Mesocestoides litteratus (Batsch, 1786) (Cestoda: Cyclophyllidea: Mesocestoididae) from the red fox: Morphological and 18S rDNA characterization of European isolates

Literák, Ivan; Tenora, F.; Letková, V.; Goldová, M.; Torres, Jordi; Olson, Peter D.

doi:10.2478/s11687-006-0036-7

Cited by 22 publications

(23 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Because of its fast rate of evolution the ITS2 region continues to be applied for phylogenetic reconstructions at the species and genus levels (Alvarez and Wendel 2003;Wolf et al 2005;Ashokan and Pillai 2008). In context of the cestode group of parasites the orders Cyclophyllidea, Pseudophyllidea, Caryophyllidea and Trypanorhyncha have been assessed on the basis of morphological, ultrastructural and ontogenetic characters (Hoberg et al 1997(Hoberg et al , 2001Beveridge et al 1999;Bray et al 1999) and several controversial phylogenetic disputes of cestode parasites have been published (Haukisalmi et al 2001;Literak et al 2006). Since in contrast to the bordering regions of ribosomal subunits; sequence is not conserved evolutionarily, thus genetic differentiation is detectable even in closely related groups of organisms.…”

Section: Resultsmentioning

confidence: 99%

Molecular characterization of the Indian poultry nodular tapeworm, Raillietina echinobothrida (Cestoda: Cyclophyllidea: Davaineidae) based on rDNA internal transcribed spacer 2 region

et al. 2012

View full text Add to dashboard Cite

The nodular tapeworm, Raillietina echinobothrida is a well studied avian gastrointestinal parasite of family Davaineidae (Cestoda: Cyclophyllidea). It is reported to be the largest in size and second most prevalent species infecting chicken in north-east India. In the present study, morphometrical methods coupled with the molecular analysis of the second internal transcribed spacer (ITS2) region of ribosomal DNA were employed for precise identification of the parasite. The annotated ITS2 region was found to be 446 bp long and further utilized to elucidate the phylogenetic relationships and its species-interrelationships at the molecular level. In phylogenetic analysis similar topology was observed among the trees obtained by distance-based neighbor-joining as well as character-based maximum parsimony tree building methods. The query sequence R. echinobothrida is well aligned and placed within the Davaineidae group, with all Raillietina species well separated from the other cyclophyllidean (taeniid and hymenolepid) cestodes, while Diphyllobothrium latum (Pseudophyllidea: Diphyllobothriidae) was rooted as an out-group. Sequence similarities indeed confirmed our hypothesis that Raillietina spp. are neighboring the position with other studied species of order Cyclophyllidea against the out-group order Pseudophyllidea. The present study strengthens the potential of ITS2 as a reliable marker for phylogenetic reconstructions.

show abstract

Section: Resultsmentioning

confidence: 99%

Molecular characterization of the Indian poultry nodular tapeworm, Raillietina echinobothrida (Cestoda: Cyclophyllidea: Davaineidae) based on rDNA internal transcribed spacer 2 region

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Amplification of partial sequence of 18S rDNA was made using primers WormA (5′-GCGAATGGCTCAT TAAATCAG-3′)/1270R (5′-CCGTCAATTCCTTTA AGTTT-3′) (Littlewood and Olson 2001) in the following thermocycling conditions: 94°C/5 min-initial denaturation; 94°C/1 min, 52°C/1 min, and 72°C/2 min-40 cycles; 72°C/7 min-final extending (Literak et al 2006). The PCR reaction (25 μl) was performed usingthe following PCR mix: 2 μl of genomic DNA, 10 mM Tris-HCl, 50 mM KCl, 1.5 mM MgCl 2 , 200 μM of each dNTP, 150 pmol of each primer, and two units of Taq polymerase (Qiagen).…”

Section: Methodsmentioning

confidence: 99%

“…Studies by Literak et al (2004Literak et al ( , 2006, Tenora (2005), and Hrckova et al (2011) (Andras and Peter 2002;Segovia et al 2003;Martinez-Carrasco et al 2007;Krone et al 2008;Magi et al 2009). Additionally, almost nothing is known about the ranges of their intermediate and paratenic hosts in natural conditions (Literak et al 2004).…”

Section: Introductionmentioning

confidence: 93%

“…More than a decade of research have identified the most significant characteristics in species determination of adult tapeworms as the shape of the cirrus pouch, the position and number of testes, and the position of the female sex organs (Literak et al 2006;Priemer 1982;Tenora 2005). In larval stages isolated from intermediate hosts, the identification to the species level is impossible.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular identification of Mesocestoides spp. from intermediate hosts (rodents) in central Europe (Poland)

