Mesothelial cells in tissue repair and fibrosis

Mutsaers, Steven E.; Birnie, Kimberly A; Lansley, Sally M.; Herrick, Sarah E.; Lim, Chuan Bian; Prêle, Cecilia M.

doi:10.3389/fphar.2015.00113

Cited by 170 publications

(177 citation statements)

References 198 publications

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“…The mesothelium is a slowly renewing tissue with 0.16-0.5% of cells undergoing mitosis at any one time [14]. The primary function of the mesothelium is to provide a slippery surface, but it is also involved in the transport of solutes and cells, immune defenses and tissue regeneration [14].…”

Section: The Ultrastructure Of the Pericardiummentioning

confidence: 99%

“…The primary function of the mesothelium is to provide a slippery surface, but it is also involved in the transport of solutes and cells, immune defenses and tissue regeneration [14].…”

Section: The Ultrastructure Of the Pericardiummentioning

confidence: 99%

“…The exposed basal lamina could be also repopulated by www.fhc.viamedica.pl cells free-floating in the pericardial fluid [19]. The mesothelial repair is different from that occurring in other epithelia, as regrowth appears diffusely across the injured surface [14]. After the return to primary intact constitution, the cells undergo transformation to mesothelial-like phenotype [18,19].…”

Section: Acute Inflammation and Tissue Repairmentioning

confidence: 99%

“…Proposed events leading to chronic inflammation of serosal membranes including peritoneum, are extrinsic factors such as infections, surgical manipulation of the pericardium, bleeding to the pericardial space, extracorporeal circulation, cardiac failure and uremia, and intrinsic factors such as the loss of mesothelial cells and exposure of the submesothelial layer, EMT, abundance of macrophages, and decreased fibrinolytic activity of mesothelial cells, observed also in peritoneum and pleura [14,20,21].…”

Section: Chronic Inflammation and Intrapericardial Adhesionsmentioning

confidence: 99%

“…Secretion of chemokines by mesothelial cells promotes trans-mesothelial migration of neutrophils and monocytes/ /macrophages into the serosal space [18,23]. This is facilitated by the expression of integrins and adhesion molecules on mesothelium (ICAM-1) and leukocytes (LFA-1, Mac-1) [14,24]. However, mesothelial cells also participate in the resolution of inflammation by specific integrin-mediated regulation involving very late antigens (VLA-4 and VLA-5) expression [14].…”

Section: Chronic Inflammation and Intrapericardial Adhesionsmentioning

confidence: 99%

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Pericardium: structure and function in health and disease

Jaworska-Wilczyńska

Trzaskoma

Szczepankiewicz

et al. 2016

Folia Histochem Cytobiol.

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Normal pericardium consists of an outer sac called fibrous pericardium and an inner one called serous pericardium. The two layers of serous pericardium: visceral and parietal are separated by the pericardial cavity, which contains 20 to 60 mL of the plasma ultrafiltrate. The pericardium acts as mechanical protection for the heart and big vessels, and a lubrication to reduce friction between the heart and the surrounding structures. A very important role in all aspects of pericardial functions is played by mesothelial cells. The mesothelial cells form a monolayer lining the serosal cavity and play an important role in antigen presentation, inflammation and tissue repair, coagulation and fibrinolysis. The two major types of mesothelial cells, flat or cuboid, differ substantially in their ultrastructure and, probably, functions. The latter display abundant microvilli, RER, Golgi dense bodies, membrane-bound vesicles and intracellular vacuoles containing electron-dense material described as dense bodies. The normal structure and functions of the pericardium determine correct healing after its injury as a result of surgery or microbial infection. The unfavorable resolution of acute or chronic pericarditis leads to the formation of adhesions between pericardial leaflets which may lead to serious complications.

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Section: The Ultrastructure Of the Pericardiummentioning

confidence: 99%

“…The primary function of the mesothelium is to provide a slippery surface, but it is also involved in the transport of solutes and cells, immune defenses and tissue regeneration [14].…”

Section: The Ultrastructure Of the Pericardiummentioning

confidence: 99%

Section: Acute Inflammation and Tissue Repairmentioning

confidence: 99%

Section: Chronic Inflammation and Intrapericardial Adhesionsmentioning

confidence: 99%

Section: Chronic Inflammation and Intrapericardial Adhesionsmentioning

confidence: 99%

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Pericardium: structure and function in health and disease

Jaworska-Wilczyńska

Trzaskoma

Szczepankiewicz

et al. 2016

Folia Histochem Cytobiol.

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Upregulation of mesothelial genes in ovarian carcinoma cells is associated with an unfavorable clinical outcome and the promotion of cancer cell adhesion

et al. 2020

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A hallmark of ovarian high‐grade serous carcinoma (HGSC) is its early and massive peritoneal dissemination via the peritoneal fluid. It is generally believed that tumor cells must breach the mesothelium of peritoneal organs to adhere to the underlying extracellular matrix (ECM) and initiate metastatic growth. However, the molecular mechanisms underlying these processes are only partially understood. Here, we have analyzed 52 matched samples of spheroids and solid tumor masses (suspected primary lesions and metastases) from 10 patients by targeted sequencing of 21 loci previously proposed as targets of HGSC driver mutations. This analysis revealed very similar patterns of genetic alterations in all samples. One exception was FAT3 with a strong enrichment of mutations in metastases compared with presumed primary lesions in two cases. FAT3 is a putative tumor suppressor gene that codes for an atypical cadherin, pointing a potential role in peritoneal dissemination in a subgroup of HGSC patients. By contrast, transcriptome data revealed clear and consistent differences between tumor cell spheroids from ascites and metastatic lesions, which were mirrored by the in vitro adherence of ascites‐derived spheroids. The adhesion‐induced transcriptional alterations in metastases and adherent cells resembled epithelial–mesenchymal transition, but surprisingly also included the upregulation of a specific subset of mesothelial genes, such as calretinin (CALB2) and podoplanin (PDPN). Consistent with this finding, calretinin staining was also observed in subsets of tumor cells in HGSC metastases, particularly at the invasive tumor edges. Intriguingly, a high expression of either CALB2 or PDPN was strongly associated with a poor clinical outcome. siRNA‐mediated CALB2 silencing triggered the detachment of adherent HGSC cells in vitro and inhibited the adhesion of detached HGSC cells to collagen type I. Our data suggest that the acquisition of a mesenchymal–mesothelial phenotype contributes to cancer cell adhesion to the ECM of peritoneal organs and HGSC progression.

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Laboratory Techniques for Fluid Analysis

Vap¹,

Sprague²

2020

Veterinary Cytology

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Mesothelial cells in tissue repair and fibrosis

Cited by 170 publications

References 198 publications

Pericardium: structure and function in health and disease

Pericardium: structure and function in health and disease

Upregulation of mesothelial genes in ovarian carcinoma cells is associated with an unfavorable clinical outcome and the promotion of cancer cell adhesion

Laboratory Techniques for Fluid Analysis

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