Mesquite gum: fractionation and characterization of the gum exuded from Prosopis laevigata obtained from plant tissue culture and from wild trees

Orozco-Villafuerte, J.; Cruz-Sosa, Francisco; Ponce‐Alquicira, Edith; Vernon‐Carter, E.J.

doi:10.1016/s0144-8617(03)00187-5

Cited by 54 publications

(24 citation statements)

References 26 publications

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“…laevigata is known to contribute gum exudates, which contain several amino acids, carbohydrates [sacarose, glucosidated flavonoids, pipecolic acids, and pinitol (poly-alcohol)], and this may be one of the main effects that contributes to changes in soil biota composition together with the shedding of leaves Harbone and Baxter 1993;Orozco-Villafuerte et al 2003;Garcia-Bores 2003).…”

Section: Discussionmentioning

confidence: 99%

The effect ofCercidium praecoxandProsopis laevigataon vertical distribution of soil free‐living nematode communities in the Tehuacán Desert, Mexico

Pen-Mouratov¹,

Rodrı́guez-Zaragoza²,

Steinberger³

2008

Ecological Research

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Vegetation cover is known to act as an abiotic mediator influencing the structure of soil fauna communities in arid and semi-arid ecosystems. The aim of the current research was to determine the spatial dispersion of the soil free-living nematode community under the canopy of Cercidium praecox and Prosopis laevigata during the rainy season. These shrubs are the dominant plant associations in the western part of the Tehuaca´n-Cuicatla´n Valley in Mexico. Soil samples were taken from each 10-cm depth between 0 and 50 cm in August 2004. Our results demonstrated that the abundance and structure of the soil free-living nematode communities in the study area were strongly dependent on plant effects, specified by limited factors such as soil moisture and organic matter availability. The greatest degree of abundance of soil-free-living nematodes (88%) was found in the upper (0-10 cm) soil layer. Plant parasites were the most abundant trophic group under the two plants (58 and 36% under Parkinsonia (Cercidium) praecox and Prosopis laevigata, respectively), whereas omnivore-predators were the most dominant (96%) in inter-plant spaces. The fungivore/bacterivore (F/B) ratio was found to be the most useful tool of the ecological indices tested in the present study, reflecting the vertical distribution of the freeliving nematode communities beneath different plant species in the different soil layers. The soil free-living nematode communities and their vertical distribution were found to be affected by plant ecophysiological adaptation, soil moisture, and the interaction between them.

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Section: Discussionmentioning

confidence: 99%

The effect ofCercidium praecoxandProsopis laevigataon vertical distribution of soil free‐living nematode communities in the Tehuacán Desert, Mexico

Pen-Mouratov¹,

Rodrı́guez-Zaragoza²,

Steinberger³

2008

Ecological Research

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“…Furthermore, this species has great ecological value because it helps to control erosion and to improve soil fertility (Stanton et al 2001). One of the most important aspects of P. laevigata, which provides it with an added value, is that it produces a gum that has similar chemical composition (Orozco-Villafuerte et al 2003) and physicochemical properties as those exhibited by gum arabic, which is considered as a benchmark emulsifying agent in the food and other industries (Garti and Reichmann, 1993;VernonCarter et al, 2000). However, misuse of this resource has caused deforestation and irreversible loss of genetic diversity combined to a high intraspecific variability in the mesquite gum production.…”

Section: Introductionmentioning

confidence: 99%

Clonal propagation of mesquite tree (Prosopis laevigata Humb. & Bonpl. ex Willd. M.C. Johnston). I. Via cotyledonary nodes

Buendía-González

Orozco-Villafuerte

Cruz-Sosa

et al. 2007

In Vitro Cell.Dev.Biol.-Plant

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“…Around 10 g of gum-like substance produced after the fourth callus subculture, were collected from the surface of the calluses with the help of a Pasteur pipette. All the samples were dissolved in 200 mL of deionized water, filtered through nitrocellulose membrane (0.45 lM), separated by dialysis against running tap deionized water for 48 h (molecular weight cut-off 12 kDa), and freeze-dried (Orozco-Villafuerte et al 2003).…”

Section: Gum Purificationmentioning

confidence: 99%

“…Orozco-Villafuerte et al (2003 showed the feasibility of in vitro mesquite gum production using stem segments of P. laevigata and demonstrated that the gum synthesis is stimulated by biotic (suspensions of dry bacterium cells) and abiotic (mechanical wounding and temperature) stress. In addition, they proved that the physicochemical characteristics of in vitro mesquite gum were very close to those of the gum produced by the wild trees.…”

Section: Introductionmentioning

confidence: 99%

Establishment and characterization of Prosopis laevigata (Humb. & Bonpl. ex Willd) M.C. Johnst. cell suspension culture: a biotechnology approach for mesquite gum production

et al. 2011

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This study presents a protocol for the establishment of Prosopis laevigata cell suspension culture as a strategy to obtain an in vitro mesquite gum productive cell line. The callus used for this purpose was obtained with hypocotyls from 15-day-old plantlets, placed on Murashige-Skoog medium with two different plant growth regulators (PGRs), 2,4,5-trichlorophenoxy acetic acid (2,4,5-T; 5.0 lM) and kinetin (KIN; 5.0 lM). With this PGRs treatment, after four subcultures (30 days each) an exuded gum-like substance was observed on the callus surface. The growth kinetics of the cell suspension culture showed a specific cell growth rate (l) of 0.14 d -1 and doubling time (t d ) of 6.6 days, respectively. The gum-like substance from callus culture and the broth from cell suspension culture were subjected to chemical analysis and compared with the mesquite gum exuded from wild trees. Both, gum-like substance from callus culture and the broth from cell suspension culture showed the presence of Arabinogalactanproteins, and their polysaccharide fraction presented the same monosaccharides as those isolated from mesquite gum. In addition, the emulsifying properties of gum-like substance from callus culture and the broth from cell suspension culture were compared to those of mesquite gum and all three samples exhibited similar emulsifying capacity and emulsification stability.

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Mesquite gum: fractionation and characterization of the gum exuded from Prosopis laevigata obtained from plant tissue culture and from wild trees

Cited by 54 publications

References 26 publications

The effect ofCercidium praecoxandProsopis laevigataon vertical distribution of soil free‐living nematode communities in the Tehuacán Desert, Mexico

The effect ofCercidium praecoxandProsopis laevigataon vertical distribution of soil free‐living nematode communities in the Tehuacán Desert, Mexico

Clonal propagation of mesquite tree (Prosopis laevigata Humb. & Bonpl. ex Willd. M.C. Johnston). I. Via cotyledonary nodes

Establishment and characterization of Prosopis laevigata (Humb. & Bonpl. ex Willd) M.C. Johnst. cell suspension culture: a biotechnology approach for mesquite gum production

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