Mesorhizobium comanense sp. nov., isolated from groundwater

Abstract

Strain 3P27G6T was isolated from an artesian well connected to the thermal water basin of Comano Terme, Province of Trento, Italy. In phylogenetic analyses based on multilocus sequence analysis, strain 3P27G6T clustered together with Mesorhizobium australicum WSM2073T. Genome sequencing produced a 99.51 % complete genome, with a length of 7 363 057 bp and G+C content of 63.53 mol%, containing 6897 coding sequences, 55 tRNA and three rRNA. Average nucleotide identity analysis revealed that all distances calculated between strain 3P27G6T and the other Mesorhizobium genomes were below 0.9, indicating that strain 3P27G6T represents a new species. Therefore, we propose the name Mesorhizobium comanense sp. nov. with the type strain 3P27G6T (=DSM 110654T=CECT 30067T). Strain 3P27G6T is a Gram-negative, rod-shaped, aerobic bacterium. Growth condition, antibiotic susceptibility, metabolic and fatty acid-methyl esters profiles of the strain were determined. Only few nodulation and nitrogen fixation genes were found in the genome, suggesting that this strain may not be specialized in nodulation or in nitrogen fixation.

Comparative Genome Analysis of Polar Mesorhizobium sp. PAMC28654 to Gain Insight into Tolerance to Salinity and Trace Element Stress

“…They have been isolated worldwide from terrestrial environments, especially from soil and root nodules. They have also been isolated from seawater, wastewater treatment systems, and groundwater [ 4 , 5 , 6 ]. The broad distribution of this genus and its ability to form a symbiotic relationship with several plant genera make it an interesting candidate from agronomic and ecological perspectives [ 7 , 8 ].…”

Mesorhizobium liriopis sp. nov., isolated from the fermented fruit of Liriope platyphylla a medicinal plant

“…At the time of the present study, the genus Mesorhizobium comprised 59 species with validly published names (https://lpsn.dsmz.de/genus/mesorhizobium) [3]. Mesorhizobium species have been found in diverse environments, such as soil, Sophora root nodules, Biserrula pelecinus L., groundwater, wild legumes, Astragalus adsurgens, deep seawater, Alhagi sparsifolia, Lotus corniculatus nodules and root nodules of Robinia pseudoacacia [4][5][6][7][8][9][10][11][12]. Most species of the genus Mesorhizobium can cause nodules in legumes where nitrogen fixation is performed [13].…”