Mesorhizobium atlanticum sp. nov., a new nitrogen-fixing species from soils of the Brazilian Atlantic Forest biome

Helene, Luisa Caroline Ferraz; Dall’Agnol, Rebeca Fuzinatto; Delamuta, Jakeline Renata Marçon; Hungría, Mariangela

doi:10.1099/ijsem.0.003397

Cited by 20 publications

(5 citation statements)

References 24 publications

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“…The only respiratory quinone identified in strain MaA-C15 T was Q-10, in agreement with those of other species of the genus Mesorhizobium [2–6]. C 16 : 0 , 11-methyl-C 18 : 1 ω 7 c , cyclo-C 19 : 0 ω 8 c and summed feature 8 (C 18 : 1 ω 6 c and/or C 18 : 1 ω 7 c ) were identified in strain MaA-C15 T as the major cellular fatty acids (>5 % of the total fatty acids) ().…”

Section: Physiology and Chemotaxonomysupporting

confidence: 78%

“…Phosphatidylethanolamine, phosphatidylmonomethylethanolamine, an unidentified aminophospholipid, an unidentified glycolipid and three unidentified phospholipids were detected as the major polar lipids (Fig. S4), which was different from other closely related members of the genus Mesorhizobium [2–11]. For instance, one of the reference strains, M.…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

“…The genus Mesorhizobium , known to have the ability to engage in nitrogen-fixing endosymbiosis with leguminous plants, was first proposed as a member of the family Phyllobacteriaceae through reclassifying five Rhizobium species based on fatty acid composition and phylogenetic analysis of 16S rRNA gene sequences in 1997 [1]. At the time of writing, the genus Mesorhizobium comprises 57 validly published species that have been mostly isolated from root nodules of various host plants in soil environments [2–11]. Only two Mesorhizobium species were isolated from marine habitats [12, 13] and currently no Mesorhizobium type strain has been isolated from freshwater.…”

Section: Introductionmentioning

confidence: 99%

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Mesorhizobium microcysteis sp. nov., isolated from a culture of Microcystis aeruginosa

Jung

Seo

Kim

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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Strain MaA-C15T, a Gram-stain-negative, non-spore-forming and strictly aerobic bacterium, was isolated from a xenic culture of Microcystis aeruginosa in the Republic of Korea. Cells were motile rods showing positive reactions in catalase and oxidase tests. Growth was observed between 15 and 37 °C (optimum, 30 °C), between pH 6.0 and pH 11.0 (optimum, pH 7.5) and in the presence of 0–2.0 % (w/v) NaCl (optimum, 0 %). Strain MaA-C15T contained C16 : 0, 11-methyl-C18 : 1 ω7c, cyclo-C19 : 0 ω8c and summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) as the major cellular fatty acids and ubiquinone-10 as the sole respiratory quinone. Phosphatidylethanolamine, phosphatidylmonomethylethanolamine, an unidentified aminophospholipid, an unidentified glycolipid and three unidentified phospholipids were detected as the major polar lipids. The G+C content of the genomic DNA was 64.1 mol%. Phylogenetic and phylogenomic analyses based on 16S rRNA gene and genome sequences revealed that strain MaA-C15T formed a phyletic lineage with Mesorhizobium sediminum YIM M12096T within the family Phyllobacteriaceae . Strain MaA-C15T was most closely related to Mesorhizobium albiziae DSM 21822T with a 98.2 % 16S rRNA sequence similarity. Average nucleotide identity and in silico DNA–DNA hybridization values between strain MaA-C15T and M. albiziae DSM 21822T were 75.4 and 20.1 %, respectively. Based on the results of phenotypic, chemotaxonomic and molecular analyses, strain MaA-C15T represents a novel species of the genus Mesorhizobium , for which the name Mesorhizobium microcysteis sp. nov. is proposed. The type strain is MaA-C15T (=KACC 21226T=JCM 33503T).

