Mesoporous hybrid membrane based label free electrochemical immunoassay

Abstract

We demonstrated an application of a meso porous-silica hybrid membrane (MHM) toward label free electrochemical immunoassay. Mesoporous silica oriented parallel in the colunmar pores of a commercial anodic alumina membrane was firstly prepared. The hybrid membrane was further functionalized with antibiodies and followed by interacting with pathogenic samples through immunorecognition event. The sensing mechanism relies on the pore blocking from bound bacteria. The blockage could be traced by measuring the decrease in differential pulse voltammetric (DPV) current of ferrocenecarboxylic redox indicator. Herein, we are interested in proof of the concept on characterization of the constructed membrane, testing immunoreactivity on the membrane by ELISA and the detection of the target. The results showed the potential advantage of using this composite nanomaterial for enhancing the sensing response in the application of label free immunoassay.