Mesoporous silica-encapsulated gold nanoparticles as artificial enzymes for self-activated cascade catalysis

Lin, Youhui; Li, Zhenhua; Chen, Zhaowei; Ren, Jinsong; Qu, Xiaogang

doi:10.1016/j.biomaterials.2013.01.007

Cited by 215 publications

(186 citation statements)

References 45 publications

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“…In our experiments, a high density of very small and stable AuNPs encapsulated in an expanded mesoporous silica support (Au-SiO 2 nanoparticles) was synthesized using our recently developed method (see details in the Experimental procedures and Supplementary Figures S1 and S2). 33 Next, the peroxidase-mimicking activity of the Au-SiO 2 nanocatalyst in solution was evaluated. Similar to the enzyme horseradish peroxidase (HRP), the Au-SiO 2 nanocatalyst was able to catalyze the reaction of the peroxidase substrate ABTS in the presence of H 2 O 2 to produce a colored product, ABTS Á þ , that was accompanied by an increase in the absorption peak at 417 nm (Figures 1a and b).…”

Section: Resultsmentioning

confidence: 99%

“…33 Briefly, the above-mentioned nanoparticles (100 mg) were dispersed in 10 ml of distilled water by sonication for 10 min (100 mg), followed by the addition of the HAuCl 4 solution (1 ml; 20 mM). After 10 min of sonication, freshly prepared NaBH 4 (5 ml; 0.1 M) was added into the aqueous solution with vigorous stirring.…”

Section: Preparation Of Au-sio 2 Nanoparticlesmentioning

confidence: 99%

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Incorporating ATP into biomimetic catalysts for realizing exceptional enzymatic performance over a broad temperature range

Lin

Ren

2014

NPG Asia Mater

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There has been great interest in the development of artificial inorganic nanomaterials that mimic natural peroxidases. Unfortunately, these nanomaterials usually possess relatively low catalytic activity and are generally considered to work effectively only within a narrow temperature range (for example, they are inactive at high temperatures). Intriguingly, adenosine triphosphate (ATP) is not only the ubiquitous energy currency of life, it is also known to form charge transfer complexes with aromatic molecules and to participate in free radical redox chemistry. Inspired by its unique properties, for the first time, we reveal a novel catalytic role for ATP and show that ATP is an ideal boosting agent for markedly improving the catalytic activity of a peroxidase mimic over a broad temperature range and, more significantly, making it possible to achieve exceptionally efficient high-temperature catalytic reactions. These observations pave the way for identifying highly effective modulators that promote the overall performance of artificial enzymes. NPG Asia Materials (2014) 6, e114; doi:10.1038/am.2014.42; published online 18 July 2014 INTRODUCTION Natural enzymes, which are biological molecules of immense catalytic force and high substrate specificity, have attracted considerable attention for use in pharmaceutical processes, agrochemical production, biosensing and food industry applications. 1-3 However, their applications are largely limited by their intrinsic properties, such as the sensitivity of catalytic activity to environmental conditions and low operational stability (owing to denaturation and digestion), as well as by the high costs of preparation and purification. 4,5 To circumvent the aforementioned limitations, tremendous efforts have been made to develop biomimetic catalysts. Among the countless examples of artificial enzymes, the emergence of and recent advances in nanotechnology and biology provide new opportunities for designing functional nanomaterials with enzyme-like characteristics. 4,[6][7][8] These materials might serve as novel and promising artificial enzymes with the advantages of facile preparation, tunability of catalytic activity and high stability under stringent conditions. Owing to the excellent catalytic properties of these nanomaterials, Scrimin and co-workers 7 called them 'nanozymes' in analogy to the nomenclature of catalytically active polymers (synzymes). Magnetic nanoparticles, 4,9 CeO 2 , 6,10-12 V 2 O 5 , 13,14 gold nanoparticles, 7,8,[15][16][17][18] PtPd ÀFe 3 O 4 , 19,20 carbon nanotubes, 21,22 graphene oxide and graphene nanocomposites 23,24 and other types of nanoparticle have been found to exhibit unique enzyme-like catalytic activities and have