Zaleśny

Hildebrand

2011

Parasitol Res

View full text Add to dashboard Cite

Genus Mesocestoides is a representative of the small cyclophyllidean family Mesocestoididae that is found parasitizing the small intestine of carnivores. The life cycle of cestodes from this genus is complex and requires two intermediate hosts. Cysticercoids are produced in the first intermediate host (oribatid mites), which when eaten by the second intermediate host (mainly rodents, but also other mammalian species, birds, reptiles, or amphibians) form tetrathyridia in the body cavity. Because of the rich history of nomenclatural evaluation of Mesocestoididae, the taxonomic status within the genus Mesocestoides is still unclear. Additional problem constitute the difficulty or even the impossibility in the determination of tetrathyridia based on morphological features. Thus, the aim of our study was to identify a molecular characteristic of the isolates of Mesocestoides from the second intermediate hosts (rodents) based on nuclear and mitochondrial ribosomal DNA data. We choose to analyze metacestodes isolated from two species of rodents (Apodemus agrarius and Myodes glarolus) from different sites. As a result of amplification of 18S rDNA, we obtained partial sequences from four isolates ranging from 1,116 to 1,162 bp. In relation to mitochondrial sequence, 354 bp product of 12S rDNA was obtained from one isolate. The neighbor joining and maximum parsimony trees were constructed in order to examine the phylogenetic relationship within Mesocestoides spp. occurring in rodents from central Europe. The results of our research on the larval stages from rodents, living in a periphery of urban agglomeration as well as in an area of reserve protection, confirm the data of more frequently occurring Mesocestoides litteratus.

show abstract

“…Unfortunately, the use of these new tools has also led to submission of haplotypes without proper morphological characters for identifications (Foronda et al 2007;Padgett et al 2013). To date, only three representatives of the genus-Mesocestoides litteratus (Batsch, 1786), M. lineatus (Goeze, 1782), and M. corti (Hoeppli, 1925) (= M. vogae (Etges 1991)-can reliably be identified as valid species, using both morphological and molecular characters Gubány and Eszterbauer 1998;Nickisch-Rosenegk et al 1999;Literák et al 2006;Hrčkova et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Occurrence of Mesocestoides canislagopodis (Rudolphi, 1810) (Krabbe, 1865) in mammals and birds in Iceland and its molecular discrimination within the Mesocestoides species complex

et al. 2016

View full text Add to dashboard Cite

The life cycle of Mesocestoides tapeworms (Cestoda: Cyclophyllidea: Mesocestoididae) requires three hosts. The first intermediate host is unknown but believed to be an arthropod. The second intermediate host is a vertebrate. The primary definitive host is a carnivore mammal, or a bird of prey, that eats the tetrathyridium-infected second intermediate host. One representative of the genus, Mesocestoides canislagopodis, has been reported from Iceland. It is common in the arctic fox (Vulpes lagopus) and has also been detected in domestic dogs (Canis familiaris) and cats (Felis domestica). Recently, scolices of a non-maturing Mesocestoides sp. have also been detected in gyrfalcon (Falco rusticolus) intestines, and tetrathyridia in the body cavity of rock ptarmigan (Lagopus muta). We examined the taxonomic relationship of Mesocestoides from arctic fox, gyrfalcon, and rock ptarmigan using molecular methods, both at the generic level (D1 domain LSU ribosomal DNA) and at the specific level (cytochrome c oxidase subunit I (COI) and 12S mitochondrial DNA). All stages belonged to Mesocestoides canislagopodis. Phylogenetic analysis of the combined 12S-COI at the specific level confirmed that M. canislagopodis forms a distinct clade, well separated from three other recognized representatives of the genus, M. litteratus, M. lineatus, and M. corti/vogae. This is the first molecular description of this species. The rock ptarmigan is a new second intermediate host record, and the gyrfalcon a new primary definitive host record. However, the adult stage seemed not to be able to mature in the gyrfalcon, and successful development is probably restricted to mammalian hosts.

show abstract

Mesocestoides litteratus (Batsch, 1786) (Cestoda: Cyclophyllidea: Mesocestoididae) from the red fox: Morphological and 18S rDNA characterization of European isolates

Cited by 22 publications

References 15 publications

Molecular characterization of the Indian poultry nodular tapeworm, Raillietina echinobothrida (Cestoda: Cyclophyllidea: Davaineidae) based on rDNA internal transcribed spacer 2 region

Molecular characterization of the Indian poultry nodular tapeworm, Raillietina echinobothrida (Cestoda: Cyclophyllidea: Davaineidae) based on rDNA internal transcribed spacer 2 region

Molecular identification of Mesocestoides spp. from intermediate hosts (rodents) in central Europe (Poland)

Occurrence of Mesocestoides canislagopodis (Rudolphi, 1810) (Krabbe, 1865) in mammals and birds in Iceland and its molecular discrimination within the Mesocestoides species complex

Contact Info

Product

Resources

About