Messenger RNA expression profiles and bioinformatics analysis of mouse hippocampi during exercise alleviates methamphetamine dependence via mRNA profile change in hippocampi

Li, Yue; Re, Guo-Fen; Zhao, Yu; Kong, Deshenyue; Mao, Jun-Hong; Wang, Kunhua; Kuang, Yi‐Qun

doi:10.21037/atm-22-450

Cited by 4 publications

(4 citation statements)

References 37 publications

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“…Moreover, we additionally found that METH markedly disturbed the expression of the genes associated with cell cycle progression, which resulted in cell cycle arrest and eventually led to abnormalities in both differentiation and migration of neurospheres. Although the screened DEGs in this study were differently changed compared to the reported DEGs in METH-treated hippocampus in vivo ( Zoubková et al, 2019 ; Li et al, 2022 ), the identified DEGs in neurospheres were not only validated by the qRT-PCR but the DEGs-enriched cell signaling was also investigated, demonstrating the critical role of altered genes in METH-caused toxicity. Hence, our findings shed light on the mechanisms underlying METH-induced neurodevelopmental deficits.…”

Section: Discussionmentioning

confidence: 64%

Methamphetamine exposure drives cell cycle exit and aberrant differentiation in rat hippocampal-derived neurospheres

Wang,

et al. 2023

Front. Pharmacol.

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Introduction: Methamphetamine (METH) abuse by pregnant drug addicts causes toxic effects on fetal neurodevelopment; however, the mechanism underlying such effect of METH is poorly understood.Methods: In the present study, we applied three-dimensional (3D) neurospheres derived from the embryonic rat hippocampal tissue to investigate the effect of METH on neurodevelopment. Through the combination of whole genome transcriptional analyses, the involved cell signalings were identified and investigated.Results: We found that METH treatment for 24 h significantly and concentration-dependently reduced the size of neurospheres. Analyses of genome-wide transcriptomic profiles found that those down-regulated differentially expressed genes (DEGs) upon METH exposure were remarkably enriched in the cell cycle progression. By measuring the cell cycle and the expression of cell cycle-related checkpoint proteins, we found that METH exposure significantly elevated the percentage of G0/G1 phase and decreased the levels of the proteins involved in the G1/S transition, indicating G0/G1 cell cycle arrest. Furthermore, during the early neurodevelopment stage of neurospheres, METH caused aberrant cell differentiation both in the neurons and astrocytes, and attenuated migration ability of neurospheres accompanied by increased oxidative stress and apoptosis.Conclusion: Our findings reveal that METH induces an aberrant cell cycle arrest and neuronal differentiation, impairing the coordination of migration and differentiation of neurospheres.

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Section: Discussionmentioning

confidence: 64%

Methamphetamine exposure drives cell cycle exit and aberrant differentiation in rat hippocampal-derived neurospheres

Wang,

et al. 2023

Front. Pharmacol.

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“…In another study, Li et al demonstrate that mice subjected to treadmill running have reduced addiction to methamphetamine. Using RNA‐seq, they show that the a number of genes are differentially regulated between running and sedentary mice given methamphetamine (Li et al, 2022).…”

Section: Molecular Mechanisms Underlying Enhanced Adult Hippocampal N...mentioning

confidence: 99%

“…In another study,Li et al demonstrate that mice subjected to treadmill running have reduced addiction to methamphetamine. Using RNA-seq, they show that the a number of genes are differentially regulated between running and sedentary mice given methamphetamine(Li et al, 2022).McGreevy et al have investigated the heritability of the impact of physical exercise from father to offspring. They show that increased adult neurogenesis, greater mitochondrial citrate synthase activity, and paternal cognitive improvements of the runner-fathers can be inherited by their offspring.…”

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confidence: 99%

Mechanisms underlying the effect of voluntary running on adult hippocampal neurogenesis

2023

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Adult hippocampal neurogenesis is important for preserving learning and memoryrelated cognitive functions. Physical exercise, especially voluntary running, is one of the strongest stimuli to promote neurogenesis and has beneficial effects on cognitive functions. Voluntary running promotes exit of neural stem cells (NSCs) from the quiescent stage, proliferation of NSCs and progenitors, survival of newborn cells, morphological development of immature neuron, and integration of new neurons into the hippocampal circuitry. However, the detailed mechanisms driving these changes remain unclear. In this review, we will summarize current knowledge with respect to molecular mechanisms underlying voluntary running-induced neurogenesis, highlighting recent genome-wide gene expression analyses. In addition, we will discuss new approaches and future directions for dissecting the complex cellular mechanisms driving change in adult-born new neurons in response to physical exercise.

