Mesophyll conductance decreases in the wild type but not in an <scp>ABA</scp>‐deficient mutant (<scp>aba1</scp>) of <scp>N</scp>icotiana plumbaginifolia under drought conditions

Mizokami, Yusuke; Noguchi, Ko; Kojima, Mikiko; Sakakibara, Hitoshi; Terashima, Ichiro

doi:10.1111/pce.12394

Cited by 57 publications

(63 citation statements)

References 49 publications

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“…Mizokami et al (2015) attempted to correct calculated c i in Nicotiana plumbaginifolia using cuticle properties from grape (Vitis vinifera). Because grape had a low cuticle conductance for water vapor, they concluded the correction made little difference.…”

Section: Introductionmentioning

confidence: 99%

Impact of cuticle on calculations of the CO2 concentration inside leaves

Boyer

2015

Planta

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Water vapor over-estimates the CO 2 entering leaves during photosynthesis because the cuticle and epidermis transmit more water vapor than CO 2 . Direct measurements of internal CO 2 concentrations may be preferred. The CO2 concentration inside leaves (c i) is typically calculated from the relationship between water vapor diffusing out while CO2 diffuses in. Diffusion through the cuticle/epidermis is usually not considered. This study was undertaken to determine how much the calculations would be affected by including cuticle properties. Previous studies indicate that measurable amounts of CO2 and water vapor move through the cuticle, although much less CO2 than water vapor. The present experiments were conducted with sunflower (Helianthus annuus L) leaves in a gas exchange apparatus designed to directly measure c i, while simultaneously calculating c i. Results showed that, in normal air, calculated c i were always higher than directly measured ones, especially when abscisic acid was fed to the leaves to close the stomata and cause gas exchange to be dominated by the cuticle. The effect was attributed mostly to the reliance on the gas phase for the calculations without taking cuticle properties into account. Because cuticle properties are usually unknown and vary with the turgor of the leaf, which can stretch the waxes, it is difficult to include cuticle properties in the calculation. It was concluded that direct measurement of c i may be preferable to the calculations.

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Section: Introductionmentioning

confidence: 99%

Impact of cuticle on calculations of the CO2 concentration inside leaves

Boyer

2015

Planta

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“…However, a recent analysis with woody plants claimed that the decrease in g s was also due to the changes in leaf hydraulics and leaf turgor (Rodriguez‐Dominguez et al, ). We reported the decrease in g m with the increase in the leaf ABA content under drought conditions using the wild type and an ABA‐deficient mutant of Nicotiana plumbaginifolia (Mizokami, Noguchi, Kojima, Sakakibara, & Terashima, ). Two major findings were as follows: (a) g s and g m concertedly decreased to rising foliar ABA levels in a dose‐dependent manner.…”

Section: Introductionmentioning

confidence: 99%

Effects of instantaneous and growth CO₂ levels and abscisic acid on stomatal and mesophyll conductances

Mizokami

Noguchi

Kojima

et al. 2018

Plant Cell & Environment

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C3 photosynthesis is often limited by CO2 diffusivity or stomatal (gs) and mesophyll (gm) conductances. To characterize effects of stomatal closure induced by either high CO2 or abscisic acid (ABA) application on gm, we examined gs and gm in the wild type (Col‐0) and ost1 and slac1‐2 mutants of Arabidopsis thaliana grown at 390 or 780 μmol mol−1 CO2. Stomata of these mutants were reported to be insensitive to both high CO2 and ABA. When the ambient CO2 increased instantaneously, gm decreased in all these plants, whereas gs in ost1 and slac1‐2 was unchanged. Therefore, the decrease in gm in response to high CO2 occurred irrespective of the responses of gs. gm was mainly determined by the instantaneous CO2 concentration during the measurement and not markedly by the CO2 concentration during the growth. Exogenous application of ABA to Col‐0 caused the decrease in the intercellular CO2 concentration (Ci). With the decrease in Ci, gm did not increase but decreased, indicating that the response of gm to CO2 and that to ABA are differently regulated and that ABA content in the leaves plays an important role in the regulation of gm.

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“…With infinite g m , a single value of V Cmax adequately fits all P N vs. C i points in the lower C i range (Bunce 2010), greatly simplifying modeling. There is currently debate about whether apparent variation in g m with C i is real or a measurement artefact (Flexas et al 2007, Tholen et al 2012, Mizokami et al 2015. Bernacchi et al (2003) made the point that for P N rates measured at low PPFD, using an infinite g m in photosynthesis models is unlikely to cause much error, because C c and C i do not differ greatly when assimilation rates are low.…”

Section: Discussionmentioning

confidence: 98%

Light dependence of carboxylation capacity for C3 photosynthesis models

Bunce¹

2016

Photosynt.

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C 3 photosynthesis at high light is often modeled by assuming limitation by the maximum capacity of Rubisco carboxylation (V Cmax ) at low CO 2 concentrations, by electron transport capacity (J max ) at higher CO 2 concentrations, and sometimes by triose-phosphate utilization rate at the highest CO 2 concentrations. Net photosynthetic rate (P N ) at lower light is often modeled simply by assuming that it becomes limited by electron transport (J). However, it is known that Rubisco can become deactivated at less than saturating light, and it is possible that P N at low light could be limited by the rate of Rubisco carboxylation (V C ) rather than J. This could have important consequences for responses of P N to CO 2 and temperature at low light. In this work, P N responses to CO 2 concentration of common bean, quinoa, and soybean leaves measured over a wide range of temperatures and PPFDs were compared with rates modeled assuming either V C or J limitation at limiting light. In all cases, observed rates of P N were better predicted by assuming limitation by V C rather than J at limiting light both below and above the current ambient CO 2 . One manifestation of this plant response was that the relative stimulation of P N with increasing the ambient CO 2 concentration from 380 to 570 mol mol -1 did not decrease at less than saturating PPFDs. The ratio of V C to V Cmax at each lower PPFD varied linearly with the ratio of P N at low PPFD to P N at high PPFD measured at 380 mol mol -1 CO 2 in all cases. This modification of the standard C 3 biochemical model was much better at reproducing observed responses of light-limited P N to CO 2 concentrations from pre-industrial to projected future atmospheric concentrations.

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Mesophyll conductance decreases in the wild type but not in an ABA‐deficient mutant (aba1) of Nicotiana plumbaginifolia under drought conditions

Cited by 57 publications

References 49 publications

Impact of cuticle on calculations of the CO2 concentration inside leaves

Impact of cuticle on calculations of the CO2 concentration inside leaves

Effects of instantaneous and growth CO₂ levels and abscisic acid on stomatal and mesophyll conductances

Light dependence of carboxylation capacity for C<sub>3</sub> photosynthesis models

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Mesophyll conductance decreases in the wild type but not in an ABA‐deficient mutant (aba1) of Nicotiana plumbaginifolia under drought conditions

Cited by 57 publications

References 49 publications

Impact of cuticle on calculations of the CO2 concentration inside leaves

Impact of cuticle on calculations of the CO2 concentration inside leaves

Effects of instantaneous and growth CO2 levels and abscisic acid on stomatal and mesophyll conductances

Light dependence of carboxylation capacity for C<sub>3</sub> photosynthesis models

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Effects of instantaneous and growth CO₂ levels and abscisic acid on stomatal and mesophyll conductances