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Section: Resultsmentioning

confidence: 99%

Section: Preparation Of Au-sio 2 Nanoparticlesmentioning

confidence: 99%

Incorporating ATP into biomimetic catalysts for realizing exceptional enzymatic performance over a broad temperature range

Lin

Ren

2014

NPG Asia Mater

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“…But the metal nanoparticles-based mimetic enzymes are extremely sensitive to surface properties (Lin et al, 2013). When metal nanoparticles are covered with the polymer films, only small fractions of catalytic sites are exposed to the surface, seriously affecting the catalytic efficiency.…”

Section: Introductionmentioning

confidence: 99%

Biomimetic sensor based on molecularly imprinted polymer with nitroreductase-like activity for metronidazole detection

Yan

Liu

et al. 2016

Biosensors and Bioelectronics

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“…Up to now, various metal and metal oxide nanomaterials have been found to exhibit intrinsic peroxidase-like activities, such as Au, 7,8 Pt, 9,10 Pd, 11 CuO, 12,13 The material used to modify electrode is another key point for ultrasensitive detection in electrochemical immunosensors. Multi-walled carbon nanotubes (MWCNTs) and graphene oxide (GO) are widely used as transducers for sensing enhancement because of their unique nanostructures and excellent physical properties.…”

Section: Introductionmentioning

confidence: 99%

Nonenzymatic Electrochemical Immunosensor Using Ferroferric Oxide–Manganese Dioxide–Reduced Graphene Oxide Nanocomposite as Label for α-Fetoprotein Detection

Jian

Wang

Chen

et al. 2016

NANO

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A novel nonenzymatic electrochemical immunosensor was fabricated for quantitative detection of -fetoprotein (AFP). The immunosensor was constructed by modifying gold electrode with electrochemical reduction of graphene oxide-carboxyl multi-walled carbon nanotube composites (ERGO-CMWCNTs) and electrodeposition of gold nanoparticles (AuNPs) for e®ective immobilization of primary antibody (Ab 1 Þ. Ferroferric oxide-manganese dioxide-reduced graphene oxide nanocomposites (Fe 3 O 4 @MnO 2 -rGO) were designed as labels for signal ampli¯cation. On one hand, the excellent electroconductivity and outstanding electron transfer capability of ERGO-CMWCNTs/AuNPs improved the sensitivity of the immunosensor. On the other hand, introduction of rGO could not only increase the speci¯c surface area for immobilization of secondary antibody (Ab 2 Þ but also build a synergetic e®ect to reinforce the electrocatalytic properties of catalysts. Fe 3 O 4 @MnO 2 -rGO nanocomposites were characterized by scanning electron microscope, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. Using AFP as a model analyte, the proposed sandwich-type electrochemical immunosensor exhibited a wide linear range of 0.01-50 ng Á mL À1 with a low detection limit of 5.8 pg Á mL À1 . Moreover, the Fe 3 O 4 @MnO 2 -rGO-based peroxidase mimetic system displayed an excellent analytical performance with low cost, satisfactory reproducibility and high selectivity, which could be further extended for detecting other disease-related biomarkers.

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Mesoporous silica-encapsulated gold nanoparticles as artificial enzymes for self-activated cascade catalysis

Cited by 215 publications

References 45 publications

Incorporating ATP into biomimetic catalysts for realizing exceptional enzymatic performance over a broad temperature range

Incorporating ATP into biomimetic catalysts for realizing exceptional enzymatic performance over a broad temperature range

Biomimetic sensor based on molecularly imprinted polymer with nitroreductase-like activity for metronidazole detection

Nonenzymatic Electrochemical Immunosensor Using Ferroferric Oxide–Manganese Dioxide–Reduced Graphene Oxide Nanocomposite as Label for α-Fetoprotein Detection

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