Mesoscopic fluorescence molecular tomography of reporter genes in bioprinted thick tissue

Ozturk, Mehmet S.; Lee, Vivian K.; Zhao, Lingling; Dai, Guohao; Intes, Xavier

doi:10.1117/1.jbo.18.10.100501

Cited by 54 publications

(44 citation statements)

References 16 publications

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“…As the mean volume of pulp in adult teeth is approximately 0.02cm 3 with a typical diameter of ~2~3mm, LOT may be a suitable imaging modality to perform functional and molecular imaging of the teeth pulp. However, the optical properties of teeth are different with other human tissues that are used in tissue engineering [9] or in preclinical studies [10]. It is reported that the typical absorption coefficient of teeth is ~0.3-0.4mm -1 while the typical scattering coefficient is about 28mm -1 with anisotropy factor g=0.93 [11].…”

Section: Introductionmentioning

confidence: 99%

Dental imaging using laminar optical tomography and micro CT

et al. 2014

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Dental lesions located in the pulp are quite difficult to identify based on anatomical contrast, and, hence, to diagnose using traditional imaging methods such as dental CT. However, such lesions could lead to functional and/or molecular optical contrast. Herein, we report on the preliminary investigation of using Laminar Optical Tomography (LOT) to image the pulp and root canals in teeth. LOT is a non-contact, high resolution, molecular and functional mesoscopic optical imaging modality. To investigate the potential of LOT for dental imaging, we injected an optical dye into ex vivo teeth samples and imaged them using LOT and micro-CT simultaneously. A rigid image registration between the LOT and micro-CT reconstruction was obtained, validating the potential of LOT to image molecular optical contrast deep in the teeth with accuracy, non-invasively. We demonstrate that LOT can retrieve the 3D bio-distribution of molecular probes at depths up to 2mm with a resolution of several hundred microns in teeth.

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Section: Introductionmentioning

confidence: 99%

Dental imaging using laminar optical tomography and micro CT

et al. 2014

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“…Moreover, it has been developed to incorporate time as a new dimension to observe the developmental biology. Therefore, MFMT can speed up studies of functional or morphological dependencies on external stimuli or gene mutations and can further obtain the complete picture of development or tissue function [6,7].…”

Section: Introductionmentioning

confidence: 99%

Image reconstruction of mesoscopic fluorescence molecular tomography based on dual-wavelength measurements

Zou

2014

2014 12th International Conference on Signal Processing (ICSP)

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Mesoscopic fluorescence molecular tomography is a new emerging optical imaging technique. It can be used for noninvasive imaging at dimensions of 1mm-5mm. The iterative image reconstruction algorithms for mesoscopic fluorescence molecular tomography are usually computationally expensive for the existence of large number of unknowns. In this paper, a new image reconstruction algorithm for mesoscopic fluorescence molecular tomography based on dual-wavelength measurements is proposed. The proposed algorithm employs both of the excitation and emission light measurements during the iteration process. The Jacobian matrix is calculated with the Green's function. The prior information is employed to guide the generation of the adaptively refined grid. To further improve the efficiency of reconstruction, the grid is refined throughout the whole reconstruction process according to the last iterative results. Simulation results demonstrate that the proposed algorithm can greatly speed up the reconstruction process with the improved quality of the reconstructed images.

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“…25 Since then, several improvements or alternative designs have been investigated. 3,28,30,31,73,74 We first summarize two representative LOT systems in the following sections, and then LOT/MFMT with variant configurations and time-resolved protocol are also covered.…”

Section: Laminar Optical Tomography Instrumentationmentioning

confidence: 99%

“…[25][26][27][28] Soon after, it was rapidly adapted to fluorescent molecular imaging, applications termed either fluorescence laminar optical tomography (FLOT) 3,28,29 or mesoscopic fluorescence molecular tomography (MFMT). 30,31 Similar to diffuse optical tomography (DOT), 32 LOT uses an array of photon detectors or a chargecoupled device (CCD) camera to collect photons emitted from locations at different distances away from the illumination position, enabling simultaneous detection of scattered light travelling through different depths in the tissue. LOT is sensitive to both absorption and fluorescence contrasts providing an axial resolution of ∼100 to 200 μm with several millimeters imaging depth.…”

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confidence: 99%

Review of mesoscopic optical tomography for depth-resolved imaging of hemodynamic changes and neural activities

et al. 2016

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Abstract. Understanding the functional wiring of neural circuits and their patterns of activation following sensory stimulations is a fundamental task in the field of neuroscience. Furthermore, charting the activity patterns is undoubtedly important to elucidate how neural networks operate in the living brain. However, optical imaging must overcome the effects of light scattering in the tissue, which limit the light penetration depth and affect both the imaging quantitation and sensitivity. Laminar optical tomography (LOT) is a three-dimensional (3-D) in-vivo optical imaging technique that can be used for functional imaging. LOT can achieve both a resolution of 100 to 200 μm and a penetration depth of 2 to 3 mm based either on absorption or fluorescence contrast, as well as large field-of-view and high acquisition speed. These advantages make LOT suitable for 3-D depth-resolved functional imaging of the neural functions in the brain and spinal cords. We review the basic principles and instrumentations of representative LOT systems, followed by recent applications of LOT on 3-D imaging of neural activities in the rat forepaw stimulation model and mouse whisker-barrel system.

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Mesoscopic fluorescence molecular tomography of reporter genes in bioprinted thick tissue

Cited by 54 publications

References 16 publications

Dental imaging using laminar optical tomography and micro CT

Dental imaging using laminar optical tomography and micro CT

Image reconstruction of mesoscopic fluorescence molecular tomography based on dual-wavelength measurements

Review of mesoscopic optical tomography for depth-resolved imaging of hemodynamic changes and neural activities

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