hosts as biocontrol agents in integrated pest management programs. The aim of this study was to establish an indigenous EMPF collection via the Galleria mellonella (greater wax moth) entrapment method from the soils of Nile Delta, Egypt. Obtained insect associated fungal isolates were bio-assayed for pathogenicity against the serious pest Spodoptera litura and Tenebrio molitor, and the seven outperforming isolates were selected for molecular identification and thermotolerance assay. Based on ITS sequence analysis and phylogeny, selected isolates were identified as Beauveria bassiana (four isolates), Metarhizium anisopliae (two isolates) and one isolate of Cordyceps javanica. The obtained results demonstrated (i) the efficacy of using insect baiting coupled with molecular identification and pathogenicity screening to isolate EMPF to control insect pests, and (ii) the availability of indigenous virulent EMPF in Nile Delta’s soil, which can be exploited for the development of sustainable crop protection strategies.

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Section: Discussionmentioning

confidence: 99%

Section: Entrapment Of Entomopathogenic Fungi By Insect Baitingmentioning

confidence: 99%

Screening and Identification of Indigenous Entomopathogenic Fungal Isolates from Agricultural Farmland Soils in Nile Delta, Egypt

Alfiky

2022

JoF

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“…The samples were obtained using a clean spade from the root-zone soil close to the stem at a depth of 10-30 cm after removing debris. The following sampling approach was used to reduce soil heterogeneity as much as possible (Masoudi et al, 2020)…”

Section: Plot Selection and Sampling Proceduresmentioning

confidence: 99%

Plantation type and afforestation age disclose variable influences on soil microbial compositions in man‐made forests in the Xiong'an New Area, China

et al. 2022

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While afforestation helps mitigate climate concerns, the impact of plantation type and afforestation age on soil microbial compositions in afforested areas remains unclear. The current study utilized high‐throughput deep sequencing to investigate the effect of afforestation ages (3 months, 1 year and 6 months, and 15 years) and two different tree species (Pinus tabulaeformis Carr. and Sophora japonica L.) on soil microbiomes and edaphic conditions in afforested plots in the Xiong'an New Area, where soil experienced considerable disturbance by human activities during land preparation. Partial least squares path modelling showed that the afforestation age negatively influenced edaphic conditions. The results of fungal and bacterial biomass showed that bacterial lineages were replaced by fungal lineages as the afforestation age increased. Values of different soil particle sizes were impacted by increased stand ages. PERMANOVA results indicated that the plantation type could significantly explain 35.91% (p = 0.0065) and 23.84% (p = 0.009) of the bacterial and fungal β‐diversity variations, respectively. In contrast, the afforestation age could explain 35.46% (p = 0.017) of the fungal β‐diversity variation significantly. Additionally, our data showed that the co‐occurrence network for P. tabulaeformis plots was more complex than that for S. japonica plots, and network complexity increased with the afforestation age. The above results indicated that plantation type and afforestation age disclose variable influence on soil microbial compositions in man‐made forests in the Xiong'an New Area, China, which provide a basis for subsequent broader framework afforestation programs.

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“…The primers 338F/806R, 1737F/2043R, and TAReuk454FWD1F/TAReukREV3R were used to amplify the V3-V4 region of the bacterial 16S rRNA gene, fungal ITS1, and 18S rRNA, respectively, by an ABI GeneAmp® 9700 PCR thermocycler (ABI, CA, USA). PCRs, ampli cations, and sequencing were performed in the framework of our previous studies [9,11,102].…”

Section: Dna Extraction and Amplicon Sequencingmentioning

confidence: 99%

“…The time-scale sampling was selected according to the weather climate of the northern part of China. Sampling details were followed according to our previous studies [9,102]. Brie y, three random lines were randomly selected with the distance between each line at approximately 100 m, and by walking along each line, a soil core was collected every 5 m. The resulting soil cores were mixed to yield a composite sample from each line; three samples were collected per plot for each season.…”

Section: Study Area and Soil Samplingmentioning

confidence: 99%

Seasonal Variations Affect The Ecosystem Functioning And Microbial Assembly Processes In Plantation Forests

Wang¹,

Masoudi²,

Wang³

et al. 2021

Preprint

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Background: While afforestation mitigates climate concerns, the impact of afforestation on soil microbial compositions, ecological assembly processes, and multiple soil functions (multifunctionality) in afforested areas remains unclear. The Xiong'an New Area plantation forests (Pinus and Sophora forests) were selected to examine the effects of plantation types in four contrasting seasons on soil microbiomes.Results: We evaluated three functional categories (nutrient stocks, organic matter decomposition, and microbial functional genes) of multifunctionality, and the average (net) multifunctionality was quantified. The results showed that net soil multifunctionality as a broad function did not change seasonally, unlike other narrow functional categories. Bacterial communities were deterministically (variable selection and homogenous selection) structured, whereas the stochastic process of dispersal limitation was mainly responsible for the assembly and turnover of fungal and protist communities. Additionally, we showed that winter triggered an abrupt transition in the bacterial community assembly from deterministic to stochastic processes in Pinus forests that was closely associated with a reduction in the bacterial Shannon diversity, with functional patterns of a high level of nutrient cycling (nutrient stocks and organic matter decomposition). Conclusions: Overall, the present study contributes local-ecosystem prospects to model the behavior of soil biota seasonally and their implied effects on soil functioning and microbial assembly processes in plantation forests.

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Meta-Analysis and Evaluation by Insect-Mediated Baiting Reveal Different Patterns of Hypocrealean Entomopathogenic Fungi in the Soils From Two Regions of China

Cited by 12 publications

References 103 publications

Screening and Identification of Indigenous Entomopathogenic Fungal Isolates from Agricultural Farmland Soils in Nile Delta, Egypt

Screening and Identification of Indigenous Entomopathogenic Fungal Isolates from Agricultural Farmland Soils in Nile Delta, Egypt

Plantation type and afforestation age disclose variable influences on soil microbial compositions in man‐made forests in the Xiong'an New Area, China

Seasonal Variations Affect The Ecosystem Functioning And Microbial Assembly Processes In Plantation Forests

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