Mesoporous Silica Particles Functionalized with Newly Extracted Fish Oil (Omeg@Silica) Reducing IL-8 Counteract Cell Migration in NSCLC Cell Lines

Abstract

Lung cancer is one of the leading forms of cancer in developed countries. Interleukin-8 (IL-8), a pro-inflammatory cytokine, exerts relevant effects in cancer growth and progression, including angiogenesis and metastasis in lung cancer. Mesoporous silica particles, functionalized with newly extracted fish oil (Omeg@Silica), are more effective than the fish oil alone in anti-proliferative and pro-apoptotic effects in non-small cell lung cancer (NSCLC) cell lines. The mechanisms that explain this efficacy are not yet understood. The aim of the present study is therefore to decipher the anti-cancer effects of a formulation of Omeg@Silica in aqueous ethanol (FOS) in adenocarcinoma (A549) and muco-epidermoid (NCI-H292) lung cancer cells, evaluating cell migration, as well as IL-8, NF-κB, and miRNA-21 expression. Results show that in both cell lines, FOS was more efficient than oil alone, in decreasing cell migration and IL-8 gene expression. FOS reduced IL-8 protein release in both cell lines, but this effect was only stronger than the oil alone in A549. In A549, FOS was able to reduce miRNA-21 and transcription factor NF-κB nuclear expression. Taken together, these data support the potential use of the Omeg@Silica as an add-on therapy for NSCLC. Dedicated studies which prove clinical efficacy are needed.

How Key Alterations of Mesoporous Silica Nanoparticles Affect Anti-Lung Cancer Therapy? A Comprehensive Review of the Literature

“… [ 75 ] 6 Extracted fish oil - - To analyze cell migration, IL-8, NF-B, and miRNA-21 expression in adenocarcinoma (A549) and mucoepidermoid (NCI-H292) lung cancer cells in order to determine the anti-cancer effects of a formulation of omega-silica in aqueous ethanol. [ 76 ] 7 Bortezomib - Solvent evaporation To create and develop hollow, mesoporous silica nanospheres encapsulated with bortezomib as a potent drug delivery method. [ 77 ] 8 Curcumin - Wet impregnation To create curcumin/mesoporous silica SBA-15 composite particles for pulmonary administration of treatment for metastatic lung cancer.…”

“…There is a need for focused investigations that demonstrate clinical efficacy. 76 The use of hollow mesoporous silicon nanospheres (HMSNs) to encapsulate BTZ for drug delivery is described in this work. The half-maximum inhibitory concentration (IC50) of HMSNseBTZ was 42% of that for free BTZ in 48 h treatments in an in vitro cell viability experiment on human NSCLC H1299 cells.…”

Amino Acids in New Organic Fertilizer AnchoisFert

“…[1] Besides being rich in omega-3 lipids and vitamin D in bioavailable vitamin D 3 isomer form, [3] that make it suitable to replace highly refined fish oil used to produce omega-3 dietary supplements, [4] the "AnchoisOil" whole fish oil co-extracted with AnchoisFert microencapsulated in mesoporous silica has even shown powerful anticancer activity in vitro, [5] which has lately been ascribed to its ability to decrease IL-8 gene expression, miRNA-21 and transcription factor NF-kB nuclear expression. [6] Taken individually, either the production of the new whole fish oil [7] and that of the new organic fertilizer [8] are technically feasible and economically convenient. In brief, thanks to the "LimoFish" circular economy process, biowaste available in huge amounts (anchovy is the world's most caught fish) is converted into valued bioproducts in high demand using a non-toxic solvent derived from orange peel (limonene) which is nearly entirely recovered after the oil and fertilizer coproduction.…”

“…Besides being rich in omega‐3 lipids and vitamin D in bioavailable vitamin D 3 isomer form, [3] that make it suitable to replace highly refined fish oil used to produce omega‐3 dietary supplements, [4] the “AnchoisOil” whole fish oil co‐extracted with AnchoisFert microencapsulated in mesoporous silica has even shown powerful anticancer activity in vitro , [5] which has lately been ascribed to its ability to decrease IL‐8 gene expression, miRNA‐21 and transcription factor NF‐κB nuclear expression [6] …”