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Section: Physiology and Chemotaxonomysupporting

confidence: 78%

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mesorhizobium microcysteis sp. nov., isolated from a culture of Microcystis aeruginosa

Jung

Seo

Kim

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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“…As a result, each described taxon is represented by a lower taxon, and a type species represents each genus. Nowadays, the advancement of next-generation sequencing (NGS) has encouraged the description of novel species based mainly on genomic sequences [ 18 ], and this practice is revolutionizing the bacterial species concept and the taxonomic groups [ 19 – 23 ].…”

Section: The Bacteria Species Concept and Description Of New Taxamentioning

confidence: 99%

New Insights into the Taxonomy of Bacteria in the Genomic Era and a Case Study with Rhizobia

Helene

Klepa

Hungría

2022

International Journal of Microbiology

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Since early studies, the history of prokaryotes taxonomy has dealt with many changes driven by the development of new and more robust technologies. As a result, the number of new taxa descriptions is exponentially increasing, while an increasing number of others has been subject of reclassification, demanding from the taxonomists more effort to maintain an organized hierarchical system. However, expectations are that the taxonomy of prokaryotes will acquire a more stable status with the genomic era. Other analyses may continue to be necessary to determine microbial features, but the use of genomic data might be sufficient to provide reliable taxa delineation, helping taxonomy to reach the goal of correct classification and identification. Here we describe the evolution of prokaryotes’ taxonomy until the genomic era, emphasizing bacteria and taking as an example the history of rhizobia taxonomy. This example was chosen because of the importance of the symbiotic nitrogen fixation of legumes with rhizobia to the nitrogen input to both natural ecosystems and agricultural crops. This case study reports the technological advances and the methodologies used to classify and identify bacterial species and indicates the actual rules required for an accurate description of new taxa.

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“…For nodC genes, primers were used: nodCF (5ʹ-AYGTHGTYGAYGACGGTTC -3ʹ) / nodCI (5ʹ-CGYGACAGCCANTCKCTATTG -3ʹ) (Laguerre et al, 2001) and the PCR protocol as described earlier (Wei, Young and Bontemps, 2009). For nodD genes, primers were used: (5ʹ-GCGAACGYWTTCTGACACC-3ʹ) / (5ʹ-TCSGTAAATSCSGGAAG -3ʹ) and the PCR protocol as described earlier (Ji et al, 2015). The strains Mesorhizobium japonicum Opo-235 and Opo-242, as well as the strain M. kowhaii Ach-343 (Safronova et al, 2018; were used as reference strains.…”

Section: Amplification Of Nodc and Nodd Genes In Rhizobial Isolatesmentioning

confidence: 99%

Rhizobial isolates in active layer samples of permafrost soil of Spitsbergen, Arctic

Karlov

Sazanova

Kuznetsova

et al. 2021

BioComm

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Twenty-nine strains were isolated from two samples of the permafrost active layer of the Spitsbergen archipelago. The estimated number of bacteria ranged from 4.0⋅104 to 1.7⋅107 CFU∙g-1. As a result of sequencing of the 16S rRNA (rrs) genes, the isolates were assigned to 13 genera belonging to the phyla Actinobacteria, Proteobacteria (classes α, β, and γ), Bacteroidetes, and Firmicutes. Six isolates belonged to the rhizobial genus Mesorhizobium (order Rhizobiales). A plant nodulation assay with seedlings of legume plants Astragalus norvegicus, A. frigidus, A. subpolaris and Oxytropis sordida, originated from Khibiny (Murmansk region, Russia) and inoculated with Mesorhizobium isolates, showed the inability of these strains to form nodules on plant roots. Symbiotic (sym) genes nodC and nodD were not detected in Mesorhizobium strains either.

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Mesorhizobium atlanticum sp. nov., a new nitrogen-fixing species from soils of the Brazilian Atlantic Forest biome

Cited by 20 publications

References 24 publications

Mesorhizobium microcysteis sp. nov., isolated from a culture of Microcystis aeruginosa

Mesorhizobium microcysteis sp. nov., isolated from a culture of Microcystis aeruginosa

New Insights into the Taxonomy of Bacteria in the Genomic Era and a Case Study with Rhizobia

Rhizobial isolates in active layer samples of permafrost soil of Spitsbergen, Arctic

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