Messenger RNA for progesterone receptor isoforms in the late-gestation rat uterus

Fang, Xin; Wong, Sylvia C.; Mitchell, Bryan F.

doi:10.1152/ajpendo.00116.2002

Cited by 21 publications

(16 citation statements)

References 36 publications

(50 reference statements)

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“…Consistent with a previous report [6], the PR mRNA level increased during labor, and declined until day 1 of lactation. Since postpartum ovulation occurs in rats and the plasma progesterone level increases followed by the preovulatory surge in Fig.…”

Section: Discussionsupporting

confidence: 92%

“…Therefore this seems unlikely. The anti-estrogenic reagent, tamoxifen, delayed delivery and suppressed this increase in the PR mRNA level [6]. Estrogen appears to be involved in the PR mRNA increase, but in this study estrogen injection did not increase the PR mRNA level in the uterus of ovariectomized virgin rats.…”

Section: Figcontrasting

confidence: 57%

“…PR-A and PR-C are truncated forms of PR-B, and PR-A and PR-B arise from a different transcriptional start site [2]. PR mRNAs are expressed in the ovary [3,4], uterus [5,6], pituitary [7][8][9] and brain [9,10].…”

mentioning

confidence: 99%

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Progesterone Receptor mRNA Levels during Pregnancy, Labor, Lactation and the Estrous Cycle in Rat Uterus

Murata

Higuchi

2003

Journal of Reproduction and Development

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Abstract. Progesterone plays important roles in the regulation of female reproduction. In this study, progesterone receptor (PR) mRNA levels in rat uterus during pregnancy, labor, lactation and the estrous cycle were examined by competitive RT-PCR. During pregnancy and lactation, PR mRNA levels had decreased on day 20 of pregnancy (P20) and P21 compared with P15 but increased during labor. After a decline on day 1 of lactation (L1), PR mRNA levels had increased again on L3 and L14 compared with P15, P18, P20, P21 and P21pm (at 2200-2300 h on P21). There was no significant change in the PR mRNA level during the estrous cycle. The PR mRNA level did not change during 1 week of progesterone treatment or afterwards. Injection of 17β-estradiol did not affect PR mRNA levels in rats treated with progesterone or those without any injections. In rats on P18, 17 β-estradiol injection did not change PR mRNA levels after sham-operation but induced an increase in PR mRNA levels of rats ovariectomized 6 h before the treatment. These results suggest that uterine PR mRNA levels are differently regulated during late pregnancy, labor and lactation, and during labor estrogen is one of the essential factors for the increase in PR mRNA levels.

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Section: Discussionsupporting

confidence: 92%

Section: Figcontrasting

confidence: 57%

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Progesterone Receptor mRNA Levels during Pregnancy, Labor, Lactation and the Estrous Cycle in Rat Uterus

Murata

Higuchi

2003

Journal of Reproduction and Development

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“…In the mouse, loss of P 4 function at term is achieved by a decline in ovarian P 4 synthesis (Virgo & Bellward 1974), increased local metabolism of P 4 (Mahendroo et al 1996, Ishida et al 2007, and a change in expression of P 4 receptor isoforms and coactivators (Fang et al 2002, Condon et al 2003. In the human, circulating levels of P 4 do not decline before birth due to its synthesis in the placenta.…”

Section: Relevance To Cervical Remodeling In Womenmentioning

confidence: 99%

Cervical remodeling in term and preterm birth: insights from an animal model

Mahendroo¹

2012

Reproduction

174

148

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Proper cervical function is essential for a normal pregnancy and birth to occur. Understanding the mechanisms that take place in normal pregnancy will allow a better comprehension of the complications involved in premature cervical remodeling and lead to better methods of diagnostics and prevention for preterm birth. Unfortunately, human samples are not easily available, and samples that are collected are often confounded by variations in timing and region of cervix from which sample is collected. Animal models, specifically the mouse, have facilitated a great deal of exploration into the mechanisms of cervical function and pathways of preterm birth. This review highlights some of the groundbreaking discoveries that have arisen from murine research including 1) the identification of early pregnancy changes in collagen fibril processing and assembly that result in progressive modifications to collagen architecture with subsequent loss of tissue stiffness during pregnancy, 2) the determination that immune cells are not key to cervical ripening at term but have diverse phenotypes and functions in postpartum repair, and 3) the finding that the process of preterm cervical ripening can differ from term ripening and is dependent on the etiology of prematurity. These findings, which are relevant to human cervical biology, provide new insights that will allow targeted studies on the human cervix as well as identify potential biomarkers for early detection of premature cervical ripening and development of improved therapies to prevent premature ripening of the cervix and subsequent preterm birth.

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“…In rat gonadotropes or uterus where the expression of PR is up-regulated by estrogen, it has been reported that progesterone leads to a down-regulation of PR (Turgeon & Waring 2000, Fang et al 2002. However, the inhibitory effect of progesterone on PR expression was not observed in granulosa cells (Iwai et al 1991, Natraj & Richards 1993, Jo et al 2002.…”

Section: Pr In Cumulus Cells During Meiotic Resumption Of Oocytesmentioning

confidence: 99%

Expression of two progesterone receptor isoforms in cumulus cells and their roles during meiotic resumption of porcine oocytes

Shimada¹,

Yamashita²,

Ito³

et al. 2004

Journal of Molecular Endocrinology

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The present study aimed to investigate progesterone receptor (PR) gene expression in cumulus cells and their roles during meiotic resumption of porcine oocytes. The amount of PR-A or PR-B mRNA was analyzed by RT-PCR using primer sets for the PR-B region or the PR-A/B common region. The level of PR-B mRNA in cumulus cells was up-regulated by FSH and LH during the first 8 h of cultivation but the level significantly decreased at 12 h. However, a high level of total PR mRNA was maintained up to a cultivation period of 20 h. The level of PR-B protein in cumulus cells reached its maximum at 4 to 12 h, whereas PR-A predominated in cumulus cells of cumulus-oocyte complexes (COCs) at 20 h. Accompanying the shift in expression of PR isoforms, progesterone production in cumulus cells was significantly increased, and both the proliferative activity of cumulus cells during a 10-to 20-h cultivation period and the level of connexin-43, a major component of the gap junction, in cumulus cells significantly decreased. When COCs were cultured with FSH and LH for 10 h and then further cultured with additional RU486, there was a significant suppression in the shift in PR isoforms and in progesterone production, a loss of proliferative activity, and a decrease in connexin-43 mRNA in cumulus cells. Moreover, treatment with RU486 after 10-h cultivation of COCs inhibited the meiotic resumption of oocytes and cumulus cell expansion. These results suggest that the induction of PR isoforms in cumulus cells and their binding to progesterone appear to impact on proliferation and differentiation in a time-dependent manner, and the shift from PR-B to PR-A may help mediate certain events.

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Messenger RNA for progesterone receptor isoforms in the late-gestation rat uterus

Abstract: Fang, X., S. Wong, and B. F. Mitchell. Messenger RNA for progesterone receptor isoforms in the late-gestation rat uterus.

Cited by 21 publications

References 36 publications

Progesterone Receptor mRNA Levels during Pregnancy, Labor, Lactation and the Estrous Cycle in Rat Uterus

Progesterone Receptor mRNA Levels during Pregnancy, Labor, Lactation and the Estrous Cycle in Rat Uterus

Cervical remodeling in term and preterm birth: insights from an animal model

Expression of two progesterone receptor isoforms in cumulus cells and their roles during meiotic resumption of porcine oocytes

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