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“…There is extensive work in the literature ( Centanni et al, 2022 ; Li et al, 2022 ; Re et al, 2022 ) demonstrating that exercise can improve withdrawal symptoms and reduce relapse rates. Therefore, exercise has been considered an excellent adjunct treatment to existing treatment regimens ( Wang et al, 2014 ; He et al, 2021 ; Salem et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Effects of exercise of different intensities on withdrawal symptoms among people with substance use disorder: a systematic review and meta-analysis

Cai

et al. 2023

Front. Physiol.

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Background: Exercise can effectively attenuate withdrawal symptoms and reduce relapse, but it is unknown whether exercise of different intensities produces different results. This study aimed to systematically review the effects of different exercise intensities on withdrawal symptoms among people with substance use disorder (SUD).Methods: Systematic searches for randomized controlled trials (RCTs) on exercise, SUD, and abstinence symptoms were conducted via electronic databases, including PubMed, up to June 2022. Study quality was evaluated using the Cochrane Risk of Bias tool (RoB 2.0) for assessment of risk of bias in randomized trials. The meta-analysis was performed by calculating the standard mean difference (SMD) in outcomes of interventions involving light-, moderate-, and high-intensity exercise for each individual study using Review Manager version 5.3 (RevMan 5.3).Results: In total, 22 RCTs (n = 1,537) were included. Overall, exercise interventions had significant effects on withdrawal symptoms, but the effect size varied with exercise intensity and by outcome measure (i.e., for different negative emotions). Light-, moderate-, and high-intensity exercise reduced cravings after the intervention [SMD = −0.71, 95% CI = (−0.90, −0.52)], and there were no statistical differences between the subgroups (p > 0.05). Light-, moderate-, and high-intensity exercise reduced depression after the intervention [light, SMD = −0.33, 95% CI = (−0.57, −0.09); moderate, SMD = −0.64, 95% CI = (−0.85, −0.42); high, SMD = −0.25, 95% CI = (−0.44, −0.05)], with moderate-intensity exercise producing the best effect (p < 0.05). Only light- and moderate-intensity exercise relieved anxiety after the intervention [light, SMD = −0.48, 95% CI = (−0.71, −0.26); moderate, SMD = −0.58, 95% CI = (−0.85, −0.31)]. Only high-intensity exercise worked in alleviating stress [SMD = −1.13, 95% CI = (−2.22, −0.04)]. Both irritability and restlessness could be improved by light- and moderate-intensity exercise [irritability, SMD = −0.74, 95% CI = (−0.98, −0.50); restless, SMD = −0.72, 95% CI = (−0.98, −0.47)], and there were no statistical differences between the subgroups (p > 0.05). Moderate- and high-intensity exercise decreased withdrawal syndrome after the intervention [moderate, SMD = −0.30, 95% CI = (−0.55, −0.05); high, SMD = −1.33, 95% CI = (−1.90, −0.76)], with high-intensity exercise producing the best effects (p < 0.01).Conclusion: Overall, exercise leads to improvements in withdrawal symptoms in individuals with SUD, but these effects vary significantly between the exercise of different intensities and according to the type of withdrawal symptoms. Moderate-intensity exercise has the greatest benefits in improving depression and anxiety; high-intensity exercise has the greatest benefits in improving withdrawal syndrome.Systematic Review Registration:www.crd.york.ac.uk/PROSPERO/, identifier, CRD42022343791

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Messenger RNA expression profiles and bioinformatics analysis of mouse hippocampi during exercise alleviates methamphetamine dependence via mRNA profile change in hippocampi

Cited by 4 publications

References 37 publications

Methamphetamine exposure drives cell cycle exit and aberrant differentiation in rat hippocampal-derived neurospheres

Methamphetamine exposure drives cell cycle exit and aberrant differentiation in rat hippocampal-derived neurospheres

Mechanisms underlying the effect of voluntary running on adult hippocampal neurogenesis

Effects of exercise of different intensities on withdrawal symptoms among people with substance use disorder: a systematic review and meta-analysis

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