Mesoscopic hydrogel molding to control the 3D geometry of bioartificial muscle tissues

Bian, Weining; Liau, Brian; Badie, Nima; Bursac, Nenad

doi:10.1038/nprot.2009.155

Cited by 199 publications

(193 citation statements)

References 50 publications

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“…Large single muscle bundles and smaller tribundle muscle implants were formed within polydimethylsiloxane (PDMS) molds as previously described (26,41). Cell/hydrogel mixture (SI Appendix, Table S1) was injected into the PDMS molds, polymerized at 37°C for 45 min, and cultured on a rocker at 37°C for up to 4 wk.…”

Section: Methodsmentioning

confidence: 99%

Biomimetic engineered muscle with capacity for vascular integration and functional maturation in vivo

Juhas

Engelmayr

Fontanella

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Tissue-engineered skeletal muscle can serve as a physiological model of natural muscle and a potential therapeutic vehicle for rapid repair of severe muscle loss and injury. Here, we describe a platform for engineering and testing highly functional biomimetic muscle tissues with a resident satellite cell niche and capacity for robust myogenesis and self-regeneration in vitro. Using a mouse dorsal window implantation model and transduction with fluorescent intracellular calcium indicator, GCaMP3, we nondestructively monitored, in real time, vascular integration and the functional state of engineered muscle in vivo. During a 2-wk period, implanted engineered muscle exhibited a steady ingrowth of blood-perfused microvasculature along with an increase in amplitude of calcium transients and force of contraction. We also demonstrated superior structural organization, vascularization, and contractile function of fully differentiated vs. undifferentiated engineered muscle implants. The described in vitro and in vivo models of biomimetic engineered muscle represent enabling technology for novel studies of skeletal muscle function and regeneration.tissue engineering | contractile force | self-repair | angiogenesis | window chamber

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Section: Methodsmentioning

confidence: 99%

Biomimetic engineered muscle with capacity for vascular integration and functional maturation in vivo

Juhas

Engelmayr

Fontanella

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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209

316

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“…For example, Bian et al developed a soft lithography protocol to seed a mixture of muscle cells and fibrin gel within a poly(dimethyl siloxane) (PDMS) mold, creating www.advancedsciencenews.com www.advhealthmat.de a muscle tissue sheet with elliptical pores that improved the directionality within the muscle fibers (Figure 6). [130] Similarly, Gauvin et al used a 3D projection stereolithography technique to form a macroporous gelatin methacrylate hydrogel structures for cell growth and tissue engineering applications. [131] Weidner and co-workers have also shown that templated microchanneled/capillary hydrogels fabricated through 3D projection stereolithography are effective as anisotropic scaffolds for promoting axonal regrowth.…”

Section: Wwwadvancedsciencenewscom Wwwadvhealthmatdementioning

confidence: 99%

Structured Macroporous Hydrogels: Progress, Challenges, and Opportunities

Hoare

2017

Adv Healthcare Materials

170

168

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Structured macroporous hydrogels that have controllable porosities on both the nanoscale and the microscale offer both the swelling and interfacial properties of bulk hydrogels as well as the transport properties of “hard” macroporous materials. While a variety of techniques such as solvent casting, freeze drying, gas foaming, and phase separation have been developed to fabricate structured macroporous hydrogels, the typically weak mechanics and isotropic pore structures achieved as well as the required use of solvent/additives in the preparation process all limit the potential applications of these materials, particularly in biomedical contexts. This review highlights recent developments in the field of structured macroporous hydrogels aiming to increase network strength, create anisotropy and directionality within the networks, and utilize solvent‐free or additive‐free fabrication methods. Such functional materials are well suited for not only biomedical applications like tissue engineering and drug delivery but also selective filtration, environmental sorption, and the physical templating of secondary networks.

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“…20,21 The use of computer-assisted design to systematically alter tissue mold dimensions and introduce tissue pores with defined geometry, distribution, and direction in this system allowed us to precisely control engineered tissue thickness, shape, and local and global alignment of differentiated muscle fibers. Specifically, by fabricating elastomeric tissue molds with parallel hexagonal posts arranged in staggered fashion, we were able to create fibrin gel-based muscle networks containing: (1) elliptical pores with length that was directly determined by the post length (PL) and width that was determined by both the post width and degree of cell-mediated gel compaction, and (2) dense, cross-striated myofibers that were in average aligned along the long axis of the elliptical pores.…”

Section: Introductionmentioning

confidence: 99%

Local Tissue Geometry Determines Contractile Force Generation of Engineered Muscle Networks

Bian

Juhas

Pfeiler

et al. 2012

Tissue Engineering Part A

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The field of skeletal muscle tissue engineering is currently hampered by the lack of methods to form large muscle constructs composed of dense, aligned, and mature myofibers and limited understanding of structure-function relationships in developing muscle tissues. In our previous studies, engineered muscle sheets with elliptical pores (''muscle networks'') were fabricated by casting cells and fibrin gel inside elastomeric tissue molds with staggered hexagonal posts. In these networks, alignment of cells around the elliptical pores followed the local distribution of tissue strains that were generated by cell-mediated compaction of fibrin gel against the hexagonal posts. The goal of this study was to assess how systematic variations in pore elongation affect the morphology and contractile function of muscle networks. We found that in muscle networks with more elongated pores the force production of individual myofibers was not altered, but the myofiber alignment and efficiency of myofiber formation were significantly increased yielding an increase in the total contractile force despite a decrease in the total tissue volume. Beyond a certain pore length, increase in generated contractile force was mainly contributed by more efficient myofiber formation rather than enhanced myofiber alignment. Collectively, these studies show that changes in local tissue geometry can exert both direct structural and indirect myogenic effects on the functional output of engineered muscle. Different hydrogel formulations and pore geometries will be explored in the future to further augment contractile function of engineered muscle networks and promote their use for basic structure-function studies in vitro and, eventually, for efficient muscle repair in vivo.

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Mesoscopic hydrogel molding to control the 3D geometry of bioartificial muscle tissues

Cited by 199 publications

References 50 publications

Biomimetic engineered muscle with capacity for vascular integration and functional maturation in vivo

Biomimetic engineered muscle with capacity for vascular integration and functional maturation in vivo

Structured Macroporous Hydrogels: Progress, Challenges, and Opportunities

Local Tissue Geometry Determines Contractile Force Generation of Engineered Muscle Networks

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