messenger RNA-containing ribonucleoprotein from mitochondrial polyribosomes of rat liver

Kisselev, O.I.; Gaitskhoki, B. S.; Klimov, N. A.

doi:10.1007/bf00357545

Cited by 6 publications

(4 citation statements)

References 23 publications

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“…These results provide definitive evidence for the existence of discrete po!y(A)-lacking mRNAs in Ehrlich ascites mitochondria. In keeping with our previous (Avadhani et al, 1974) and present findings, Kisselev et al (1975) have recently shown that only about 30% of RNA released from rat liver mt polysomes bind to oligo(dT)-cellulose implying that the rest of the mRNAs may lack poly(A).…”

Section: Discussionsupporting

confidence: 93%

Messenger RNA metablism in mammalian mitochondria. IV. Messenger ribonucleic acid metabolism in mammalian mitochondria. Discrete poly(adenylic acid) lacking messenger ribonucleic acid species associated with mitochondrial polysomes

Lewis¹,

Rutman²,

Avadhani³

1976

Biochemistry

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The mRNA species released from mitochondrial polysomes prepared by the Mg2+ precipitation technique have been further characterized using various analytical techniques. Mitochondrial polysomes were dissociated by treatment with puromycin and chemically labeled with (3H) dimethyl sulfate. About 51% of steady-state mitochondrial mRNA bind to oligo(dT)-cellulose indicating the presence of poly(adenylic acid)(poly(A)) in this fraction. The poly(A)-containing mRNAs resolve into discrete bands of 9-16 Se, while the RNA fraction unable to bind to oligo(dT)-cellulose representing poly(A)-lacking mRNA contains 8-12 Se species. About 90% of poly(A) lacking RNA hybridizes with mitochondrial DNA and less than 7% hybridizes with nuclear DNA. The extent of hybridization of poly(A)-lacking RNA with mitochondrial DNA was not significantly affected by the presence of excess mitochondrial rRNA, cytoplasmic rRNA, or a tenfold concentration of poly(A)-containing RNA isolated from total mitochondrial RNA. Possible differences in sequence properties between poly(A)-containing and -lacking mitochondrial mRNAs were further verified using a solid phase-bound cDNA procedure. Poly(A)-containing mRNA released from mitochondrial polysomes shows over 85% sequance homology with oligo(dT)-cellulose-bound cDNA prepared against total mitochondrial poly(A)-lacking mitochondrial mRNA hybridizes with the cDNA providing direct evidence for the distinct sequence properties of the two mRNA species.

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Section: Discussionsupporting

confidence: 93%

Messenger RNA metablism in mammalian mitochondria. IV. Messenger ribonucleic acid metabolism in mammalian mitochondria. Discrete poly(adenylic acid) lacking messenger ribonucleic acid species associated with mitochondrial polysomes

Lewis¹,

Rutman²,

Avadhani³

1976

Biochemistry

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“…Polysomes Qf eukaryotic cells can be dissociated in various ways: in vivo by incubating cells with puromycin (2,3,4), in aminoacid depleted media (5), at elevated temperature (6), under hypertonic conditions (7,8), in the presence of dimethyl sulfoxide (9), in vitro by incubating polysomes with EDTA (10,11,12,13,14,15), under conditions where ribosomes run off the mRNA without reinitiation (16,17), with puromycin at high salt concentration (18,19,20). Wherever it has been tested such dissociations of polysomes have been shown to release mRNA in the form of ribonucleoprotein particles.…”

Section: Introductionmentioning

confidence: 99%

Characterization of mRNA-protein complexes from mammalian cells

Sundquist¹,

Persson²,

Lindberg³

1977

Nucl Acids Res

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In a previous report we described the use of oligo(dT)-cellulose for the isolation of mRNA-protein complexes from EDTA-dissociated polysomes extracted from normally growing or adenovirus infected KB-cells (I). Experiments presented here provide evidence that proteins involved in these complexes bind specifically to mRNA since: a) the proteins and mRNA cosediment through sucrose gradients, b) they adsorb and elute from oligo(dT)-cellulose together, and c) analysis of the products from ribonuclease digestion experiments show that the poly (A) end and a separate small fraction of the mRNA are resistant to the enzymes and attached to protein.

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“…Isolation of mitochondria. 1iitochondria were isolated as described (10) and treated with digitonin to remove cytoplasmic contaminations (11).…”

Section: Materiai3 and Methodsmentioning

confidence: 99%

Isolation and partial characterization of poly (A)-containing 7.5S messenger RNA from rat liver mitochondria

1977

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Poly(A)-containing low molecular weight (7.5S) messenger RNA was isolated in a highly purified form from both polyribosomes and post-polysomal supernatant of rat liver mitochondria. Both mRNA's contain rather short poly(A) tracts (40-70 mononucleotides) according to a profile of their elution from poly(U)-Sepharose column with a gradient of formamide concentration. Both mRNA's when added to a preincubated mitochondrial lysate programmed the synthesis of a hydrophobic polypeptide of a molecular weight about 9000 daltons which was soluble in the neutral chloroform-methanol mixture.

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messenger RNA-containing ribonucleoprotein from mitochondrial polyribosomes of rat liver

Cited by 6 publications

References 23 publications

Messenger RNA metablism in mammalian mitochondria. IV. Messenger ribonucleic acid metabolism in mammalian mitochondria. Discrete poly(adenylic acid) lacking messenger ribonucleic acid species associated with mitochondrial polysomes

Messenger RNA metablism in mammalian mitochondria. IV. Messenger ribonucleic acid metabolism in mammalian mitochondria. Discrete poly(adenylic acid) lacking messenger ribonucleic acid species associated with mitochondrial polysomes

Characterization of mRNA-protein complexes from mammalian cells

Isolation and partial characterization of poly (A)-containing 7.5S messenger RNA from rat liver mitochondria

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