Mesothelial cell proliferation: a nonspecific response to lung injury associated with fibrosis.

Adamson, I. Y. R.; Bąkowska, Julita; Bowden, D. H.

doi:10.1165/ajrcmb.10.3.7509611

Cited by 42 publications

(24 citation statements)

References 11 publications

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“…reported that the peak blood level of early mesothelial proliferation occurred in the first 2-4 weeks after asbestos exposure [20]. However, there was no study of early pleural reaction after pleurodesis using imaging modality.…”

Section: Resultsmentioning

confidence: 99%

Ex vivo High-resolution Optical Coherence Tomography (OCT) Imaging of Pleural Reaction after Pleurodesis Using Talc

Ahn

Oak

Park

et al. 2016

Journal of the Optical Society of Korea

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The pleura is known as an end target organ of exposure to toxic environmental materials such as fine particulate matter and asbestos. Moreover, long-term exposure to hazardous materials can eventually lead to fatal lung disease such as diffuse pleural fibrosis or mesothelioma. Chest computed tomography (CT) and ultrasound are gold standard imaging modalities for detection of advanced pleural disease. However, a diagnostic tool for early detection of pleural reaction has not been developed yet due to difficulties in imaging ultra-fine structure of the pleura. Optical coherence tomography (OCT), which provides cross-sectional images of micro tissue structures at a resolution of 2-10 μm, can image the mesothelium with a thickness of ~100 μm and therefore enables investigation of the early pleural reaction. In this study, we induced the early pleural reaction according to a time sequence after pleurodesis using talc, which has been widely used in the clinical field. The pleural reaction in talc grouped according to the time sequence (1st, 2nd, 4th weeks) showed a significant thickening (average thickness: 45 ± 7.5 μm, 80 ± 10.7 μm, 90 ± 12.5 μm), while the pleural reaction in sham and normal groups showed pleural change from normal to minimal thickening (average thickness: 16 ± 5.5 μm, 17 ± 4.5 μm, 15 ± 6.5 μm, and 12 ± 7.5 μm, 13 ± 2.5 μm, 12 ± 3.5 μm). The measurement of pleural reaction by pathologic examinations was well-matched with the measurement by OCT images. This is the first study for measuring the thickness of pleural reactions using a biophotonic modality such as OCT. Our results showed that OCT can be useful for evaluating the early pleural reaction.

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Section: Resultsmentioning

confidence: 99%

Ex vivo High-resolution Optical Coherence Tomography (OCT) Imaging of Pleural Reaction after Pleurodesis Using Talc

Ahn

Oak

Park

et al. 2016

Journal of the Optical Society of Korea

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“…Subsequent study revealed that this proliferation could result as a nonspecific pleural change following fibrogenic insult to the lung (13). Several authors have suggested that this asbestos-induced visceral pleural mesothelial cell proliferation does not represent a direct effect of fibers in contact with mesothelial cells but may be due to fiber-induced release of reactive oxygen species, cytokines, or growth factors that stimulate cell proliferation in these cells (13,14). These conclusions were made because light microscopic examination of lung and pleura sections found no fibers.…”

Section: Discussionmentioning

confidence: 99%

Comparison of pleural responses of rats and hamsters to subchronic inhalation of refractory ceramic fibers.

