Mesoscopic in vivo 3-D tracking of sparse cell populations using angular multiplexed optical projection tomography

Alexandrov, Yuriy; Kumar, Sunil; Andrews, Natalie; Dallman, Margaret J.; French, Paul M. W.; McGinty, James

doi:10.1364/boe.6.001253

Cited by 6 publications

(3 citation statements)

References 17 publications

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“…The first commercial devices were made available and an open source, custom built-version named OpenSPIM was produced [64,65]. OPT and/or LSFM have been used to image multicellular culture models [66][67][68][69], zebrafish [70], sparse cell populations [71], the development of plants [72], living embryos and gene mapping [58,73], as well as multiple rodent organs in health and disease conditions [50,53,74,75]. OPT and LSFM were both successfully used to obtain detailed insight of the anatomy of the flight musculature of a Drosophila fly, its nervous and digestive systems, and ß-galactoside activity at whole-body level [76,77].…”

Section: Optical Projection Tomography (Opt) and Selective Plane Illumentioning

confidence: 99%

Toolbox for In Vivo Imaging of Host–Parasite Interactions at Multiple Scales

Niz

Spadin

Marti

et al. 2019

Trends in Parasitology

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Animal models have for long been pivotal for parasitology research. Over the last few years, techniques such as intravital, optoacoustic and magnetic resonance imaging, optical projection tomography, and selective plane illumination microscopy developed promising potential for gaining insights into host-pathogen interactions by allowing different visualization forms in vivo and ex vivo. Advances including increased resolution, penetration depth, and acquisition speed, together with more complex image analysis methods facilitate tackling biological problems previously impossible to study and/or quantify. Here we discuss advances and challenges in the in vivo imaging toolbox, which hold important potential for the field of parasitology. KeywordsParasitology, imaging, in vivo, animal models Imaging toolbox in parasitologyImaging techniques developed for biomedical applications have had an important impact in parasitology research. Such techniques include platforms developed to image host-pathogen interactions at various scales, ranging from molecules to whole organisms (summarised in table 1). These techniques have complementary advantages with respect to each other. This review focuses on the technological advances used for visualization of host-pathogen interactions either in vivo, or ex vivo in whole organisms in five imaging techniques: intravital microscopy (IVM), optical projection tomography (OPT), bioluminescence imaging, optoacoustic imaging (OAI), and magnetic resonance imaging (MRI). Advanced fluorescence methods applied to intravital microscopyIntravital microscopy (IVM) is a powerful technique to investigate dynamic cellular processes and host-parasite interactions within functioning organs. Organs studied by IVM in the context of parasitology include the brain [1][2][3][4], the skin [5][6][7], the placenta [8,9], the lungs [10], the liver [11][12][13], and the spleen [14,15] (summarized in table 2, green=IVM exists; yellow=organ relevant but IVM never done; grey = IVM not done). Important advances in parasitology have been achieved using wide-field epifluorescence, confocal, spinning disc, or two-photon IVM.Recent developments, which have expanded the applications of IVM include the generation of

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Section: Optical Projection Tomography (Opt) and Selective Plane Illumentioning

confidence: 99%

Toolbox for In Vivo Imaging of Host–Parasite Interactions at Multiple Scales

Niz

Spadin

Marti

et al. 2019

Trends in Parasitology

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“…OPT can be used in a number of different applications with specimens that include embryos 2 , 3 , mouse organs 4 – 6 and plants 7 . At the same time novel OPT configurations have been presented to achieve fast acquisition 8 – 10 , to reconstruct the fluorescence lifetime and Förster resonance energy transfer contrast 11 , 12 , to obtain the contrast from blood flow 13 – 16 . In parallel, advanced recontruction algorithms have constantly been developed 17 – 19 .…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional bright-field microscopy with isotropic resolution based on multi-view acquisition and image fusion reconstruction

Calisesi

Candeo

Farina

et al. 2020

Sci Rep

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Optical Projection Tomography (OPT) is a powerful three-dimensional imaging technique used for the observation of millimeter-scaled biological samples, compatible with bright-field and fluorescence contrast. OPT is affected by spatially variant artifacts caused by the fact that light diffraction is not taken into account by the straight-light propagation models used for reconstruction. These artifacts hinder high-resolution imaging with OPT. In this work we show that, by using a multiview imaging approach, a 3D reconstruction of the bright-field contrast can be obtained without the diffraction artifacts typical of OPT, drastically reducing the amount of acquired data, compared to previously reported approaches. The method, purely based on bright-field contrast of the unstained sample, provides a comprehensive picture of the sample anatomy, as demonstrated in vivo on Arabidopsis thaliana and zebrafish embryos. Furthermore, this bright-field reconstruction can be implemented on practically any multi-view light-sheet fluorescence microscope without complex hardware modifications or calibrations, complementing the fluorescence information with tissue anatomy.

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“…OPT collects a series of projection images of the sample from different angles and computationally reconstructs a 3D image of the sample using filtered back-projection or algebraic reconstruction techniques 6 . OPT has been implemented for both fixed tissue 7 – 9 and live imaging 10 – 13 , and for applications such as developmental biology 14 , diabetes studies 8 , and immunology 15 . One of the advantages of OPT is that it can be used for fluorescent (fluorescent proteins and fluorophore-labeled antibodies) and non-fluorescent (natural pigmentations and colored dyes) contrasts.…”

Section: Introductionmentioning

confidence: 99%

Attenuation artifacts in light sheet fluorescence microscopy corrected by OPTiSPIM

et al. 2018

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Light sheet fluorescence microscopy (LSFM) is rapidly becoming an essential technology for mesoscopic imaging of samples such as embryos and adult mouse organs. However, LSFM can suffer from optical artifacts for which there is no intrinsic solution. The attenuation of light due to absorbing material causes “shadow” artifacts along both the illumination and detection paths. Several approaches have been introduced to reduce this problem, including scanning illumination and multi-view imaging. However, neither of these approaches completely eliminates the problem. If the distribution of the absorbing material is complex, shadows cannot be avoided. We introduce a new approach that relies on multi-modal integration of two very different mesoscopic techniques. Unlike LSFM, optical projection tomography (OPT) can operate in transmission mode to create a voxel map of the 3D distribution of the sample’s optical attenuation. Here, we demonstrate a hybrid instrument (OPTiSPIM) that can quantify this attenuation and use the information to correct the shadow artifacts of LSFM.

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Mesoscopic in vivo 3-D tracking of sparse cell populations using angular multiplexed optical projection tomography

Cited by 6 publications

References 17 publications

Toolbox for In Vivo Imaging of Host–Parasite Interactions at Multiple Scales

Toolbox for In Vivo Imaging of Host–Parasite Interactions at Multiple Scales

Three-dimensional bright-field microscopy with isotropic resolution based on multi-view acquisition and image fusion reconstruction

Attenuation artifacts in light sheet fluorescence microscopy corrected by OPTiSPIM

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