Everitt

Gelzleichter

Bermudez

et al. 1997

Environ Health Perspect

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In the present subchronic study, we compared pleural inflammation, visceral pleural collagen deposition, and visceral and parietal pleural mesothelial cell proliferation in rats and hamsters identically exposed to a kaolin-based refractory ceramic fiber, (RCF)-1 by nose-only inhalation exposure, and correlated the results to translocation of fibers to the pleural cavity. Fischer 344 rats and Syrian golden hamsters were exposed to 650 fibers/cc of RCF-1, for 4 hr/day, 5 days/week for 12 weeks. Following 4 and 12 weeks of exposure, and after a 12-week recovery period, pleural lavage fluid was analyzed for cytologic and biochemical evidence of inflammation. Visceral and parietal pleural mesothelial cell proliferation was assessed by immunocytochemical detection of bromodeoxyuridine incorporation. Pleural collagen was quantitated using morphometric analysis of lung sections stained with Sirius Red. Fiber-exposed rats and hamsters had qualitatively similar pleural inflammation at each time point. Mesothelial cell proliferation was more pronounced in hamsters than in rats at each time point and at each site. In both species, the mesothelial cell labeling index was highest in the parietal pleural mesothelial cells lining the surface of the diaphragm at each time point. Hamsters but not rats had significantly elevated collagen in the visceral pleura at the 12-week postexposure time point. Fibers were found in the pleural cavities of both species at each time point. These fibers were generally short and thin. These results suggest that mesothelial cell proliferation and fibroproliferative changes in the pleura of rodents following short-term inhalation exposure are associated with fiber translocation to the pleura and may be predictive of chronic pleural disease outcomes following long-term exposure.

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“…The MC were seeded in 24-well plates at 2 x 104 cells per well in DMEM containing 0.5% FBS. In sets of four wells, AM and PLM supernatants from exposed and control rats, and BALF and PLLF from the same rats (see above) were added at 10 and 25% by volume. The lavage fluids were concentrated 4-fold by centrifugation using a microconcentrating membrane with a 30-kD cutoff.…”

Section: In Vitro Experimentsmentioning

confidence: 99%

Early mesothelial cell proliferation after asbestos exposure: in vivo and in vitro studies.

Adamson

1997

Environ Health Perspect

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There is some evidence that proliferation of pleural mesothelial cells (MC) occurs soon after deposition of asbestos fibers. To study this effect, we instilled a single dose of 0.1 mg crocidolite into the lungs of mice for 1 and 6 weeks and counted labeled nuclei after 3H-thymidine (3HTI) injection. Short fibers (<1 pm) induced little change in the lung; they were mostly phagocytized and had a minimal effect on MC labeling. Long fibers up to 20 pm damaged the bronchiolar epithelium and were incorporated into connective tissue. Increased 3HT uptake was seen in fibroblasts and epithelial cells and also in MC, which peaked at 2% labeled at 1 week compared to near 0% labeling in controls. No fibers were found in or near labeled MC, which suggested that a cytokine generated in the lung during the early response phase might induce MC proliferation. To look for a cytokine effect in vitro, we instilled asbestos into rat lungs and, after 1 and 6 weeks, bronchoalveolar and pleural lavage fluids as well as macrophages were collected. Alveolar macrophages contained fibers, but pleural macrophages (PM) did not. After short-term culture, macrophage supernatants and the lavage fluids were tested on rat lung MC in culture. At 1 week, PM secreted growth factor(s) for MC, and the mitogenic effect was more pronounced with lavage fluids. No effects on MC were found using material prepared 6 weeks after asbestos. The early MC growth increase was not blocked by antibodies to cytokines, such as plateletderived growth factor, fibroblast growth factors, or tumor necrosis factor, but was inhibited by anti-keratinocyte growth factor (anti-KGF). The results show that an early growth phase of MC after asbestos exposure appears unrelated to particle translocation to the pleura but is associated with cytokine release, most likely KGF, by lung cells.

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Mesothelial cell proliferation: a nonspecific response to lung injury associated with fibrosis.

Cited by 42 publications

References 11 publications

Ex vivo High-resolution Optical Coherence Tomography (OCT) Imaging of Pleural Reaction after Pleurodesis Using Talc

Ex vivo High-resolution Optical Coherence Tomography (OCT) Imaging of Pleural Reaction after Pleurodesis Using Talc

Comparison of pleural responses of rats and hamsters to subchronic inhalation of refractory ceramic fibers.

Early mesothelial cell proliferation after asbestos exposure: in vivo and in vitro studies.

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